Wound Repair Flashcards
Anytime you perform SURGERY you create a WOUND
* Leaves underlying tissue vulnerable to —
* ~ — million surgeries per year in the US
* Wound healing remains problematic →why and what can we do to lessen this?
infection
50
CAUSES OF INJURY
physical (7)
compromised blood flow
crushing
desiccation
incision
irradiation
overcooling
overheating
CAUSES OF INJURY
chemical (5)
agents with unphysiologic pH
agents with unphysiologic tonicity
proteases
vasoconstrictors
thrombogenic agents
EPITHELIALIZATION
* Noted with —
* Renewal of epithelium occurs within — after injury
* Occurs faster with — substrate over wound
abrasions
hours
moisten
Renewal of epithelium occurs within hours after injury
(2)
- Completion occurs at 24 – 48 hours
- Free edge of epithelium migrate until it contacts the opposite edge
- Free edge of epithelium migrate until it contacts the opposite edge
(2)
- Signal is terminated in CONTACT INHIBITION
- Occurs over wound bed but under scabbing/superficial blood clot
- Occurs faster with moisten substrate over wound
- Epithelium does cross over
desiccated surface
PHASES OF WOUND HEALING
* Inflammatory phase
* Day
* Fibroplastic phase
* Days
* Maturation/remodeling phase
*
1-6
4- 3 weeks
3 weeks – 1 year
INFLAMMATORY PHASE: DAYS 1- 5ISH
Also called LAG PHASE:
(4)
- No increase in wound strength
- Due to little collagen deposition
- FIBRIN principle material holding wound together
- Has little tensile strength
2 phases of the Inflammatory Phase
- Vascular
- Cellular
VASCULAR PHASE
* Vasoconstriction of disrupted vessels
(2)
- Coagulation (platelets and fibrin)
- Clot formation 5-10 minutes
Vasodilation
(3)
- Increases permeability to site to allow healing factors and cells to
reach injury site - Mediated by histamine and prostaglandins (E1 and E2) from WBCs
- Causes EDEMA (leak of cells and fluid to site of injury)
CELLULAR PHASE
* PMN (neutrophils) arrive within 24 hrs of injury
(4)
- Margination:
- Diapedesis:
- Degranulation:
- Macrophages continue clearance of debris
- Margination:
- PMNs stick to side of blood vessels
- Diapedesis:
- PMNs migrate through vessel walls
- Degranulation:
- PMNS releasing lysosomal enzymes to destroy bacteria/foreign
materials/necrotic tissue
FIBROPLASTIC PHASE: DAY 4 – 3 WEEKS
* — are the dominant cell
Fibroblasts
Fibroblasts are the dominant cell
* Deposits
ground substance and TROPOCOLLAGEN over fibrin lattice
* Ground substance contains mucopolysaccharides →cement collagen fibers together
Fibroblasts are the dominant cell
* Secretes
FIBRONECTIN
FIBRONECTIN
(3)
- Stabilize fibrin
- Assists in recognizing foreign material
- Chemotactic factor to aid recruitment of fibroblasts and macrophages
FIBROPLASTIC PHASE: DAY 4 – 3 WEEKS
* Angiogenesis occurs:
(2)
- Increase vascularity (from wound edges inward)
- Causes raised and red color of wound
FIBROPLASTIC PHASE: DAY 4 – 3 WEEKS
Superfluous fibrin strands removed by —
plasmin
FIBROPLASTIC PHASE: DAY 4 – 3 WEEKS
* Excessive collagen deposited in haphazard manner
(2)
- Increases tensile strength (~ 5-7 days after injury →timing for suture removal)
- 70%-80% tensile strength compared to uninjured tissue
REMODELING PHASE: 3 WEEKS TO 1 YEAR/INDEFINITE
* Increase in collagen ORGANIZATION AND STRENGTH
(3)
- Collagen oriented in direction to better resist tension
- Type III collagen replaced by Type I
- Excess collagen removed →scar softens
Wound strength never reaches above
80% - 85% of
uninjured tissue
* Peak tensile strength at 60 days
REMODELING PHASE: 3 WEEKS TO 1 YEAR/INDEFINITE
* Wound erythema decreases as
* Wound contraction occurs by
vascularity decreases to site
migration of wound edges
toward each other
FACTORS THAT IMPAIR WOUND HEALING
* Foreign material
(4)
- Dirt, wood, glass, suture, bacteria
- “Non-self” material causes chronic inflammation
- Decreases fibroplasia
- Bacteria proliferation causing infection
- Destroys host tissue with bacteria byproducts
- Non-bacteria causes a harbor for bacteria