Vwf Flashcards

1
Q

What does vwf:cba detect?

A

ability of vwf to bind to collagen

measures preferentially the High Molecular Weight [HMW] multimers of VWF

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2
Q

Method CBA

A

Elisa- wells coated w Human collagen type III
Plasma added
Washed
HRP added to deft ab present by binding causing colour change
Amy colour proportional,to amt vwf bound to collagen

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3
Q

Dx of type 2n requires?

A

Pedigree analysis
Genetics
FVIII binding assa
Measure both fviii and vwf activity

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4
Q

How do,you ensure vwf assays remain précis

A

Use of controls w known vwf concentration to gen curves

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5
Q

Other DDx for mucosal bleeding

A
Acquired VWD, 
platelet-type VWD, 
platelet function defects, 
vascular malformations such as hereditary hemorrhagic telangiectasias
 connective tissue disorders
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6
Q

Which r on plts does vwf bind to

A

to platelet glycoprotein Iba

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7
Q

Pt related rf for inhibitor development

A
Race
Fhx
FVIII mutation
MHC class
Polymorphism of immune response genes- il 10, TNF, CTLA4
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8
Q

How does fviii work

A

Travels bound to Vwf and when act by fvii will activate fix

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9
Q

How is FVIII cleared

A

By fv and fix

Short half life

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10
Q

Riusk factor for F5 inhibitors

A

by exposure to bovine protein (i.e. bovine thrombin preparations)
Antibodies are generated as an immune response to bovine factor V which then cross-reacts with
recent surgery,
drug exposure (e.g. aminoglycosides, B-lactam antibiotics),
infections,
blood transfusions,
autoimmune disorders,
malignancy
pregnancy

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