WBC

Question 1

Total WBC count

Answer 1

4k-11k/mm3 of blood

Question 2

What is 1 cubic mm in terms of volume?

Answer 2

0.001 ml

Question 3

Leishman’s stain contains?

Answer 3

EOSIN and METHYLENE BLUE in acetone-free methyl alcohol

Question 4

Which structures absorb basophilic stains?

Answer 4

Anionic or acidic structures

Question 5

Methylene blue is___________(acidic/basic) which gives ___________ colour.

Answer 5

Basic stain
Blue/Purple colour

Question 6

Eosin is___________(acidic/basic) which gives ___________ colour.

Answer 6

Acidic stain
Pink/Orange-red colour

Question 7

What colour does neutrophilic structures show upon staining?

Answer 7

Lilac

Question 8

What is the function of acetone-free methyl alcohol in Leishman’s stain?

Answer 8

Fixative and preservative

Question 9

What are the sizes of the different leukocytes?

Answer 9

Basophils: 8-10 µm Small lymphocytes: 6-8 µm Neutrophils: 10-14 µm Large lymphocytes: 10-15 µm Eosinophils: 12-15 µm
Monocytes: 15-20 µm

Question 10

What do phosphatases do?

Answer 10

Phosphatases are the hydrolytic enzymes that (remove phosphate group from proteins) cleave the ester bond between the phosphate group and the organic residue of the organic phosphates. Based on the optimum pH for the activity, phosphatases are of two kinds: acid and alkaline.