Wbc Count Flashcards
Relative count for Nuetrophilic band
0-6 %
Relative count for Nuetrophilic Segmented
37-71%
Relative count for lymphocyte
24-44%
Relative count for monocyte
1-10%
Relative count for eosinophil
0-4%
Relative count for basophil
0-2%
Absolute count for neutrophilic band
0.0-0.7 x 10^9
Absolute count for nuetrophilic segmenter
1.5-7.4 x10^9/L
Absolute count for lymphocyte
1.0-4.4 x10^9/L
Absolute count for monocyte
0.1-1.0 x10 ^9/L
Absolute count for eosinophil
0.0-0.4 x 10^9/L
Absolute count for basophil
0.0-0.2 x10^9/L
Common diseases of Neutrophilia
Appendicitis
Bacterial infections Meningitis
Tonsillitis
Myelogenous leukemia
Common diseases of basophilia
Irradiation
Polycythemia vera
Splenectomy
Hemolytic anemia
Common disease of Lymphocytosis
Viral infections
Whooping cough
IM
Lymphocytic leukemia
Common disease for monocytosis
Brucellosis
Tuberculosis
Subacute bacterial
endocarditis
Typhus fever
Collagen disease
Hodgkin’s disease
Gaucher’s disease
Monocytic leukemia
Rickettsial infections
Common disease for eosinophilia
Allergies
Scarlet fever
Parasitic infections
Eosinophilic leukemia
can be estimated by comparing the cell to a red blood cell
cell size
take note of shape, size, structure, and color
nuclear characteristics
check for the amount and type of inclusions
cytoplasmic characteristics
Most commonly employed counting methods used in manual differential coun
Cross-sectional or crenellation method
There are several counting methods used in manual differential count, which include:
- Cross-sectional or crenellation method – most commonly employed
- Longitudinal or strip method
- Battlement method
- Meander method (two-field or four-field)
used for white cell count destroy or hemolyze all non-nucleated red blood cells.
diluting fluids
These cells, because they contain a nucleus, cannot be distinguished from the white blood cells.
nucleated red blood cells (NRBC)
Corrected WBC formula
Corrected WBC= Uncorrected / 100+ Number of NRBC per 100 wbc x 100
function to protect the body against invasion by foreign organisms or antigens. I
Leukocytes
Diluting fluid of WBC
3% Acetic acid
Buffer solution of WBC
Aged Distilled water
Washing time is too long
slides that are too pink
Staining time is too short
Too pink
pH of stain is too low
Too pink
buffers too acidic
Too pink
Staining time is too long
Slides that are too blue
Burffering and/or washing time is too short
Slides that are too blue