Week 9 - Gene Expression part 1

Question 1

Detection methods of mRNA?

Answer 1

Northern Blotting
ISH (In situ hybridisation)
RT-PCR/qPCR
Transcript profiling
Reporter plasmids/genes

Question 2

What is northern blotting?

Answer 2

It is the separation of RNA by gel electrophoresis, It detects when bands anneal to the probe, annealing of a band to a probe indicates gene expression.