Yellow normocytic anaemia Flashcards

1
Q

What is malaria

A

Malaria is a parasitic infection caused by protozoa of the genus Plasmodium.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Epidemiology of malaria

A
  • Malaria is a serious global health problem that affects millions of people, particularly:
    • Young children under the age of 5
    • Pregnant women
    • Patients with other health conditions like HIV and AIDS
    • Travellers who have had no prior exposure to malaria
      Tropical and subtropical countries
      Most severe is plasmodium falciparum
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Aetiology of malaria

A

Protozoa of plasmodium genus:
- Plasmodium falciparum
- Plasmodium vivax
- Plasmodium malariae
- Plasmodium ovale
- Plasmodium knowlesi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Pathophysiology of malaria

A

Malaria is spread through bites from the female Anopheles mosquitoes that carry the disease.

Once the plasmodium gets into the bloodstream, it starts to infect and destroy mainly liver cells and red blood cells, which causes a variety of symptoms and sometimes even death.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Active infection of malaria

A

incubation time, which is the period of time between infection and symptom onset

Plasmodium falciparum incubates for a few days, whereas Plasmodium malariaieincubates for a few weeks.

release of tumour necrosis factor alpha and other inflammatory cytokines, causes fevers that typically occur in short bursts, and correspond to the rupture of the infected red blood cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Malaria causes haemolytic anaemia - what are the symptoms?

A
  • extreme fatigue,
  • headaches,
  • jaundice
  • splenomegaly
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What do most plasmodium infections have?

A

mild course of symptoms and are generally regarded as uncomplicated malarial infections. These typically resolve with treatment.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is recurrent malaria?

A

After recovery, some individuals can get symptoms after a period of time and divided and into 3 underlying causes recrudescence, relapse, and reinfection.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What are the 3 causes of recurrence in malaria?

A
  • Recrudescence refers to ineffective treatment that didn’t completely clear the infection
  • Relapse refers to situations where the blood was cleared of merozoites but hypnozoites persisted in the liver, and then emerged to cause more problems
  • Reinfection is when an individual was effectively treated, but a completely new infection caused a new bout of malaria
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What does plasmodium falciparum do? (complicated malaria)

A

known for causing the worst infections.

PF generates a sticky protein that coats the surface of the infected red blood cells.

protein causes the red blood cells to clump together and occlude tiny blood vessels - a process called cytoadherence.

Causes organ failure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Signs of malaria

A
  • Pallor: due to anaemia
  • Jaundice: due to unconjugated bilirubin from destruction of RBCs
  • Hepatosplenomegaly: due to compensation for anaemia
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Symptoms of malaria

A
  • Fever, sweats and rigors (occurs in spikes)
  • Fatigue: due to anaemia
  • Headaches
  • Myalgia
  • Vomiting
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Investigations for malaria

A
  • FBC, U&E, LFT: thrombocytopenia, elevated lactate dehydrogenase levels due to haemolysis, and normochromic, normocytic anaemia
  • Malaria blood film: will show the parasites, the concentration and also what type they are.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Management of uncomplicated malaria

A
  1. Artemether with lumefantrine (Riamet)
  2. Proguanil and atovaquone (Malarone)
  3. Quinine sulphate
  4. Doxycycline
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Complicated or severe malaria management

A

IV:

  1. Artesunate: this is the most effective treatment but is not licensed.
  2. Quinine dihydrochloride
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Prevention of malaria:

A
  • Full body clothing
  • Mosquito repellent
  • Sleeping in insecticide covered mosquito nets
  • Indoor insecticide sprays
  • Clean water
  • Use antimalarials e.g. malarone, mefloquine, doxycycline
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Complications for complicated malaria

A
  • Cerebral malaria: altered mental status, seizures and coma
  • Bilious malaria: diarrhoea, vomiting, jaundice and liver failure
  • Acute kidney injury
  • Pulmonary oedema
  • Disseminated intravascular coagulopathy (DIC)
  • Severe haemolytic anaemia
  • Multi-organ failure and death
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What is the definition of Hereditary spherocytosis (HS)?

A

inherited haemolytic anaemia and is autosomal dominant in the majority of cases (75%), but can also be autosomal recessive.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Epidemiology of HS

A
  • HS is the most common genetic haemolytic disease.
  • It is more common in Northern Europe and North America but can affect people of any race.
  • It is diagnosed in 1 in 2000 people, whilst a large proportion of these individuals are asymptomatic
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

RFs for HS

A
  • amily history
  • Northern European descent
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Why does HS occur?

A

Defect in red cell membrane proteins (ankyrin + spectrin) > causes RBC to lose biconcave shape and become spherical > leads to accelerated degradation of RBCs in spleen resulting in normocytic anaemia

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Why does splenomegaly occur in HS?

A

spleen has to work harder (hypersplenism) to clear out the abnormal RBCs and their products.

23
Q

What happens as haemolysis occurs

A

Patients can have episodes of haemolytic crisis, often triggered by infections, where the haemolysis, anaemia and jaundice is more significant.

24
Q

Patients with HS can have aplastic crisis - what is this?

A

During aplastic crisis there is increased anaemia, haemolysis and jaundice, without the normal response from the bone marrow of creating new red blood cells. This is often triggered by infection with parvovirus.

25
Q

S+S of HS

A

Splenomegaly
Pallor
Jaundice
RUQ pain
Failure to thrive
Fatigue

26
Q

1st line investigations for HS

A
  • FBC:normocytic anaemia with an increased reticulocyte count and raised MCHC
    • MCHC is increased as spherical RBCs lead to water diffusing out of the cell
  • Blood film:spherocytosis
  • LFTs:increased (unconjugated) bilirubin due to haemolysis
  • Coombs test: - important
    No tests needed if FH of HS + Typical clinical features
27
Q

Management of HS

A

Phototherapy transfusion
Blood transfusion
Folic acid
Splenectomy - reduces haemolysis

28
Q

Complications of HS

A

Gallstones
Aplastic crisis
Bone marrow expansion
Post- splenectomy sepsis

29
Q

What is G6PD deficiency?

A

G6PD deficiency is a condition where there is a defect in the G6PD enzyme normally found in all cells in the body.

30
Q

Epidemiology of G6PD

A
  • t is inherited in an X linked recessive pattern, meaning it usually affects males.
  • It is more common in Mediterranean, Middle Eastern and African patients.
  • 6DPD deficiency can be protective against malaria
31
Q

RFs for G6PD deficiency

A

Soy products
Fava beans
Red wine
Metabolic acidosis
Medications - sulfonyureas, trimethoprim, nitrofurantoin

32
Q

What is G6PD mutation caused by?

A

G6PD gene which is found on the X chromosome and thus it’s an X-linked recessive genetic condition and it almost exclusively manifests as a disease in men.

33
Q

What do low levels of G6PD lead to

A

auses low levels of NADPH, leading to low levels of reduced glutathione.

G6PD is the only way for red blood cells to get NADPH so they are especially susceptible to damage caused by free radicals.
When FR build up cell mem becomes unstable causing haemolysis

34
Q

Free radicals damage Hb, what does it do?

A

Damaged Hb precipitates inside cells and called Heinz bodies

spleen macrophages notice these Heinz bodies and try to remove them by taking a chunk out of the cells, leaving these red blood cells partially devoured. These are known as bite cells.

35
Q

When haemolysis occurs what happens?

A

this leads to conversion to bilirubin, which can result in jaundice and further complications e.g. gallstones. Some of the bilirubin is converted to urobilin, which builds up to give the urine a dark tea-like colour. This could cause damage to the kidneys

36
Q

S+S OF G6PD deficiency

A

Jaundice
Pallor
Splenomgealy
Dizziness
SOB
Headaches

37
Q

Investogations of G6PD

A
  • FBC: low levels of RBC, high reticulocytes
  • Blood film: heinz bodies and bite cells
  • LDH: elevated
  • Bilirubin: elevated
  • Haptoglobin: low
  • Coomb’s test: negative (used to detect immune mediated anaemias)
  • Diagnosis can be made by doing aG6PD enzyme assay
38
Q

Management of G6PD

A
  • Avoid trigger of haemolysis e.g. fava beans and certain medications
  • In certain cases, transfusions may be needed
39
Q

Definition of sickle cell anaemia

A

Sickle cell anaemia is an autosomal recessive mutation in the beta chain of haemoglobin, resulting in sickling of red blood cells (RBCs) and haemolysis.

40
Q

What is the epidemiology of Sickle cell anaemia?

A

The prevalence of sickle cell trait in sub-Saharan Africa is the highest in the world. This may be because it is protective against malaria.

41
Q

RFs of Sickle cell

A
  • African: 8% of black people carry the sickle cell gene
  • Family history: autosomal recessive pattern
  • Triggers of sickling: dehydration, acidosis, infection, and hypoxia
42
Q

What is sickle cell caused by

A

defective haemoglobin. Specifically, the β-globin chains end up misshapen. This is because of a mutation in the beta globin gene, or HBB gene.

1 gene mutation - sickle cell trait - if both mutated sickle cell dissease

43
Q

What happens due to the abnormal beta chain in SCA?

A

Sickle trait - reduced levels of HbA
Sickle disease - Absent HbA - has HbS - 2 A and 2 abnormal B chains - prone to siclking and haemolyss

44
Q

HbS and oxygen

A

Carries it well
When deoxygenated changes shape allowing it to aggregate with other HbS proteins forming long polymers distorting RBC into crescent shape - Sickling

45
Q

What is sickling triggered by

A

dehydration, acidosis, infection, and hypoxia. HbAS(trait) patients sickle at PaO22.5 - 4 kPa, whilstHbSS(disease) patients at PaO25 - 6 kPa.

46
Q

What does repeated sickling promote

A

Damages cell membrane - promotes premature destruction - haemolysis - leads to anaemia + free haemoglobin

47
Q

What happens with free haemoglobin

A

Free haemoglobin in plasma bound by haptoglobin and gets recycled

Recycling of that heme group yields unconjugated bilirubin, which at high concentration can cause scleral icterus, jaundice, and gallstones.

48
Q

BM + SCA

A

To counteract the anaemia of sickle cell disease, the bone marrow makes increased numbers of reticulocytes. This can cause the bones to enlarge

Splenomegaly also happens

49
Q

RBCs get stuck in capillaries causing vaso occlusion in sickled form - what can this lead to?

A

dactylitis, priapism, acute chest syndrome, stroke, depending on where the occlusion is

50
Q

S + S of SCA

A

Pain
Anaemia symptoms
Jaundice
Sequestrastion, aplastic, haemolytic and vaso-occlusive crisis

51
Q

Primary investigations for SCA

A

Newborn screening
FBC
Bloodfilm
Hb electrophoresis + solubility

52
Q

Acute management of Anaemia

A

Analgesia
Hydration
Oxygen
Antibiotics
Blood transfusion

53
Q

Chronic management of Sickle cell

A

Pain management
Hydroxycarbamide - increases level of HbF
Lifelong phenoxymethylpenicillin
Vaccinations
Blood transfusion
BM transpant