Week 5 & 6 Questions Flashcards
Which of the following viruses is a picornavirus?
a) Influenza
b) Rhinovirus
c) RSV
d) HIV
e) OC43
b.) rhinovirus
- Which of the following is a paramyxovirus?
a) Influenza
b) Rhinovirus
c) RSV
d) HIV
e) OC43
c.) RSV
- Which of the following is an orthomyxovirus?
a) Influenza
b) Rhinovirus
c) RSV
d) HIV
e) OC43
A.) Influenza
An unknown respiratory virus is causing disease and you want to have a living isolate to research. Which of the following techniques would you use?
a) Nucleic acid amplification
b) Whole genome sequencing
c) Serology
d) Virus culture
e) Histology
d.) virus culture
A diagnostic test involves testing for IgM. What is the test looking for?
a) Viral RNA
b) Viral DNA
c) Viral antigens
d) Changes in tissue morphology
e) Antibodies from recent infection
e.) Antibodies from recent infections
Primers that speciate rhinovirus genotypes are targeting which of the following?
a) Viral envelope genes
b) Non-structural genes
c) Capsid genes
d) 5’UTR
e) None of the above
c.) capsid genes
- In some infants, RSV will infect the lower respiratory tract and cause more serious disease. Which of the following are 3 presentations associated with this?
a) Runny nose
b) Bronchiolitis
c) Wheezing
d) Pneumonia
e) Skin rash
b.) Bronchiolitis
c.) Wheezing
d.) Pneumonia
In RSV pathology, which of the following combine to cause blocking of the airways? Multiple options apply.
a) Latent virus infection
b) Overabundant mucus secretion
c) Cell debris from dead epithelial cells
d) Influx of immune cells
e) All of the above
b.) overabundant mucus secretion
c.) Cell debris from dead epithelial
d.) Influx of immune cells
New pandemic strains of Influenza A can arise at any time. Which of the following allow this to occur? Multiple options apply.
a) Influenza A has a segmented genome
b) Different strains co-infecting the same cell
c) Ability of the virus to infect pigs and birds
d) All of the above
e) None of the above
d.) all of the above
- Which of the following are the wildlife reservoirs of influenza A virus?
a) Bats
b) Rodents
c) Birds
d) Fish
c.) birds
- Which of the following statements are correct about influenza A? Multiple options apply.
a) It is enveloped
b) It is a positive sense RNA virus
c) It carries its RdRp in the virion
d) Cleavage of the NA protein is required for fusion to occur
e) Cleavage of the HA protein is required for fusion to occur
A.) enveloped
c.) carriers RdRp
e.) cleave pf HA for fusion
- Which is the reservoir host of MERS?
a) Birds
b) Bats
c) Rodents
d) Pangolins
e) Camels
e.) camels
Which section of the SARS-CoV-2 spike protein binds to ACE2 receptor?
a) RBD, Subunit 1
b) Fusion peptide, Subunit 1
c) RBD, Subunit 2
d) Fusion peptide, Subunit 2
e) S1/S2 junction
a.) RBD subunit 1
(No RBD such thing as subunit 2)
You work in a path lab and a nasal swab comes in to be tested for major respiratory viruses.
A) How would you process this specimen?
B) Which viruses would you test for?
- Swab Processing: Place the nasal swab in RNA buffer (e.g., RTL buffer) to preserve viral RNA.
- RNA Extraction: Extract RNA from the buffered sample using an RNA extraction kit.
- RT-PCR: Perform RT-PCR on the extracted RNA to detect major respiratory viruses.
- Optional Viral Culture: If needed, inoculate cell cultures with the sample to observe for cytopathic effects, confirming viral presence.
b.) Rhinovirus, COVID-19, RSV, influenza A
Why do you think genes such as the nucleocapsid and the 5’UTR might be more conserved than genes such as those coding for the attachment or capsid proteins?
