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Wbc Count Flashcards

1
Q

Relative count for Nuetrophilic band

A

0-6 %

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2
Q

Relative count for Nuetrophilic Segmented

A

37-71%

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3
Q

Relative count for lymphocyte

A

24-44%

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4
Q

Relative count for monocyte

A

1-10%

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5
Q

Relative count for eosinophil

A

0-4%

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6
Q

Relative count for basophil

A

0-2%

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7
Q

Absolute count for neutrophilic band

A

0.0-0.7 x 10^9

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8
Q

Absolute count for nuetrophilic segmenter

A

1.5-7.4 x10^9/L

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9
Q

Absolute count for lymphocyte

A

1.0-4.4 x10^9/L

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10
Q

Absolute count for monocyte

A

0.1-1.0 x10 ^9/L

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11
Q

Absolute count for eosinophil

A

0.0-0.4 x 10^9/L

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12
Q

Absolute count for basophil

A

0.0-0.2 x10^9/L

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13
Q

Common diseases of Neutrophilia

A

Appendicitis
Bacterial infections Meningitis
Tonsillitis
Myelogenous leukemia

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14
Q

Common diseases of basophilia

A

Irradiation
Polycythemia vera
Splenectomy
Hemolytic anemia

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15
Q

Common disease of Lymphocytosis

A

Viral infections
Whooping cough
IM
Lymphocytic leukemia

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16
Q

Common disease for monocytosis

A

Brucellosis
Tuberculosis
Subacute bacterial
endocarditis
Typhus fever
Collagen disease
Hodgkin’s disease
Gaucher’s disease
Monocytic leukemia
Rickettsial infections

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17
Q

Common disease for eosinophilia

A

Allergies
Scarlet fever
Parasitic infections
Eosinophilic leukemia

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18
Q

can be estimated by comparing the cell to a red blood cell

A

cell size

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19
Q

take note of shape, size, structure, and color

A

nuclear characteristics

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20
Q

check for the amount and type of inclusions

A

cytoplasmic characteristics

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21
Q

Most commonly employed counting methods used in manual differential coun

A

Cross-sectional or crenellation method

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22
Q

There are several counting methods used in manual differential count, which include:

A
  1. Cross-sectional or crenellation method – most commonly employed
  2. Longitudinal or strip method
  3. Battlement method
  4. Meander method (two-field or four-field)
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23
Q

used for white cell count destroy or hemolyze all non-nucleated red blood cells.

A

diluting fluids

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24
Q

These cells, because they contain a nucleus, cannot be distinguished from the white blood cells.

A

nucleated red blood cells (NRBC)

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25
Q

Corrected WBC formula

A

Corrected WBC= Uncorrected / 100+ Number of NRBC per 100 wbc x 100

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26
Q

function to protect the body against invasion by foreign organisms or antigens. I

A

Leukocytes

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27
Q

Diluting fluid of WBC

A

3% Acetic acid

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28
Q

Buffer solution of WBC

A

Aged Distilled water

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29
Q

Washing time is too long

A

slides that are too pink

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30
Q

Staining time is too short

A

Too pink

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31
Q

pH of stain is too low

A

Too pink

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32
Q

buffers too acidic

A

Too pink

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33
Q

Staining time is too long

A

Slides that are too blue

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34
Q

Burffering and/or washing time is too short

A

Slides that are too blue

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35
Q

pH stain is too high

A

Slides that are too blue

36
Q

Buffer is too alkaline/basic

A

Slides that are too blue

37
Q

stains most commonly used for the routine microscopic examination of blood are

A

Polychromatic stain

38
Q

Basic dye that gives blue color

A

Methylene blue

39
Q

Acidic dye that gives a red-orange color

40
Q

Methyl alcohol (methanol)

41
Q

Carefully place a small drop of blood at approximately

42
Q

Angle of smear preparation

A

30- to 45-degree angle

43
Q

Causes of Extremely thick smears

A

Too large drop of blood
Too fast in spreading
Too high angle of the speader slide

44
Q

Excess plasma causes nucleated cells to shrink and stain intensely, making identification difficult.

A

Extremely thick smear

45
Q

Red cells form more rouleaux in thick areas and cannot be evaluated

A

Extremely thick smear

46
Q

Causes of Extremely thin smears

A

Too small drop of blood
Too slow in spreading
Too low angle of the spreader slide

47
Q

Smudge cells are increased and red cells become artificially spheroid with a distorted shape.

A

Extremely thin smears

48
Q

There is a tendency for more nucleated cells to be carried out to the edges of smears that have been made with too slow a stroke and this affects the accuracy of the differential count

A

Extremely thin smears

49
Q

Causes Gritty appearance of feathered areas (tail

A

Accumulation of nucleated cells
Accumulation of leukocytes at the tail end
Using only a part of the drop of blood
Rough edge or dirty spreader slide

50
Q

Attributable to a large number of leukocytes in the sample

A

Accumulation of nucleated cells

51
Q

May be seen when using heparin

A

Accumulation of leukocytes at the tail end

52
Q

peripheral smear evaluation is the capstone on a panel of test

A

CBC or hemogra,

53
Q

Thoma pipet mark WBC

54
Q

Draw the diluting fluid into the pipet slowly until the mixture reaches the

55
Q

How many Wbc tertiary squares

56
Q

Rbc tertiary squares

57
Q

Monocytes and lymphocytes are

A

Agranulocytes

58
Q

What are the mature granulocytes

A

Neutrophil
Eosinophil
Basophil

59
Q

These three cell lines are distinguishable from each other by the presence of specific granules that appear in the on what stage?

A

Myelocyte stage

60
Q

Leukocytes can be classified into two groups depending on the presence (or absence) of

A

Cytoplasmic granules

61
Q

leukocytes possessing specific granules in their cytoplasm

A

Granulocytes

62
Q

Do not possess uch granules

A

Agranulocytes

63
Q

The monocyte arises from the same bipotential stem cell as the neutrophil

64
Q

The precursor to the monocyte

65
Q

Unlike the other white blood cells in the peripheral blood, it is considered to be immature cell

66
Q

Lymphocytes are produced by the

A

Lymph nodes
Spleen
Thymus
Bone marrow

67
Q

The lymphocytic series is composed of the

A

Lymphoblast
Prolymphocyte
Mature lymphocyte

68
Q

vital to the immune system and function in the production of circulating antibodies and in the expression of cellular immunity.

A

Lymphocytes

69
Q

Smear that is most convenient, and most commonly used method, recommend by CLSI for WBC differential counting

A

Wedge smear

70
Q

angle of the spreader must be lower to 250

A

Polyctemia vera (increased Hct)

71
Q

angle of the spread must be higher

A

Anemia (decreased Hct)

72
Q

CHARACTERISTIC OF AN IDEAL BLOOD SMEAR (Wedge method)

A

2/3 to 3⁄4 the length of the film slide

Finger-shaped

73
Q

For best results, blood smears should be stained

A

2 to 3 hours of specimen collection

74
Q

Indicator of the average/mean volume of RBCs.

A

Mean cell volume

75
Q

Mcv formula

A

MCV = Hctx10/Rbc count

76
Q

Reference range of mcv

77
Q

Indicator of the average weight of hemoglobin and RBCcount

78
Q

MCH FORMULA

A

MCH= 𝐻𝐸𝑀𝑂𝐺𝐿𝑂𝐵𝐼𝑁(𝑔/𝑑𝑙) 𝑥10/ RBC COUNT

79
Q

It is a measure of the average concentration of hemoglobin and hematocrit values

80
Q

MCHC FORMULA

A

MCHC = 𝐻𝐸𝑀𝑂𝐺𝐿𝑂𝐵𝐼𝑁(𝑔/𝑑𝑙) 𝑥100 /𝐻𝐸𝑀𝐴𝑇𝑂𝐶𝑅𝐼𝑇 (%)

81
Q

RR OF MCHC

82
Q

RR OF MCH

83
Q

RBC indices that express the mass of hemoglobin

84
Q

RBC indices that reflects RBC Diameter in blood

85
Q

Fourt rbc indices

86
Q

Asses the degree of anisocytosis

87
Q

Used in conjunction with MCV to classify anemia

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