Week 10 - Recombinant proteins

Question 1

What is mAb?

Answer 1

Monoclonal antibodies - used therapeutically.

Question 2

What is ATG?

Answer 2

Start codon.

Question 3

Some commonly used promoters and their sources?

Answer 3

P lac - E. Coli lactose operon
P trp - E. Coli tryptophan operon
P L - bacteriophage lambda
P tac (trp-lac) = a hybrid promoter engineered from the best parts of P lac and P trp. Stronger than either parent promoter.

Question 4

Glycosylation

Answer 4

Is the reaction in which a carbohydrate, i.e. a glycosyl donor, is attached to a hydroxyl or other functional group of another molecule (a glycosyl acceptor).