White/Purple

Question 1

This study started with a __instead of a candidate gene

Answer 1

candidate pathway- anthocyanin pigment synthesis

Question 2

Recombination events break up____

Answer 2

linkages

Question 3

Linkage continues to be broken up with more generation but for____it lasts a long time

Answer 3

close markers, like Hindlll site and position 229 codon with TG and GA-tall short peas

Question 4

Is there a loci where one allele(two copies) occurs in all white flowered individuals and the other allele in all purple individuals?

Answer 4

Yes
PEAPCF1 and CD72
both are RFLPs(restroom fragment length polymorphism)

Question 5

Photo on pg. 14 in lecture 4. what does A in between PEAPCF1 and CD72 mean?

Answer 5

A is the flower colour locus, they do not know hat it was but knew where is was by using the Medicago plant in comparison.

Question 6

What is a HLH. What is a diner?

Answer 6

A helix-loop-helix.
Dimer is two HLH proteins binding DNA

Question 7

From th Barrel clover with bHLH-A sequence they made a ___

Answer 7

degenerate PCR primers- which is mixtures of oligonucleotides that are used in PCR to amplifie unknown DNA sequences

This techniqued worked for the study

Question 8

What is a BAC and how was it found in bHLH-A

Answer 8

Bacteria Artificial Chromosomes
They designed new pea-specific primers to identify the bHLH-A positivr BAC from a pea genome library

Two BACS were sequenced

Question 9

What was difference in the bHLH-A in white flowers?

What makes most mRNA get destroyed?

Answer 9

8 extra base pairs at an end of exon Vl
this causes a frame shift

this causes the mRNA is have a PTC(premature stop codon)

cells called nonsensemediated decay destroyes mRNA with PTC

Question 10

What did they do to confirm that this is the mutation that causes white flowers?

explain photo on pg 9 lecture 5

Answer 10

They added a wt version of the bHLH-A to the white flowers with a gene gun… this resulted in purple flowers

GFP is inserted to show the purple pigment