Wound Management Flashcards
What regulates the process of wound healing
Soluble factors
What do abnormalities in wound healing cause?
Scarring
Fibrosis
What six things does wound healing involve?
Initial acute inflammatory response Parenchymal regeneration Re-epithelialisation and cell migration Proliferation of parenchyma and stromal cells Synthesis of ECM proteins Remodelling
What are the three classic stages of wound healing?
Inflammation - 48 hours after injury
New tissue formation - 2 to 10 days
Remodelling/maturation - 1 year or more
Describe the basic process of inflammation in wound healing
Hypoxic with a fibrin clot
Abundant bacteria, neutrophils and platelets
Describe the basic process of new tissue formation in wound healing
Surface scab
Most inflammatory cells moved away
New blood vessels predominate
Epithelial cells migrate under scab
Describe the basic process of remodelling in wound healing
Disorganized collagen made by fibroblasts that move into wound
Wound contracted near surface - widest part is deep
Re-epithelialized wound raised
What are the first four things to happen during wound healing?
Bleeding
Coagulation
Platelet activation
Complement activation
What two things care present or occurring during inflammation around day 1 of wound healing?
Granulocytes present
Phagocytosis occurring
What two things are present around day 3 of inflammation?
Macrophages
Cytokines
What four things are occurring around day 4-5 of new tissue formation?
Fibroplasia
Angiogenesis
Re-epithelialisation
ECM synthesis
What is occurring between days 30-100 in wound healing and what is increased and decreased?
ECM remodelling - increased tensile strength - decreased cellularity and vascularity
What cells are involved in coagulation?
Platelets
What cells are involved in inflammation?
Platelets
Macrophages
Neutrophils
What cells are involved in new tissue formation?
Macrophages Lymphocytes Fibroblasts Epithelial cells Endothelial cells
What cells are involved in remodelling?
Fibroblasts
What are the initial events in wound healing that lead up to coagulation?
Death of some epithelial and dermal cells
Damage to collagenous fibres in tissue
Small vessel rupture - increased vasodilation and permeability
Release of blood into wound and surrounding tissue
Coagulation
Formation of fibrin clot
What three things happen during coagulation?
Platelet deposition and aggregation
Platelets degranulate
PDGF, TGFb and fibronectin released
What are the key players in the inflammation stage of wound healing?
Monocytes
Macrophages
When are lymphocytes recruited in wound healing and what are they important in?
Recruited later
Important in early remodelling phase
What are the five roles of macrophages in wound healing?
Removal of wound debris Cell recruitment and activation Phagocytosis Angiogenesis Matrix synthesis regulation
Describe the process of re-epithelisation of the skin
Single keratinocyte layer migrates under fibrin clot
Travels from wound edges across wound to re-surface area
During and after this differentiation and stratification of neo-dermis occurs
What are the five roles of keratinocytes in skin healing?
Migration/proliferation ECM production Growth factor/cytokine production Angiogenesis Release of proteases
How does the fibrin clot help with re-epithelialisation and angiogenesis?
Secrete factors to promote re-epithelialisation
Allows endothelial cell migration into wound
Describe angiogenesis in wound healing
Begin as endothelial cell buds
Move towards wound space
Macrophages and keratinocytes provide stimuli
How does angiogenesis occur in other places?
Capillaries sprout from parent vessels Initiated by production of growth factors from nearby cells Endothelial cells produce proteases Cells migrate towards the growth factors Cells proliferate and divide Cells from tubes
Describe the early tubes formed by cells in angiogenesis
Leaky at first
Granulation tissue usually oedematous
Change in integrity when acquire support from surrounding cells
What occurs during fibroplasia in wound healing?
Fibroblasts migrate in and replicate
Synthesise and deposit ECM
Fibroblasts differentiate into myofibroblasts
Express contractile protein and effect wound closure
What are the four roles of fibroblasts in connective tissue formation and remodelling?
ECM production
GF and cytokine production
Angiogenesis
Protease release
When is granulation tissue established in wound healing?
Within 3-5 days post injury
Describe granulation tissue appearance
Pink, soft granular tissue
First appears beneath scab
Comprised of fibroblasts, thin walled capillaries and loose ECM
What is part of normal wound healing in the horse?
Exuberant granulation tissue - proud flesh
What is a normal and inevitable outcome of wound repair in mammals?
Scarring process
What is prolonged scarring called?
FIbrosis
What does scar formation rely on?
Rate of collagen synthesis vs. its rate of degradation
What extrinsic factors can modify wound healing?
Infection Nutrition Glucocorticoids Mechanical factors Poor blood flow Pathogens
How can the tissue type affect wound healing?
Total repair only possible when tissue contains labile cells
If only permanent cells only scarring can occur
Describe the basic process of re-epithelialisation
Blood forms clot blocking pathogen invasion
Inflammatory phase begins
Leukocyte influx
Endothelial cells migrate causing angiogenesis
Fibroblasts activated causing proliferation, migration and construction of granulation tissue
Epithelial cells migrate to from thin sheet and restore surface integrity
What are the six healing factors involved in wound healing?
EGF - epidermal/epithelial growth factor - mitogenic for epithelial cells and fibroblasts
PDGF - platelet derived growth factor - induces migration/proliferation of fibroblasts, vSMC and monocytes
FGF - fibroblast growth factor - induces fibroblast growth and angiogenesis
TGFbeta - transforming growth factor beta - promotes fibroblast migration/proliferation and ECM synthesis
VEGF - vascular endothelial growth factor - promotes angiogenesis
IL-1/TNFalpha - interleukin-1 and tumour necrosis factor - induces fibroblast proliferation
What are the seven regulators of wound healing?
Coagulation component Endogenous tissue factors Growth factors Interactions with ECM Cell-to-cell contacts and gap junctions Mechanical stimulation Oxidative stress
What are Esmarch’s five principles of wound management?
Non-introduction of anything harmful Tissue rest Wound drainage Avoidance of venous stasis Cleanliness
What are Halsted’s principles of surgery?
Haemostasis Aseptic technique Light touch Supply of blood preserved Tension-free closure Even tissue apposition Dead space obliterated
What are the ten major complications that can occur with wound healing?
Haemorrhage and haematoma Swelling and oedema Seroma Dehiscence Infection Tissue necrosis Scarring and contracture Draining tracts Exposed bone Non-healing wounds
How can acute marked haemorrhage affect wound healing?
Results in hypovolaemia
Affects wound healing
Potentiates wound infection
How can overzealous haemostasis affect wound healing?
Results in poor tissue viability
What ways can the presence of a haematoma influence wound healing?
Separates wound edges
Puts pressure on wound edges - necrosis and dehiscence
Prevents skin graft adherence to recipient bed
Barrier to migration of leukocytes and capillaries
Provides growth medium for bacteria
Describe conservative treatment for minor to moderate bleeding
Direct pressure Light bandage for up to 12 hours Restriction of movement of the body part Restriction of movement of the patient Investigate underlying coagulopathy Administration of IV fluids or bloods
What circumstances dictate surgical management of bleeding?
Arterial bleeding that is severe or non-responsive to conservative management
Dehiscence of the wound due to pressure
Development of compartment syndrome
Secondary infection of the haemoatoma
What can be the causes of oedema during wound healing?
Damage to regional blood vessels or lymphatics
Vascular occlusion - tight sutures/restrictive bandage
How can oedema affect wound healing?
Potentiates wound dehiscence
Delays wound healing by affecting wound vascularity
What wounds is post-operative oedema more marked in?
Regional mastectomy with tissue undermining
Reverse saphenous conduit flap
Free skin graft during plasmatic imbibition
Large distal limb wounds allowed to heal by second intention
Excision of lymph nodes
What should oedema be differentiated from?
Local infection
Cellulitis
What should regional oedema prompt an investigation of?
Things draining the region for pathological processes causing occlusion - veins, lymphatics and lymph nodes
What should generalised oedema prompt an investigation of?
Presence of hypoproteinaemia
Cardiac disease
What can moderate oedema benefit from?
Massage
Hot and cold packing
Physiotherapy
What may be needed for oedema treatment?
Removal of sutures contributing to vascular occlusion
Subsequent open wound management
Alternative closure plan
What is seroma?
Collection of serum and tissue fluid
Accumulates in a dead space and between tissue planes of a wound
How do seromas present?
Soft, fluctuant, non-painful swelling
Beneath skin incision
2-5 days after surgery
What factors contribute to the development of a seroma?
Inflammation Lymphatic injury Poor haemostasis Excessive tissue dissection Undermining creating dead space Traumatic surgical technique Poor tissue apposition Failure to manage dead space Constant motion at the surgical site Loose skin and tissue at the surgical site Use of suture material and mesh implants Repeated trauma to tissue from suture knots Release of vaso-active inflammatory mediators from mast cell tumour
How can fluid collecting between tissue layers delay wound healing?
Prevents tissue apposition
Prevents adherence of free skin graft to recipient bed
Puts pressure on wound edges increasing dehiscence risk
Interferes with blood supply to tissues
Inhibits influx of leukocytes potentiating wound infection
Which seromas require treatment?
Larger seromas
How should larger seromas be treated?
Drainage by aspiration
Management of dead space by bandage
Limit movement of animal and affected part
Drainage by indwelling drain
Removal of sutures and heal by second intention
What is dehiscence?
Breakdown of surgical wounds
What are the two main causes of wound dehiscence?
Excessive forces on the incision
Poor wound holding strength
When does most wound dehiscence occur?
3-5 days post surgery
What may be the initial signs of dehiscence?
Serosanguinous discharge from wound edges Non-painful subcutaneous wound swelling Necrosis of wound edges Extensive cutaneous bruising Serum below skin
What does the treatment of dehiscence depend upon?
Tissue layer that has suffered dehiscence
Cause of dehiscence
What should be done if dehiscence exposes vital structures to trauma?
Wound closed as soon as possible
How should dehiscence be treated if it occurs in the skin and subcutaneous tissue and is contaminated or infected?
Treat as an open wound
How can the risk of dehiscence due to wound infection be reduced?
Choosing delayed primary or secondary closure
Heal by second intention
What are the local signs of a wound infection?
Classical signs of inflammation
Serosanguinous to purulent discharge
Beyond what time does presence of inflammation, pyrexia or wound discharge suggest wound infection?
48 hours
What can be a strong indicator of wound infection?
Serosanguinous discharge from wound 3-5 days post surgery
What is the usual treatment for superficial wound infection?
Open wound management Remove sutures if necessary Debride devitalised tissue Lavage Drain
What may deeper wound infections require for treatment?
Wound exploration
Drain implantation
Samples taken for culture and sensitivity
What is delayed wound infection most commonly caused by?
Infection associated with implant presence - orthopaedics, non-absorbable mesh, non-absorbable suture material
What is another cause of delayed wound infection?
Failure of adequte debridement at first surgery
What is usually the cause of tissue necrosis?
Inadequate blood supply caused by trauma or surgery
How should necrotic tissue be removed?
Debridement of the wound
What are the consequences of not debriding the wound?
Increased infection risk Abscess formation Continued inflammation Additional metabolic load Delayed wound healing Poor cosmetic outcome
Where is excessive scarring not wanted?
Over joints
Near natural body orifices
How can scarring be reduced?
Meticulous atraumatic technique
Infection control
Early wound closure
What is wound contracture?
Loss of function of a body part as a result of excessive scarring
How can wound contracture be prevented?
Early recognition of wounds at risk - wounds near joints and body orifices, larger wounds left to heal by second intention
What can help to prevent contracture?
Early wound closure
What is needed once contracture has occurred?
Z-plasties Scar excision Partial myotomies Temporary splintage Physiotherapy Early return to normal therapy
When do adhesions develop?
When equilibrium between normal fibrin deposition and fibrinolysis is disrupted
What factors cause adhesion by disrupting the equilibrium?
Ischaemia
Haemorrhage
Foreign bodies
Infection
How can adhesion formation be reduced?
Atraumatic tissue handling
Keep tissues moist
Strict asepsis
What is a sinus?
Blind-ending tract that extends from an epithelial surface
What is a fistula?
Communicating tract that extends from one epithelial surface to another
What can draining tracts be associated with?
Large necrotic tissue pockets Resistant bacteria or fungi Underlying osteomyelitis or sequestrum Foreign bodies Foreign materials Neoplasia
What is required with draining tracts?
Surgical exploration
Debridement
Tissue biopsy for culture and histology
What should be done with each draining tract?
Identified
Excised
If not possible - explored, lavage, use open wound management or closure with drain
What is exposed bone most commonly associated with?
Distal limb wounds with gross tissue loss
Caused by shearing and degloving, or extensice necrosis from vascular injury or cellulitis
What may exposed bone be covered by?
Granulation tissue arising from viable periosteum