Yeasts and molds (Saccharomyces Cerevisiae) Flashcards
What is a fungus?
Any group of spore-producing organisms feeding on organic matter, including molds, yeast, mushrooms and toadstools,
What is a mold?
A fungus that grows in the form of multicellular filaments called hyphae
What is a yeast?
A microscopic fungus consisting of single oval cells that reproduce by budding, and are capable of converting surgar into alcohol and carbon dioxide
S.Cerevisiae is a species of ____________ yeast
budding
In nature Sc is generally found on the surfaces of __________
ripe fruits
Sc has _______ chromosomes
16
Sc is known for its ability to utilize ________ in formation of ethanol and other by-products
carbohydrates
In the Gay-Lussac Equation 180g sugar is converted to ____g Ethanol and ___g CO2
92, 88
Sc can exist in both a ________ or a ________ (think of its cell cycle)
haploid, diploid
If _____________ are present both haploids and diploids can undergo repeated rounds of vegetative growth and mitosis
adequate nutrients
When nutrients become depleted, both haploids and diploids arrest as ______________
stationary phase cells
Stationary phase cells are ____________ and __________ distinct from proliferating yeast cells
morphologically and biochemically
Stationary phase cells round and bright and contain much higher levels of ____________________ than proliferating cells
storage carbohydrates (trehalose and glycogen)
TRUE OR FALSE: Stationary phase cells also have an increased resistance to a number of stresses and environmental conditions when compared to growing cells
TRUE
Haploid cells can exist in one of two mating types ______
a or 𝝰
Mating type a produce a pheromone _______
Mating type 𝝰 produce a pheromone ______
a factor
𝝰 factor
Each cell type has the surface receptor for the __________ mating type. If the surface receptor is stimulated the cells arrest in the _________ of their cell cycle
opposite
G1 phase
When the haploid cells arrest in the G1 phase of growth they no longer proliferate but start ________________. The resultant shape is called________
growing towards each other
Shmoo
Eventually there is cell contact, _________, and a diploid is formed
fusion
Diploid cells that are starved of nitrogen will undergo _________ and ________
meiosis and spore formation
4 haploid spores are formed and contained in an _____
ascus, an ascus has even greater resistance to environmental factors than stationary phase cells
If the spores are returned to a rich environment they will _________ and _____________ as haploid cells
germinate and commence growth
Sc vegetative proliferation occurs via __________
budding
The cell cycle comprises several ______________where the progression of the cell cycle is prevented if certain necessary processes have not taken place
checkpoint controls
Mitosis will not happen if ______________has not been completed
DNA replication
There are several morphogenic aspects of the yeast cell cycle:
(name the 4)
1) Bud-site selection: in rich media haploids bud in axial pattern where diploids show polar budding
2) Polarity
3) Pattern
4) Rate of growth