Week 2 Charachteristics Of Micro Organisms Flashcards
Major Groups Of Microorganisms
(MOs)
Bacteria, viruses, Algae. Protozoa,fungi
Protozoa & Fungi
Micro organisms
- Protozoa - large group of one-cell organisms; do not have a rigid cell wall; found in freshwater, marine habitat & moist soil; small number are responsible for intestinal infections of humans and others invade the blood, lungs, liver, or brain
- Fungi - mushrooms, yeast, & mold; oral candidiasis is a common form of this group of mo’s found in the dental office.
Prokaryotes vs. Eukaryotes
1.Prokaryotes cells: Primitive cells
NO internal membrane
NO NUCLEUS
Eg.Bacteria
2.Eukaryote cells: Complex cells
With internal membranes.
HAVE A NUCLEUS
e.g. protozoa, algae, fungi, plants and animals.
Viruses are not in these 2
Prokaryotic Cell
Eukaryotic cell
Prokaryotic Cell
Flagella - long, thin extension that provides mobility. (propeller)
• Fimbriae, Pili - hair-like projections with fine short appendages that allow bacteria to adhere
• Cytoplasmic Membrane - regulates amount of molecules entering or leaving the cell.
• surrounds the cytoplasm; transport of nutrients; energy metabolism
- composed of lipids & proteins
- antimicrobial agents, hand washing agents, and mouth-rinses kill or inhibit the growth of bacteria by acting on the cytoplasmic membrane
• Mesosomes:
• Present mostly in gram-positive bacteria
• - are inward foldings of the cytoplasmic membrane
Prokaryotic contd
• Cytoplasm -contained within cytoplasmic
• membrane
• - aqueous solution which contains water, proteins, carbohydrates and inorganic salts.
Cell wall:
• - gives cell its shape; rigid; protection from mechanical damage
• Outer membrane of gram - negative bacteria:
• Contains endotoxin; transport of nutrients.
• When endotoxin is released from bacteria present in the body, it can cause damage to nearby body cells).
Capsule:
• Gelatinous covering produced by the cytoplasmic membrane
•Protection from drying, antiphagocytic; attachment to surfaces.
• The presence of capsules reduces the ability of WBC to surround, engulf, and destroy the bacterium through phagocytosis
Prokaryotic Cell, continued
Granules - function as an energy reserve
• Ribosomes - contain RNA and proteins (protein synthesis)
• Nucleoid - contains the DNA
- is embedded in the cytoplasm
• Endospores:
• Thick wall-walled structure;
• One of the most resistant forms of life against heat, drying, and chemicals.
• Defence mechanism/ protection against adverse conditions.
Plasmids- extra-chromosomal DNA
-give ability of bacteria to grow by being resistant to physical & chemical agents, and antibiotics
Eukaryotic Cell
•Cell Wall-maintains rigidity of the cell
• Flagella-provides mobility; each flagella contains hollow filaments called microtubules
Cilia-provides movement and permits faster mobility than flagella
Plasma (Cytoplasmic) Membrane-permits movement of molecules acros membrane by active and passive transport; endocytosis and exocytosis occurs at this site
• Endocytosis - taking in of matter
• Exocytosis - content released to exterior
Endoplasmic Reticulum
• Endoplasmic Reticulum-fluid-filled channels that synthesize lipids (smooth)and proteins (rough).
> Smooth ER-lacks ribosomal attachment and appears smooth
Rough ER contains ribosomes and appears rough.
Granules
• Granules-contain polysaccharides (starch) and lipids
Vacuoles
• Vacuoles- function to gather food.
Vesicles
Vesicles - surrounds proteins; that occur in endocytosis and exocytosis
Ribosomes
Ribosomes - produce protein, also is
- larger in eukaryotic cells than prokaryotic cell
Mitochondria
Mitochondria - function as the powerhouse
Chloroplasts
Function in photosynthesis
Golgi Complex
Golgi Complex - function to package protein; especially important for proteins destined for secretion
Lysosomes
Lysosomes - membranous sacs that contain digestive enzymes
Nucleus
Contains genetic DNA material
Characteristics Of Bacteria Cell
Size:
~ Smallest of the unicellular organisms (single celled)
~ Approx. 1-1.5 micrometers wide and 2-6 micrometers long.
~ Bacteria have a very rapid metabolic rate due to large surface
area to volume.
A staining procedure developed by Dr. Christian Gram differentiates bacteria into:
1. Gram -positive (blue /purple) have cell walls composed of a thick layer
- Gram -negative(pink / red) bacteria have cell walls with a thin layer
Cell Morphology (size and shape)
3 basic shapes:
- Spheres or cocci(singular coccus) - spherical shape.
- Rods or bacilli (singular bacillus) - rod shaped.
- Spirilla or spirochetes (singular spirillum) - spiral shape.
Bacterial growth is defined as an increase in cell numbers.
When these cells divide what’s that process called ?
Binary Fission
Each cell divides into 2 daughter cells
Common morphologies of Prokaryotic cells are :
:(A) Staphylococcus aureus (cocci)
(B) Bacillus subtilis (rods), and
(C) Treponema denticola (spirals) compared to
(D) human red blood cells. B subtilis has been mixed with the red blood cells in D for size comparison.
Five major chemical or physical conditions influence
growth of bacteria:
- Temperature
- Acidity: *pH of 5.5 - 8.5, optimal growth @ pH 7.0
- Nutrients
- Oxygen metabolism
- Water