Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

MICROLAB > WEEK 2- COMMON LABORATORY APPARATUS AND EQUIPMENT USED IN MICROBIOLOGY LABORATORY > Flashcards

WEEK 2- COMMON LABORATORY APPARATUS AND EQUIPMENT USED IN MICROBIOLOGY LABORATORY Flashcards

1
Q

optical instrument that enlarges images of very small objects that cannot be seen
by the eyes.

A

MICROSCOPE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

A pressure chamber used to sterilize glass wares and other supplies by subjecting them to high pressure saturated steam

A

AUTOCLAVE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what is the saturated steam temperature needed in an autoclave to sterilize, and for how long?

A

121 C and 15-20 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

this is used to take microbiological cultures. They are swabbed onto or into the affected area, then wiped across the culture medium where the bacteria from the swab may grow

A

COTTON SWAB

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

used to grow and maintain microbiological cultures

A

INCUBATOR

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what are the other names for a petri dish?

A

petri plate or cell-culture dish

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

these are sterile plates used to contain media for bacterial growth

A

PETRI DISH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what are the other terms used for inoculation loop

A

SMEAR LOOP or MICROSTREAKER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

a simple tool used to retrieve an inoculum from a culture of microorganisms, also used to transfer microorganisms

A

INOCULATION LOOP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

this is where the specimen is placed

A

GLASS SLIDE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

this is placed over the sample (in a glass slide) in order to keep the sample off the objective lens

A

COVER SLIP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

this is a slender container usually made of glass or plastic. It can contain both liquid and solid sterilized media for culturing bacteria.

A

TEST TUBE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

usually made of plastic or glass, that is used to accurately measure volumes of liquid

A

GRADUATED CYLINDER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

it is a source of heat and can be used to sterilize an inoculation loop

A

ALCOHOL LAMP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

an instrument used to estimate a liquid culture’s density of microorganisms by counting individual colonies on an agar plate, slide or petri dish

A

COLONY COUNTER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

used to transport a measured volume of liquid, it can measure a smaller volume starting at 1 microliter

A

MICROPIPETTE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

used to measure and transfer small quantities of liquid

A

DROPPER

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

other term for biosafety cabinet

A

BIOLOGICAL SAFETY CABINET

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

this is an enclosed, ventilated laboratory workspace for safety working with materials contaminated with pathogens requiring a defined biosafety level

A

BIOSAFETY CABINET

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

one of the most important pieces of equipment used in the laboratory

A

MICROSCOPE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

most commonly used microscope

A

COMPOUND MICROSCOPE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

what are the two lens systems used in a compound microscope?

A

OBJECTIVE LENSES (OBJECTIVES) AND OCULAR LENSES (OCULARS, EYEPIECE)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

what type of image does an objective lens produce?

A

REAL IMAGE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

this is a magnified image of a specimen produced by the objectives

A

REAL IMAGE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

this magnifies the real image

A

OCULAR LENSES

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

THE MAGNIFIED IMAGE OF A REAL IMAGE IS WHAT?

A

RETINAL IMAGE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

the framework includes an ____ and a ____

A

ARM & BASE

28
Q

The base houses a _____ which illuminates the specimen

A

LIGHT SOURCE

29
Q

what is the light source usually made out of?

A

INCANDESCENT OR TUNGSTEN BULB

30
Q

a platform upon which the slide is placed for observation

A

STAGE

31
Q

a small _____ located in the center of the stage allows light rays from the light source to reach the specimen and small clips secure the slide

A

APERTURE

32
Q

what does the lens system consist of?

A

condenser, ocular lens and objective lenses

33
Q

focuses light on the specimen and fills the lens with light. It is a movable lens located beneath the stage and it is attached to the iris diaphragm which resembles the shutter of a camera in structure and function

A

CONDENSER

34
Q

controls the amount of light passing through the stage aperture to the specimen

A

IRIS DIAPHRAGM

35
Q

both the ____ and ___ regulate light from the light source

A

CONDENSER and DIAPHRAGM

36
Q

this is the lens located closest to the eye

A

OCULAR LENS

37
Q

what is the magnification of the ocular lens?

A

10x

38
Q

this includes the three objectives located on a rotating nosepiece

A

OBJECTIVE LENSES

39
Q

name the three objective lenses

A

LPO- Low power objective
HPO- high power objective
OIO- oil immersion objective

40
Q

this type of objective lens is used for all types of specimen and it allows for rapid location of the specimen

A

LPO- LOW POWER OBJECTIVE

41
Q

used to locate and focus bacterial smears before proceeding to observe with OIO

A

HPO- HIGH POWER OBJECTIVE

42
Q

other terms you can use interchangeably with HPO

A

HIGH DRY OBJECTIVE OR HIGH POWER

43
Q

this objective lens has the highest magnification

A

OIO- OIL IMMERSION OBJECTIVE

44
Q

this holds the objective lenses in place

A

REVOLVING NOSEPIECE

45
Q

this is the source of light

A

ILLUMINATOR

46
Q

the compound microscope provides ____, that is once the specimen is in focus with the LPO, it will almost bein in focus when changing to HPO or OIO

A

PARFOCAL VIEWING

47
Q

Rapid movement over a large distance to locate the specimen and used for initial focusing

A

COARSE ADJUSTMENT KNOB

48
Q

discreet movement over a short distance to locate the specimen, used to sharpen the image

A

FINE ADJUSTMENT KNOB

49
Q

the distance between the specimens and the bottom of the objective

A

WORKING DISTANCE

50
Q

TRUE OR FALSE: the greater the magnifying power of the objective, the shorter its working distance

A

TRUE

51
Q

this is the ability of the microscope to distinguish fine details

A

RESOLVING POWER

52
Q

____ can distinguish one dot from another as long as the dots are separated by a distance of at least 0.1mm

A

HUMAN EYE

53
Q

the ocular lens and objective lenses contribute to the ____ of the specimen

A

TOTAL MAGNIFICATION

54
Q

how do you obtain the total magnification?

A

multiply ocular magnification by magnification of the objective- 10x(ocular lens) 10x LPO- 40x HPO-OIO (100x)

55
Q

what is the magnification of LPO?

A

10X

56
Q

what is the total magnification of LPO

A

100x

57
Q

what is the magnification of HPO

A

40x

58
Q

what is the total magnification of the HPO

A

400x

59
Q

what is the magnification of OIO?

A

100x

60
Q

what is the total magnification of OIO?

A

1000x

61
Q

all non-glass surfaces can be cleaned using what?

A

SOFT CLOTH, GAUZE OR COMMERCIAL WIPES

62
Q

the lenses are cleaned with what?

A

SPECIAL PAPER OR LENS TISSUE

63
Q

WHAT LENS SHOULD YOU WIPE FIRST? AND THEN WHAT ORDER?

A

OCULAR LENS FIRST, THEN ALL THE OBJECTIVE LENSES LEAVING OIO LAST

64
Q

why do we clean the OIO last?

A

to avoid carrying oil to other lenses

65
Q

what can be done with the residual oil in OIO?

A

use small amount of lens cleaner to remove the excess residual immersion oil

66
Q

how should you transport the microscope?

A

hold it in an upright position using two hands, firmly hold the arm of the microscope with one hand while stabilizing the base with the other

MICROLAB (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions