Week 12- molecular bio Flashcards
1
Q
what are restriction enzymes?
A
enzymes that cut DNA at a specific nucleotide sequence usually at palindromic sequences?
2
Q
what are palindromic sequences?
A
words that spell the same forward as they do backwards
3
Q
what is a cloning vector?
A
small piece of DNA taken from a virus or plasmid that can be maintained in an organism
a foreign DNA fragment that can be inserted for cloning purposes
4
Q
how can you over express your pcr product?
A
add it in to a vector
eg, human insulin was cloned and inserted into a plasmid and over expressed in cells
