Vwf

Question 1

What does vwf:cba detect?

Answer 1

ability of vwf to bind to collagen

measures preferentially the High Molecular Weight [HMW] multimers of VWF

Question 2

Method CBA

Answer 2

Elisa- wells coated w Human collagen type III
Plasma added
Washed
HRP added to deft ab present by binding causing colour change
Amy colour proportional,to amt vwf bound to collagen

Question 3

Dx of type 2n requires?

Answer 3

Pedigree analysis
Genetics
FVIII binding assa
Measure both fviii and vwf activity

Question 4

How do,you ensure vwf assays remain précis

Answer 4

Use of controls w known vwf concentration to gen curves

Question 5

Other DDx for mucosal bleeding

Answer 5

Acquired VWD, 
platelet-type VWD, 
platelet function defects, 
vascular malformations such as hereditary hemorrhagic telangiectasias
 connective tissue disorders

Question 6

Which r on plts does vwf bind to

Answer 6

to platelet glycoprotein Iba

Question 7

Pt related rf for inhibitor development

Answer 7

Race
Fhx
FVIII mutation
MHC class
Polymorphism of immune response genes- il 10, TNF, CTLA4

Question 8

How does fviii work

Answer 8

Travels bound to Vwf and when act by fvii will activate fix

Question 9

How is FVIII cleared

Answer 9

By fv and fix

Short half life

Question 10

Riusk factor for F5 inhibitors

Answer 10

by exposure to bovine protein (i.e. bovine thrombin preparations)
Antibodies are generated as an immune response to bovine factor V which then cross-reacts with
recent surgery,
drug exposure (e.g. aminoglycosides, B-lactam antibiotics),
infections,
blood transfusions,
autoimmune disorders,
malignancy
pregnancy