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Biochemistry > Water, pH + Buffers > Flashcards

Water, pH + Buffers Flashcards

1
Q

What do strong acids do in water?

What is produced?

A

They completely dissociate!

A proton

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2
Q

A proton acceptor is called a…

A

A base

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3
Q

A proton donator is called a…

A

An acid

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4
Q

What is physiological ph?

A

7.4 (within 0.3 either side of that, + or -)

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5
Q

pH is a measure of what?

A

Protons

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6
Q

What are the units of the concentration of protons in pH?

A

Molar - M

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7
Q

What solution can resist changes to pH?

A

A buffer

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8
Q

At what point does a conjugate base EQUAL a conjugate acid?

A

pKa

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9
Q

What is the pKa when the buffering region is 4.5 to 6.5?

A

5.5 (buffering region is pKa + or - 1)

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10
Q

pH of a lysosome is…

A

About 4.5

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11
Q

The hydrogen phosphate system controls the pH of what?

A

Cellular pH!

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12
Q

The bicarbonate system controls the pH of…

A

Blood + Gastric Acid

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13
Q

What % of the brain is water?

A

75%

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14
Q

Are hydrogen bonds unique to water?

A

NO - there are many variations!

E.g. Nitrogen can be involved or any other electronegative atom.

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15
Q

A reaction where 2 molecular fragments are joined and H20 is PRODUCED is called a …

A

Condensation Reaction

Hydrolysis is when water is LOST

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16
Q

To be soluble in water, the solute must be…

A

Polar or charged! (Hydrophilic)

17
Q

What is the predominant form of water at room temperature?

A

H2O

18
Q

pH of pure water is 7.

In the lab, distilled water is often pH 5.5-6.5

Why?

A

It reacts with CO2 in the air as we are NOT in a vacuum. And this forms carbonic acid.

19
Q

Stomach acid is composed of 0.05 - 0.1 M HCL = pH 1-1.3

The pH of stomach (gastric acid) is pH 1.5-3.5. Why?

A

There are cells within our stomach (goblet cells) that produce bicarbonate (a base) which acts as a BUFFER.
This ensures that the stomach does NOT become too acidic!

20
Q

In a highly basic solution, pH = 13, the dominant form of glycine is:

A

NH2 - CH3+ - COO-

21
Q

How much glucose (in grams) do you need to make 100mL of a 1M solution. The FW of glucose is 180g mol-1.

A 
W = 180g
V = 0.1L (convert the 100mL to litres!)
M = 1M

Multiply them all together:
180 x 0.1 x 1 = 18g!

Biochemistry (9 decks)

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