Week 6 Flashcards

1
Q

What is a nutrient in the context of phytoplankton growth?

A

Nutrients are dissolved substances that provide essential elements (e.g., Nitrogen, Phosphorus, Iron) required for growth.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the difference between macronutrients and micronutrients?

A

Macronutrients: Needed in large quantities (e.g., Nitrogen, Phosphorus, Silicon). Micronutrients: Needed in trace amounts (e.g., Iron, Cobalt, Zinc).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What are the biological roles of Phosphorus, Nitrogen, and Iron?

A

Phosphorus: DNA, ATP, phospholipids. Nitrogen: Proteins, nucleic acids. Iron: Photosystems, enzymes like nitrogenase.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the main forms of Nitrogen available in the ocean?

A

Nitrate (NO₃⁻), Nitrite (NO₂⁻), Ammonium (NH₄⁺), Dissolved Organic Nitrogen (DON), Nitrogen gas (N₂, used by diazotrophs).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is the Redfield Ratio, and why is it important?

A

The stoichiometric ratio of elements in phytoplankton: 106 C : 16 N : 1 P. It reflects the balance of nutrients in the ocean and their influence on primary production.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How do nutrient concentrations vary between the surface and deep ocean?

A

Nutrients are depleted in the surface due to biological uptake and enriched in deeper waters due to organic matter remineralization.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What is the difference between ‘new’ and ‘regenerated’ nitrogen?

A

New Nitrogen: Nitrate or nitrogen from external sources (e.g., upwelling, N₂ fixation). Regenerated Nitrogen: Ammonium or urea recycled within the ecosystem.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What is the f-ratio, and how is it calculated?

A

The ratio of new nitrogen uptake to total nitrogen uptake: f = uptake of new N / (uptake of new N + regenerated N).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Why is nitrate more abundant below the thermocline?

A

Nitrate accumulates below the thermocline due to reduced biological uptake and organic matter remineralization.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is Liebig’s Law of the Minimum?

A

Growth is limited by the scarcest resource, even if other resources are abundant.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

How does the Michaelis-Menten equation describe nutrient uptake?

A

v = Vmax [S] / (KN + [S]) where: v = uptake rate, Vmax = max uptake rate, [S] = nutrient concentration, KN = nutrient concentration at half Vmax.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Which regions are classified as High Nutrient Low Chlorophyll (HNLC), and why?

A

HNLC regions (e.g., Southern Ocean, Equatorial Pacific) have abundant macronutrients but limited productivity due to iron deficiency.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Why is iron often a limiting nutrient?

A

Iron is insoluble in seawater and rapidly binds to particles, making it scarce in bioavailable forms.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What are key pieces of evidence supporting the iron limitation hypothesis?

A

Bottle Experiments: Adding iron increases productivity. In Situ Iron Fertilization: Experiments (e.g., SOIREE) show blooms after iron addition. Natural Iron Fertilization: Observations of blooms near iron-rich regions (e.g., volcanic ash inputs).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What was the outcome of the SOIREE experiment?

A

Adding iron to an HNLC region resulted in a phytoplankton bloom visible from space for over a month.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

How do phytoplankton adapt to low nutrient environments?

A

Smaller cells with a high surface area-to-volume ratio. Efficient nutrient uptake systems. Specialization for using different nutrient forms.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Why do small cells have an advantage in low nutrient environments?

A

They have a lower KN, meaning they can outcompete larger cells at low nutrient concentrations.

18
Q

What are the two main sources of nutrients to the euphotic zone?

A

Regeneration: Local recycling (e.g., ammonium from microbial degradation). External Inputs: Upwelling, N₂ fixation, terrestrial runoff.

19
Q

How do volcanic eruptions affect nutrient dynamics?

A

They can deposit iron-rich ash, stimulating phytoplankton blooms in HNLC regions (e.g., North Pacific after Kasatochi eruption).

20
Q

What are the typical nutrient concentrations in surface versus deep waters?

A

Surface: Low macronutrient levels due to biological uptake. Deep: High levels from remineralization of sinking organic matter.

21
Q

How do nutrient profiles vary in eutrophic, oligotrophic, and HNLC regions?

A

Eutrophic: High nutrients, high productivity. Oligotrophic: Low nutrients, low productivity. HNLC: High macronutrients, low productivity (iron-limited).

22
Q

What forms does phosphorus take in seawater, and what are their roles?

A

Dissolved Inorganic Phosphorus (DIP): Orthophosphate (PO₄³⁻). Dissolved Organic Phosphorus (DOP): Found in phospholipids, nucleic acids.

23
Q

What are the different oxidation states of iron in the ocean?

A

Iron(III) (Fe³⁺): Highly insoluble in seawater. Iron(II) (Fe²⁺): More soluble but rapidly oxidized. Organic Complexes: Most bioavailable iron is bound to organic ligands.

24
Q

How does remineralization affect the Redfield Ratio?

A

When organic matter is broken down, nutrients and CO₂ are released in the same 106 C : 16 N : 1 P ratio, while oxygen is consumed (-138 O₂).

25
How does the photosynthetic quotient (PQ) differ for nitrate versus ammonium-based growth?
Nitrate-based growth: PQ = 1.3 (higher due to oxidation during assimilation). Ammonium-based growth: PQ = 1.0 (no oxidation required).
26
How do diazotrophs contribute to the nitrogen cycle?
Diazotrophs (e.g., Trichodesmium) fix nitrogen gas (N₂) into bioavailable forms like ammonium, using the nitrogenase enzyme that requires iron and low oxygen.
27
What is the significance of nitrite (NO₂⁻) in low oxygen zones?
Nitrite accumulates due to: Bacterial oxidation of ammonium. Phytoplankton excretion.
28
What factors influence the saturation constant (KN) for nutrient uptake?
Cell size: Smaller cells have lower KN due to higher surface-area-to-volume ratios. Specific nutrient uptake systems: Adapted for efficiency in low-nutrient conditions.
29
What is the difference between Vmax and KN in nutrient uptake?
Vmax: Maximum rate of nutrient uptake. KN: Nutrient concentration at half of Vmax. Lower KN indicates better uptake efficiency.
30
What methods are used to assess nutrient limitation in ocean systems?
Collect water samples with microbes. Add limiting nutrients (e.g., nitrate, iron). Measure biological responses like growth or productivity.
31
What were the main findings of the SOIREE experiment?
Adding iron to the Southern Ocean (HNLC region) caused a significant phytoplankton bloom. The bloom was visible from space for over a month.
32
Why was the Iron Hypothesis controversial?
Critics argued that: Results from bottle experiments might not apply to natural ecosystems. Oceanic microbes behave differently in isolated conditions.
33
What natural events provide evidence for iron fertilization effects?
Volcanic eruptions (e.g., Kasatochi, Eyjafjallajökull) supply iron-rich ash, stimulating phytoplankton blooms. Natural iron inputs from sub-Antarctic islands support localized blooms.
34
What are specific adaptations of phytoplankton in oligotrophic waters?
Increased surface area-to-volume ratio for efficient nutrient uptake. Use of organic forms of nitrogen like urea or amino acids. Reduced chlorophyll content to conserve resources.
35
Why is the f-ratio higher in eutrophic regions?
Eutrophic regions receive large inputs of new nitrogen (e.g., nitrate from upwelling), supporting a higher proportion of new production.
36
How does the f-ratio differ between HNLC and oligotrophic regions?
HNLC: Moderate f-ratio (~30–50%), with iron limitation. Oligotrophic: Low f-ratio (3–20%), dominated by regenerated production.
37
What factors limit productivity in HNLC regions?
Iron limitation. Deep mixed layers below the critical depth. Grazing pressure.
38
Why is iron critical for phytoplankton in HNLC regions?
Iron is required for photosystems, nitrogenase, and other enzymes, but it is scarce due to low solubility and rapid particle scavenging.
39
How did the Kasatochi eruption affect phytoplankton?
The eruption deposited iron-rich ash into the North Pacific, triggering a large bloom in an HNLC region.
40
What are the ecosystem effects of iron fertilization?
Enhanced phytoplankton blooms can increase carbon export and support higher trophic levels, but effects are temporary and region-specific.
41
How does nutrient availability affect growth yield and rate?
Yield: Depends on the total availability of limiting nutrients. Rate: Influenced by nutrient concentration and uptake kinetics.
42
What is the relationship between mixed layer depth and productivity?
Shallow mixed layers allow phytoplankton to stay in light-rich zones, promoting productivity, while deep layers reduce light exposure and limit growth.