Weeks 3-4 Flashcards
Streptomycin, tetracycline
inhibit prokaryotic translation via the 30S subunit.
Chloramphenicol, Erythromycin
inhibit prokaryotic translation via the 50S subunit.
Puromycin
prematurely prevents prokaryotic translation by accepting peptide bond
Diphtheria toxin
inhibits eukaryotic translation via inhibition of eEF-2 (translocation).
Ricin
inhibits eukaryotic translation via inhibition of peptidyl transferase activity.
Irinotecan, Topotecan
inhibit eukaryotic topoisomerase I
Doxorubicin, Etoposide
inhibit eukaryotic topoisomerase II
Ciprofloxacin
inhibits bacterial topoisomerase
RNAP I
transcribes rRNA in nucleoplasm.
RNAP II
transcribes mRNA, miRNA, and snRNA in nucleus.
RNAP III
transcribes tRNA in nucleoplasm.
Lamina
Attach to chromatin, integral membrane proteins (emerin), and NPCs, provide anchorage sites for chromosomes, which bind at centromeres and telomeres, bind TFs to regulate transcription.
CBs/Gems
sites of snoRNP and snRNP formation and maturation; also contain SMN (survival of motor neuron) proteins.
Speckles
sites of pre-mRNA processing.
Ran GTP
small G-protein that provides energy for NPC translocation; part of the Ran family; hydrolyzed by Ran-GAP.
Ran-GEF
an exchange factor that catalyzes the exchange of a GDP for a GTP on the Ran protein in the nucleus.
Smooth ER
synthesizes steroid hormones, plasma lipoproteins, detoxifies material (Ex: in hepatocytes), controls muscle relaxation/contraction, degrades glycogen, and functions in gluconeogenesis.
Rough ER
produces proteins destined for the ER, Golgi, lysosomes, endosomes, plasma membrane, and secretory vesicles; inner lumen = protein production and folding.
BiP
lumenal RER protein that aids in folding proteins.
Signal peptidase
cleaves N-terminus signal sequence as proteins enter the lumen.
