Wbc Count Flashcards by Alessandra May Limbo

Relative count for Nuetrophilic band

0-6 %

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Relative count for Nuetrophilic Segmented

37-71%

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Relative count for lymphocyte

24-44%

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Relative count for monocyte

1-10%

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Relative count for eosinophil

0-4%

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Relative count for basophil

0-2%

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Absolute count for neutrophilic band

0.0-0.7 x 10^9

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Absolute count for nuetrophilic segmenter

1.5-7.4 x10^9/L

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Absolute count for lymphocyte

1.0-4.4 x10^9/L

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Absolute count for monocyte

0.1-1.0 x10 ^9/L

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Absolute count for eosinophil

0.0-0.4 x 10^9/L

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Absolute count for basophil

0.0-0.2 x10^9/L

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Common diseases of Neutrophilia

Appendicitis
Bacterial infections Meningitis
Tonsillitis
Myelogenous leukemia

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Common diseases of basophilia

Irradiation
Polycythemia vera
Splenectomy
Hemolytic anemia

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Common disease of Lymphocytosis

Viral infections
Whooping cough
IM
Lymphocytic leukemia

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Common disease for monocytosis

Brucellosis
Tuberculosis
Subacute bacterial
endocarditis
Typhus fever
Collagen disease
Hodgkin’s disease
Gaucher’s disease
Monocytic leukemia
Rickettsial infections

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Common disease for eosinophilia

Allergies
Scarlet fever
Parasitic infections
Eosinophilic leukemia

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can be estimated by comparing the cell to a red blood cell

cell size

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take note of shape, size, structure, and color

nuclear characteristics

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check for the amount and type of inclusions

cytoplasmic characteristics

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Most commonly employed counting methods used in manual differential coun

Cross-sectional or crenellation method

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There are several counting methods used in manual differential count, which include:

Cross-sectional or crenellation method – most commonly employed
Longitudinal or strip method
Battlement method
Meander method (two-field or four-field)

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used for white cell count destroy or hemolyze all non-nucleated red blood cells.

diluting fluids

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These cells, because they contain a nucleus, cannot be distinguished from the white blood cells.

nucleated red blood cells (NRBC)

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Corrected WBC formula

Corrected WBC= Uncorrected / 100+ Number of NRBC per 100 wbc x 100

function to protect the body against invasion by foreign organisms or antigens. I

Leukocytes

Diluting fluid of WBC

3% Acetic acid

Buffer solution of WBC

Aged Distilled water

Washing time is too long

slides that are too pink

Staining time is too short

Too pink

pH of stain is too low

Too pink

buffers too acidic

Too pink

Staining time is too long

Slides that are too blue

Burffering and/or washing time is too short

Slides that are too blue

pH stain is too high

Slides that are too blue

Buffer is too alkaline/basic

Slides that are too blue

stains most commonly used for the routine microscopic examination of blood are

Polychromatic stain

Basic dye that gives blue color

Methylene blue

Acidic dye that gives a red-orange color

Eosin

Methyl alcohol (methanol)

Fixative

Carefully place a small drop of blood at approximately

1 cm

Angle of smear preparation

30- to 45-degree angle

Causes of Extremely thick smears

Too large drop of blood Too fast in spreading Too high angle of the speader slide

Excess plasma causes nucleated cells to shrink and stain intensely, making identification difficult.

Extremely thick smear

Red cells form more rouleaux in thick areas and cannot be evaluated

Extremely thick smear

Causes of Extremely thin smears

Too small drop of blood Too slow in spreading Too low angle of the spreader slide

Smudge cells are increased and red cells become artificially spheroid with a distorted shape.

Extremely thin smears

There is a tendency for more nucleated cells to be carried out to the edges of smears that have been made with too slow a stroke and this affects the accuracy of the differential count

Extremely thin smears

Causes Gritty appearance of feathered areas (tail

Accumulation of nucleated cells Accumulation of leukocytes at the tail end Using only a part of the drop of blood Rough edge or dirty spreader slide

Attributable to a large number of leukocytes in the sample

Accumulation of nucleated cells

May be seen when using heparin

Accumulation of leukocytes at the tail end

peripheral smear evaluation is the capstone on a panel of test

CBC or hemogra,

Thoma pipet mark WBC

0.5

Draw the diluting fluid into the pipet slowly until the mixture reaches the

How many Wbc tertiary squares

Rbc tertiary squares

25 center

Monocytes and lymphocytes are

Agranulocytes

What are the mature granulocytes

Neutrophil Eosinophil Basophil

These three cell lines are distinguishable from each other by the presence of specific granules that appear in the on what stage?

Myelocyte stage

Leukocytes can be classified into two groups depending on the presence (or absence) of

Cytoplasmic granules

leukocytes possessing specific granules in their cytoplasm

Granulocytes

Do not possess uch granules

Agranulocytes

The monocyte arises from the same bipotential stem cell as the neutrophil

CFU-GM

The precursor to the monocyte

Monoblast

Unlike the other white blood cells in the peripheral blood, it is considered to be immature cell

Monocyte

Lymphocytes are produced by the

Lymph nodes Spleen Thymus Bone marrow

The lymphocytic series is composed of the

Lymphoblast Prolymphocyte Mature lymphocyte

vital to the immune system and function in the production of circulating antibodies and in the expression of cellular immunity.

Lymphocytes

Smear that is most convenient, and most commonly used method, recommend by CLSI for WBC differential counting

Wedge smear

angle of the spreader must be lower to 250

Polyctemia vera (increased Hct)

angle of the spread must be higher

Anemia (decreased Hct)

CHARACTERISTIC OF AN IDEAL BLOOD SMEAR (Wedge method)

2/3 to 3⁄4 the length of the film slide Finger-shaped

For best results, blood smears should be stained

2 to 3 hours of specimen collection

Indicator of the average/mean volume of RBCs.

Mean cell volume

Mcv formula

MCV = Hctx10/Rbc count

Reference range of mcv

80-100 fL

Indicator of the average weight of hemoglobin and RBCcount

MCH

MCH FORMULA

MCH= 𝐻𝐸𝑀𝑂𝐺𝐿𝑂𝐵𝐼𝑁(𝑔/𝑑𝑙) 𝑥10/ RBC COUNT

It is a measure of the average concentration of hemoglobin and hematocrit values

MCHC

MCHC FORMULA

MCHC = 𝐻𝐸𝑀𝑂𝐺𝐿𝑂𝐵𝐼𝑁(𝑔/𝑑𝑙) 𝑥100 /𝐻𝐸𝑀𝐴𝑇𝑂𝐶𝑅𝐼𝑇 (%)

RR OF MCHC

3w-36g/dL

RR OF MCH

26-32 pg

RBC indices that express the mass of hemoglobin

MCH

RBC indices that reflects RBC Diameter in blood

MCV

Fourt rbc indices

RDW

Asses the degree of anisocytosis

RDW

Used in conjunction with MCV to classify anemia

RDW