Week 2 Flashcards
Ligase
Enzyme, joins pieces of dna
helicase
Enzyme, unwinds dna
Topoisomerase/gyrase
Enzyme, that relieves supercoiling
Primase
RNA polymerase that makes primer for replication
ssBP
Single stranded binding proteins binds to ssDNA and protect from tangling/reforming dsDNA
initiation
polymerisation starts
chain elongation
main phase of polymerisation
termination
polymerisation stop
okazaki fragments
dna fragments being generated on the lagging strand
Semi conservative DNA replication
DNA contains one older and one newly replicated strand
supercoiling
overwind DNA
replication fork
structure where DNA is being replicated on the lagging strands
promoter
Dna sequence near start site of transcription, bound by sygma factor which recruits RNA polymerase II complex
RNA polymerase II complex
Collection of proteins that make an RNA copy of DNA
Sigma factor
Common bacterial transcription factor