Week 2 read 5.1-.5.14

Question 1

What is microbial growth

Answer 1

Increase in population

Question 2

Types to grow culture and what they are

Answer 2

1.Batch culture - Isolate in dish with 4 methods: Lag adapts to environment, Log cells grow, Stationary both rates the same Death, more cells die

2. Continuous growth -Open system - nutrients supplied and waste removed
3. Diauxic growth - uses two energy sources, uses up one THEN the other

Question 3

Name 3 main methods to monitor growth + adv/dis

Answer 3

Direct measurement - Count with chambers, quick but don’t know if cells are dead or alive

2. Viable count Spread on petri dish to incubate, accurate but time consuming
3. Dry weight, Precise but time consuming
4. Turbudity Change in light uses spectrophotometer, quick bet measures ALL cells

Question 4

Main factors in growth

Answer 4

Nutrients temp moisutre stress ph pressure atmosphere

Question 5

Necessary enzymes

Answer 5

SOD- super oxide dismutase, catalase

Question 6

Chemoautotrophs

Answer 6

makes energy

Question 7

Chemoheterotrophs

Answer 7

Finds energy

Question 8

Binary fission

Answer 8

Cell replicates: Chromosome replicated, cell elongates, wall in middle,splits

Question 9

Difference in binary fission and mitosis

Answer 9

BF in procaryotes/ bactieria Mitosis in eukaryotes

BF less complicated

BF in cytoplasm Mitosis in Nucleus

Question 10

Chemostat

Answer 10

Culture device to grow bacteria

Question 11

Cardinal temp

Answer 11

Max min and opt temperature for organism

Question 12

Extremophiles

Answer 12

high heat

Question 13

Thermophiles

Answer 13

high heat but lower

Question 14

Hyperthermophile

Answer 14

X treme

Question 15

Mesophiles

Answer 15

mid

Question 16

Pyscho

Answer 16

low