Week 1 Biosafety and waste management Flashcards
-Alertness at all times
-Stay informed
-Use common sense
-Listen to any instruction
First rule of self protection
Universal pprecautions (1987) Blood and bodu fluid precautions should be consistently used for all patients
CDC
Body fluids- semen, vaginal secretion, amniotic fluid, saliva, tears, csf, urine and breast milk, unfixed tissues, organs or blood slides
Potentially infectious materials
Minutes in heat sterilization with 250C
15 minutes
mg/L of ethylene oxide at 55-60C
450-500mg/L
Type of safety hazard
Source: Infectious agent
Biological hazard
Apply to blood and to others body fluids containing visible blood
Universal precautions
Emphasized the avoidance of contact with potentially infectious, moist fluid (except sweat) regardless of the presence or absence of blood
Body substance isolation
Applies to all body fluids, secretions, excretions, (except sweat) and tissue specimens.
Applies to non-intact and mucous membranes
Standard Precaution
Used when patients with known or suspected infection
Used when Standard precaution alone are insufficient
Transmission based precaution
_______ is the best way to break the chain of infection
Handwashing
Duration of proper handwashing
40-60 sec
Father of handwashing
Ignaz Semmelweis
duration of scrubbing
20 secs
Those not known to cause disease in healthy adults. Standards microbiological practies. No special equipment
BSL I
Common human pathogens. Autoclave must be available. Limited access. Most micro labs fall in this category
BSL II
Those that may cause serious or lethal disease via inhalation. Effective treatment available
BSL III
Those that pose high risk of life-threatening disease. May be transmitted by aerosis. No vaccine or therapy
BSL IV
Proper doning of PPE
Gown, Hairnet, Mask, Goggles, Gloves
Proper removal of PPE(Dofing)
Gloves, Goggles, Gown, Hairnet, Mask
Type of safety hazard
source: needles, lancets, broken glass
cuts, punctures, or blood-borne pathogen exposure
Sharp hazards
Type of safety hazard
source: Preservatives and reagent
Exposure to toxic carcinogens or caustic agents
Chemical Hazards
Used to remove particles that may be harmful to the employee who is working witg potentially infectious biologic specimens
Biosafety cabinet
Uses exhaust fan; no health hazard(Does not cause disease)
BSC CLASS 1
Uses blower and has vertical laminar flow; moderate potential hazard
BSC CLASS 2
self- contained ventilated system; lethal disease but with treatment
BSC CLASS 3
In NFPA what blue means
Health hazard
In NFPA what red means
Fire
What yellow means
Instability
White means
Specific hazard
always immediately wash with water for at least ______
15 mins
in chemical handling we shouldn put ____ to ____
Acid to water
daily exposure to blood and body fluids
Category I
Regular exposure to blood and body fluids
Category 2
No exposure to blood and body fluids
Category III
what should employers must offer to all personel in Category I and II
HBV
