Week 1- CC Unit Of Measures

Question 1

highly purified chemical that can be measured directly to produce a sub- stance of exact known concentration and purity.

Question 2

Is a chemical that is the highest purity and cam
be measured directly to produce a substance
of exact known concentra琀椀on

Answer 2

Primarystandsrd

Question 3

NBS – (Na琀椀onal Bureau of standards) now is ____

Answer 3

NIST – (na琀椀onal ins琀椀tute of standard) developed Cer琀椀昀椀ed reference materials or Standard reference material (CRMs/SRMs)

Question 4

• American chemical society
• Establish the speci昀椀ca琀椀on of chemical in the
  analy琀椀cal grade category

Answer 4

ACS

Question 5

require that primary standard be at least 99.95% pure

Answer 5

IUPAC (Interna琀椀onal union of pure & applied chemistry)

Question 6

atomic weight standards

Answer 6

Grade A

Question 7

working standards >0.5% impurity

Answer 7

Grade D

Question 8

ultimate standards

Answer 8

Grade B

Question 9

primary standard > 0.002%
impurity

Answer 9

Grade C

Question 10

secondary derived from using grade C standards

Answer 10

Grade E

Question 11

Is substance of lower purity with concentrtaion
determined by comparison with primary
standard

o Depends not only its composi琀椀on, which
cannot be directly determined, but also on the analy琀椀cal reference method

Answer 11

 Secondary Standard

Question 12

water suitable for reagent and standard prepara琀椀on

Answer 12

Reagent Grade Water

Question 13

Procedures used in Reagent Grade Water

Answer 13

Distilled water
Deionized water
Reverse osmosis

Question 14

remove all organic material, can be dis琀椀lled more than once, can become more pure, a water solely puri昀椀ed by dis琀椀lla琀椀on.

Answer 14

Distilled water

Question 15

produced from dis琀椀lled water using either an anion or ca琀椀on exchange resin followed by replacement of the removed par琀椀cles with hydroxyl or hydrogen ions,
neither pure nor sterile

Answer 15

Deionized water

Question 16

uses pressure to force
water through a semipermeable membrane,
used for the pretreatment of water

Answer 16

Reverse osmosis (RO)

Question 17

For procedures that require maximum water purity such as:
 Prepara琀椀on of standard solu琀椀ons
 Ultramicro chemical solu琀椀on
 Measurement of monogramor
subnanogram concentra琀椀on
 Tissue or cell culture (or both)

Answer 17

Type I RLW

Question 18

o Use most in laboratory determina琀椀on, in
chemistry, hematology, microbiology,
immunology and other chemical lab tests

Answer 18

Type II RLW

Question 19

Used most in qualita琀椀ve measurement/examina琀椀ons, most procedure in urinalysis, parasitology and histology, washing glassware and procedures that do not require type I &II

o Carbon dioxide- free water, obtained by boiling type II water

o Used when C02, ammonia and oxygen may a昀昀ect analysis

Answer 19

Type III RLW

Question 20

clinical chemistry is involved in the analysis of biochemical byproducts found in biological 昀氀uids, such as serum, plasma, or urine,

Answer 20

Reference material

Question 21

Are an integral part of the instrument or be placed on the device for maintenance

Answer 21

Thermometers/ temperature

Question 22

Types of Thermometers/ temperature

Answer 22

1. Liquid in gas
2. Electronic thermometer/ thermistor probe
3. Digital thermometer

Question 23

All temperature devices must be calibrated to ascertain accuracy

Answer 23

Liquid in glass thermometers
Par琀椀al immersion thermometers
Total immersion thermometers

Question 24

– are used for refrigeration applications, and surface thermometers may be needed to check temperatures on flat surfaces, such as in an incubator or heating oven.

Answer 24

Total immersion thermometers

Question 25

are used for measuring temp in units such as hea琀椀ng blocks and water baths and should be immersed to proper height as indicated by the con琀椀nuous line etched on the thermometer stem

Answer 25

Par琀椀al immersion thermometers

Question 26

use a colored liquid (re or other colored material) or mercury encase in plas琀椀c or glass material w/ bulb at one end and a graduated stem. They usually measure temperatures between 20C and 400C

Answer 26

Liquid thermometer

Question 27

The accuracy of thermometer used to monitor the incubation tempt of an instrument should be verified every

Answer 27

6-12 mos do calibra琀椀ons

Question 28

Reference thermometer must certified by

Answer 28

NIST

Question 29

For the monitoring enzymatic rxn, thermometer should agree within

Answer 29

0.1 C w/ the NIST reference
thermometers

Question 30

 Thermometers used to check refrigerators &
freezers tempt should agree within ____ w/ the reference thermometer * If they differ more than 1.0C IT SHOULD BE DISCARDED

Answer 30

 Thermometers used to check refrigerators &
freezers tempt should agree within 1.0 C w/ the reference thermometer * If they di昀昀er more than 1.0C IT SHOULD BE DISCARDED

Question 31

It is imperative that preventive maintenance procedures be performed and the results recorded for all lab instrumentation

Answer 31

Preventive maintenance

Question 32

Preventive maintenance includes:

Answer 32

 This include maintenance of :
o Analytical balances
o Refrigerators o Freezers
o Centrifugses o Ovens
o Water baths
o Heating blocks o Thermometers o Pipettors
o Dilutors
o Automated analyzers and
o All lab equipment used for analyzing
specimens

Question 33

is performed at
scheduled tims such as per shift, daily, weekly, monthly or yearly

Answer 33

Preventive maintenance

Question 34

Most laboratory supplies must sa琀椀sfy certain tolerances of accuracy and fall into two classes of precision tolerance

Answer 34

(American Society for Tes琀椀ng and Materials)

Question 35

preferred for lab applica琀椀ons

Answer 35

Class A

Question 36

have twice the tolerance limits of
class A

Answer 36

Class B

Question 37

5 general types of glass

Answer 37

1. High thermal resistant glass
2. High silica glass
3. Glass with high resistance to alkalies
4. Low actinic glass
5. Standard flint glass