Vocab/Parasite Info Flashcards

1
Q

Fascioliasis

A
  • *Fasciola Hepatica *(“live fluke”)
  • Chronic
    • Cattle, sheep
      • sheep get bottle jaw
    • anemia
    • eggs in feces
    • hepatic fibrosis
  • Acute
    • Sheep
      • doesn’t acquire immunity
    • flukes accumulate → severe disease
      • Traumatic hepatitis
      • Death (intra-abdominal hemorrhage)
    • No eggs in feces (prepatent)
      • Can’t diagnose w/ fecal sedimentation
  • Subacute
    • less sudden onset of anemia, hypoproteinemia
    • Eggs in feces
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
1
Q

*Dirofilaria immitis *(heartworm) Diagnostics

A
  • History, clinical signs
  • Detection of Ag
    • SNAP, Witness, Solostep, Dirocheck, Snap 4DX
    • Test annually
    • 5-7 mos after infection
  • Detection of circulating Mff
  • Imaging
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Order Anoplura

A

Sucking Lice

  • Phylum arthropoda
  • Class insecta
  • Head is narrower than thorax
  • Feed on blood
  • Stenoxenous - mammals only
    • Some are even region specific on animal (ex. head, body, pubic area)
  • Simple metomorphosis
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Trichinella spiralis Life Cycle

A

Female deposits larvae in intestinal wall → larvae enter lymphatics → Thoracic duct → Heart → Lungs → Heart → Systemic circulation → lavae encyst in striated muscle (encapsulated by nurse cell) → excyst upon digestion by different host → mature in small intestine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Ag test: Negative

Microfilariae test: Positive

(*Dirofilaria immitis *aka heartworm Dx)

A
  • Mff are those of another species, low in number
  • Mff acquired transplacentally, low in number (young dogs, rare)
  • Adult worms removed/died, Mff persist
  • Test contamination
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Phylum Apicomplexa

A
  • Kingdom protista
  • Distinct nucleus
  • Subpellicular tubules (movement)
  • Apical complex
  • Oocytes (NOT eggs!)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Cytauxzoon felis

A
  • Kingdom protista
  • Phylum apicomplexa
  • Order piroplasmida
  • DH: domestic cats (esp. feral, farm cats)
  • IH: *Dermacentor variabilis *(dog tick), *Amblyomma americanum *(lone star tick)
  • Reservoir host: bobcat
  • Target organ: macrophages and RBCs
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Class 3 Heartworm Disease

A

Moderate to severe

  • Cardiac cachexia (wasting)
  • Constant fatigue
  • Persistent cough
  • Dyspnea
  • +/- signs of R sided heart failure
    • acites
    • jugular vein distention (JVD)
  • Significant anemia (
  • Proteinuria (>2+)
  • Rads
    • RV +/- RA enlargement
    • PA enlargement
  • Pulmonary thromboembolism (PTE)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

*Eimeria bovis *& Eimeria zuernii

A
  • Kingdom protista
  • Phylum apicomplexa
  • Order Eucoccidiida
  • DH: cattle (esp. calves)
  • Target organ: terminal ileum, colon
  • Very resistant oocyte
  • Diarrhea, anorexia, dull, dehydrated, dec. BW, intestinal lesions, fever, desentery, tenesmus, possible death
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Trypanosoma cruzi (Chagas disease/American trypanosomiasis) Dx

A
  • TESA/Wester blot
  • ELISA
  • IFA
  • IHA
  • Blood smear
  • Xenodiagnosis - let clean Reduviid bug feed on dog, check bug for development of infection
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Tick Paralysis

A
  • Ascending, flaccid paralysis
  • Neurotoxin in tick saliva
    • Disrupts nerve synapses in spinal cord
  • Reversible
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Nocturnal Periodicity

A

Mff present in blood during evening

(Dirofilaria immitis, heartworm)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Toxoplamsa gondii Clinical Signs (small ruminants)

A
  • Sheep
    • infective ovine abortion
    • inflammation of fetal cotyledons
  • Goats
    • abortions
    • fever
    • lethargy
    • diarrhea
    • dec. appetite
    • death
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

*Dirofilaria immitis *(heartworm) Pathogenicity

A
  • Vessel wall irritation
  • Produce pro-inflammatory molecules → induce inflammation of vessels
    • Villous endarteritis
  • Vascular resistance - inc. cardiac workload
  • Dec. cardiac output - dec. organ perfusion w/ blood
  • Coughing
  • Hemoptysis - blood in sputum
  • Exercise intolerance
  • Vena Cava syndrome
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

*Sarcocystis *spp. Clinical Signs

A
  • Sheep - usually acute
    • anemia, anorexia, fever, death
  • Goats
    • weakness, anorexia, fever, death
    • abortion
  • Pigs - mild infection
    • usually asymptomatic
    • poor growth, meath quality, & wt. gain reduced
    • abortion
  • Humans
    • anorexia, ab. pain, diarrhea
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Phylum Platyhelminthes

A

Flatworms

  • Digestive system is blind sac
  • Usually hermaphroditic
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Parasite Concerns During Pregnancy

A
  • Impact on puppies
    • Toxocara canis (round worms) - transplacental
  • Impact on maternal health
    • Toxocara canis
    • Ancylostoma caninum - transmammary risk of infection post-partum
    • Heartworm disease
    • Fleas
    • Ticks
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Complete Periodicity

A

Mff may dissappear from peripheral blood at some point in 24 hr period

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Tritrichomonas blagburni (Feline trichomoniasis) Dx

A
  • Direct fecal smear
  • Culture
  • PCR
  • Colonic biopsy
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Diurnal Periodicity

A

Mff present in blood during daytime

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Ectoparasite

A

Parasite lives outside of host. Infestation.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Definitive Host

A

Adult/Sexually mature parasite resides in/on this host

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Class 1 Heartworm Disease

A

Asymptomatic to mild

  • No rad signs
  • No abnormalities in CBC/chemistry
  • No anemia
  • Slight loss of condition
  • Fatigue on exercise
  • Cough (rare)
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Parasitiasis

A

Parasite present in host but no outward signs of disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Homo/monoxenous
Only 1 host (definitive).
18
Pediculosis
Lice infestation
19
Ovoviviparous
* Characteristic of nematodes * Release egg w/ single L1 larvae * Ex. *Strongyloides westeri*
19
*Toxoplasma gondii* Life Cycle
21
Class Trematoda
* Phylum platyhelminthes * "Flukes" * All parasitic * Endoparasites
22
Parasitism vs. Predation
Ideally, parasite does not want to kill host (aka it's food source)
22
*Heterobilharzia americana* "Canine Blood Fluke"
* Phylum platyhelminthes * Class trematoda * IH: aquatic snail * DH: Dog, bobcat, raccoon * Often asymptomatic * D/V, wt. loss, lethargy, hypercalcemia, skin irritation, inflammation * Target organs: mesenteric & hepatic veins * Dioecious * "Swimmer's itch" (cercarial dermatitis) in humans
23
Microfilariae (Mff)
Prelarval stage of *Dirofilaria immitis *(heartworm) found circulating in blood
24
Ag test: Positive Microfilariae test: Negative (*Dirofilaria immitis *aka heartworm Dx)
* Maturing infection, Mff not yet in circulation * Use of preventative or microfilaricide w/ out removing adult worms * Unisex/Occult (female) infection * Dog's immune system responds to Mff's * Failure to use Mff concentration test
24
*Giardia *spp.
* Phylum protista * Direct life cycle * DH: most mammals, birds, amphibians * Vegetative stage: * 2 nuclei * 8 flagella * Ventral groove * Looks like smiley face * In loose feces * Cyst stage * resistant * in normal feces * 4 nuclei
24
Siphonapterosis
Flea infestation
25
*Giardia* Clinical Signs
* Chronic - villus atrophy * Intermittent diarrhea * loose * mucoid, not watery * Majority are asymptomatic
26
Oviparous
* Characteristic of nematodes * Releases egg with single stage or morula * Ex. *Haemonchus contortus*
27
*Tritrichomonas blagburni* "Feline Trichomoniasis"
* Kingdom protista * DH: cats (esp. younger) * No known IH, transmission unknown * Large-bowel disease * chronic diarrhea * spontaneous resolution * relapse * tenesmus * flatulence * Anal irritation * Fecal incontinence
27
*Stomoxys calcitrans*
"Stable Fly" * Phylum arthropoda * Class insecta * Order diperta * Family muscidae * Hosts: domestic animals, humans * **Males and Females feed on blood** * Mechanical vector * Terrestrial larvae (decaying plant material) * Affected by insecticides
27
Heartworm Treatment Follow Up
* Treat Mff * macrocyclic lactone @ sufficient dosage * minimize potential for resistance development * monitor for anaphylaxis * minimize spread of disease within 1 mile radius * Retest for heartworm 6 mos. post-treatment
28
Incomplete Periodicity
Mff never dissappear completely from peripheral blood at some point in 24 hr period (Dirofilaria immitis, heartworm)
29
Superfamily Strongyloidea
* Phylum nematoda * Medium-sized * Large mouths * Prominent copulatory bursa (males) * Direct life cycle * often extensive larval migration * Non-larvated eggs in feces
29
*Cystoisospora *(*Isospora*) spp.
* Kingdom protista * Phylum apicomplexa * Order Eucoccidiida * Stenoxenous * Monoxenous (direct life cycle) * DH: dogs, cats, pigs, humans (esp. AIDS patients) * Sexual & asexual reproduction * Oocyst - 2 sporocysts w/ 4 sporozoites each
30
Heartworm Treatment Protocols
Class 1, 2: Fast kill * Admin deep IM injection twice, 24 hrs apart * Rest 1 mos. post-final treatment Class 3: Slow kill * Admin single dose immiticide, followed in 1 mos. by 2 injections of immiticide, 24 hrs apart * Rest 1 mos. post-final treatment Caval syndrome * sx removal of heartworm from RA (don't break them! can induce major allergic response!!) * slow kill treatment Pain management for deep IM injections
31
Class Arachnida
* Phylum arthropoda * 2 body regions * head * body * Simple metamorphosis
32
Caval Syndrome (Class 4 Heartworm Disease)
Severe * Unstable animal * Poor prognosis * Cardiac cachexia (wasting) * Constant fatigue * Persistent cough * Dyspnea * +/- signs of R sided heart failure * Acites * Jugular vein distention (JVD) * Anemia ( * Proteinuria (\>2+) * Rad signs * RV +/- RA enlargement * PA enlargement * Pulmonary thromboembolism (PTE) * Adult worms detected in echocardiogram
33
Visceral Larval Migrans (VLM)
* Zoonosis of canine ascarid in humans (paratenic host) * children that eat dirt * Chronic granulomatous lesions * often in lier, lungs, brain (NLM), eye (OLM) * Hepatomegaly * Wt. loss * Dec. appetite * Persistant cough
34
*Dirofilaria immitis *(heartworm) Life Cycle
Mff released by female worm → Mff enter circulation → Mosquito feeds on infected dog → Mff become L3 → Mosquito feeds on other dog, infects it with L3 → L3 molts during migration to heart & lungs → Adult worms mature in heart & lungs → Adult worms mate → produce Mff Prepatent period: 5.5-7 mos
35
Facultative Parasite
Capable of living either free or as a parasite
35
Phylum Sarcomastigophora
* Kingdom protista * Distinct nucleus * Move via flagella * Asexual repro. * Direct & indirect life cycles
36
*Taenia pisiformis* "Rabbit Tapeworm/Abdominal Bladderworm"
* Phylum platyhelminthes * Class cestoda * Order cyclophyllidea * IH: rabbit, hare * DH: dog * Infected by ingestion of raw/undercooked meat * Metacestode/larvae: cysticercus * Egg: Taeniid type * Hexacanth embryo (oncosphere)
37
*Ancylostoma caninum* "Hookworm"
* Phylum nematoda * Superfamily strongyloidea * DH: dog * Target organ: small intestine * 3 pairs of scleritized teeth * Non-embryonated egg shed in feces → embryonates → L1 develops → hatches → molts to L2 → matures to L3, remains ensheathed (no molt!) → infects DH * Black/tarry diarrhea, poor appetite, hemorrhagic pneumonitis * CLM in humans
39
Urban Cycles
Involves parasites of companion/food animals
40
*Trypanosoma cruzi* (Chagas disease/American trypanosomiasis) Life Cycle
Trypomastigotes passed in feces of IH → enter host via bite wound, scratch, mucus membrane → Trypomastigotes enter circulation → enter cells of spleen, liver, lymphatics, muscle → cell rupture, trypomastigotes remain in circulation OR reinfect → Epimastigotes → replication via binary fission
41
*Toxocara canis* "Canine Ascarid"
* Phylum nematoda * Superfamily ascaroidea * DH: dog * Target organ: small intestine * **Egg w/ L2 is infective stage** * Death is rare * Pneumonia, V/D, pot-belly, focal lesions in CNS → neurologic disorders * Zoonotic: VLM
41
*Sarcocystis* spp.
* Kingdom protista * Phylum apicomplexa * Order eucoccidiida * IH: herbivore (ingests sporocysts in DH feces) * Target organs: schizonts in endothelal cells of blood vessels of brain, liver kidney. Form sarcocysts in skeletal & cardiac muscle. * DH: carnivore * Target organ: intestines
42
Patent Period
time parasite can be detected in host
42
*Rhiphicephalus sanquineus* "Brown Dog Tick"
* 3 host tick * mainly dogs * Tick paralysis * Disease vector * Only tick species to infest homes
43
*Eimeria bovis*
* Kingdom protista * Phylum apicomplexa * Order Eucoccidiida * DH: cattle (esp. calves) * Target organ: terminal ileum, colon * Gametogony causes majority of damage * Oocysts present when disease begins * Prepatent period: 5-15 days * Mucosal damage
44
Canine Ascarid Infection - Colostral/Lactogenic Transmission
* Nematode * Hypobiotic L2 in mammary tissue → puppy ingests colostrum → L2 go directly to stomach → small intestine * No migration
45
Parasitic Gastroenteritis (PGE)
* Caused by Trichostrongyloid nematodes * Esp. *Ostertagia ostertagi* * Diarrhea (cattle \> sheep, goats) * Anemia (cattle \< sheep, goats * Bottle jaw * Weanlings at grass are most susceptible * Adults fairly immune * Total eradication unlikely, must move to worm-free pasture
46
Hookworm Infection - Skin Penetration
* Strongyloid nematode, L3 * Dogs * Blood/lymphatic vessels → heart → lungs → alveoli → bronchi → swallowed → GI * mature in small intestine * Dogs \>3 mos. * Blood/lymphatic vessels → encyst in muscle (hypobiotic) * Populate mammary glands * Repopulate small intestine
47
Hookworm Infection - Paratenic host
* Strongyloid nematode, L3 * Ingests rodent host * Hypobiotic L3 (no development in rodent) * Adult matures in small intestine * No migration
48
Vena Cava Syndrome
Too many adult heartworms in R atrium and vena cava. Obstruct blood flow & interfere w/ tricuspid valve closure.
48
Ticks
* Phylum arthropoda * Class arachnida * Order acarina * All stages feed on blood * Tick paralysis * Transstadial, transovarial disease transmission * Body division * Head - capitulum * Body - idiosoma (fused cephalothorax & abdomen)
49
Parasitism
Relationship where one species, the parasite, lives on or in another species, the host, and may cause harm. Parasite is metabolically dependent on host.
50
*Dirofilaria immitis* (heartworm) Tx
* Melarsomine dihydrochloride (Immiticide) * Kills adult worms (nothing younger than L4) * Monthly use of preventatives - "slow kill" * Alternative tx, takes mos. to years to work * Surgical removal of worms
51
*Sarcoptes scabiei* "Scabies"
* Phylum arthropoda * Class arachnida * Order acarina * DH: cattle, goats, pigs (most mammals) * Target organ: tunnel through dermid * Eggs/feces can induce hypersensitivity rxn * Long, non-jointed pedicles * Females: suckers on pairs 1, 2 * Males: suckers on pairs 1, 2, 4
52
Canine Ascarid Infection - Prenatal/Transuterine Transmission
* Nematode * Hypobiotic L2 mobilize in late pregnancy → liver of fetus → molt to L3 → in lungs @ birth → alveoli → bronchioles → trachea → coughed up → swallowed → stomach → L4, L5 in small intestines → adult
53
Indirect Life Cycle
1 or more intermediate hosts
53
Flea Control
* Host-targeted insecticides * Kill larvae & adults * Environmental insecticides * Mechanical methods * Vacuum, wash pet bedding * Remove vegetation, litter * Prevent wildlife interaction
54
Heartworm Treatment
* Prevent L3, L4 from maturing * Macrocyclic lactone * Avoid avermectin at first * Kill all Mff at once → major allergic response * Weaken the adult heartworm * Tetracycline * more susceptable to immiticide * Prevent emboli formation * rest until 1 most post-immiticide treatment * Optimize efficacy of immiticide * Allow L5 to become mature adults ## Footnote **IMMITICIDE TOXIC TO CATS**
55
*Giardia *Life Cycle
Ingestion of cyst → excystation in duodenum → replication via binary fission → encystment in lower intestinal tract → infective cysts passed in feces Prepatent period: 1 wk
56
*Cytauxzoon felis* Pathogenicity/Clinical Signs
* Fatal form: * fever (up to 105ºF) * depression, lethargy * anorexia * jaundice * death \< 1 wk * Non-fatal form: * may be asymptomatic * similar signs as fatal form * persistent parasitemia * Schizogenous phase most destructive * Infected macrophages can block blood vessels
57
Schistosomes
* Trematode * Tubular body * Dioecious * Female fits into central groove of male * Mature in blood of DH
58
Hookworm Infection - Oral
* Strongyloid nematode, L3 * Rare transmission, not usually found in nature * No larval migration * Adults mature in small intestine * Prepatent period: 15-18 days
59
Euryxenous
Wide host range
60
Occult Infection (heartworm)
* Hidden infection * Single sex or low number of *Dirofilaria immitis* * Mff not detectable in peripheral blood
60
*Toxoplasma gondii* Clinical Signs (cats)
* Studies indicate cats shed once (sometimes relapses occur, but rare) * Fever * Anorexia * Jaundice * Ab. pain/discomfort * Hepatitis * Pancreatitis * Neurological problemts * Respiratory interference * Death
61
Cysticercus
* Class cestoda (tapeworms) * Order cyclophyllidea * Larva/metacestode * Single, invaginated scolex * Vertebrate IH
62
Class Arachnida, Order Acarina
Ticks & Mites * Phylum arthropoda * Simple metamorphosis
64
Subclass Digenea Life Cycle
* IH (snail): miracidium → Sporocyst → Redia → Daughter rediae/Cercariae * Environment: Cercaria → Metacercaria (infective stage)
65
Complex (Complete) Metamorphosis
Phylum arthropoda Class insecta
66
Order Mallophaga
Chewing/Biting Lice * Phylum arthropoda * Class insecta * **Head wider than thorax** * Simple metamorphosis * Feed on dead skin * Stenoxenous * Spp. affecting birds, mammals
67
*Tritrichomonas foetus* Clinical Signs
Bulls (chronic carriers) - often asymptomatic Cows * vaginitis * cervicitis * endometritis * pyometra * mucopurulent discharge * early or late term abortion
68
*Cystoisospora suis*
* Kingdom protista * Phylum apicomplexa * Order Eucoccidiida * Direct life cycle * DH: pigs (esp. suckling piglets) * Infected via contaminated feces * Pasty/liquid diarrhea (no blood in feces) * Villous atrophy, necrotic enteritis, crypt hyperplasia, secondary infection
70
Stenoxenous
Narrow host range
72
Superfamily Trichuroidea
* Phylum nematoda * Short, fat posterior ends * Long, whip-like anterior end * Beaded esophagus (stichocyte) * Usually direct life cycle
73
Chronic American Trypanosomiasis
* *Trypanosoma cruzi* in dogs * No circulating trypomastigotes (harder to dx) * Dilated, enlarged heart * Arrhythmia * Lethargy * Respiratory difficulties * Anemia
74
Frass
* Blood excreted by adult fleas * Reddish-black pellets in the haircoat * AKA "flea dirt" * Eaten by flea larvae
74
Heartworm Parasite Control Treatment Recommendations
* Year-round broad-spectrum parasite control for heartworm, intestinal parasites, ticks * Anthelmentic tx every 2 wks until 2 mos. old, then monthly * Maintain pregnant/nursing dams on broad-spectrum control products
76
Rediae/Redia
* Trematode (digenea) * In snail (IH) * Stage doesn't always occur * Produces daughter rediae or cercariae
77
Family Argasidae
Soft-bodied Ticks * Phylum arachnida * Order acarina * Can't see head from dorsal view
78
*Eimeria zuernii*
* Kingdom protista * Phylum apicomplexa * Order Eucoccidiida * DH: cattle (esp. calves) * Target organ: terminal ileum, colon * Schizogony causes majority of damage * No oocysts present yet (animal typically doesn't live long enough for gametogenesis to occur) * Bloody diarrhea (can "shoot out" when calf coughs) * Mucosal damage
79
*Sarcocystis* spp. Life Cycle
80
Order Diptera
* Phylum arthopoda * Class insecta * "True"/Two-winged flies (includes mosquitoes) * Ectoparasites * Permanant * Temporary * Perform **myiasis** * Complex metamorphosis
82
*Diplylidium caninum* "Cucmber Seed/Double-pored Tapeworm"
* Phylum platyhelminthes * Class cestoda * Order cyclophyllidea * IH: flea, dog louse * DH: Dogs, cats, humans (esp. children) * Target organ: small intestine * Metacestode/larvae: cysticercoid * Armed scolex w/ 4 circular suckers * Egg packets * Prepatent stage: 2-3 wks * Usually not pathogenic, may itch due to migrating proglottids * Heavy infections: constipation, diarrhea, pot-belly
84
Hookworm Infection - Lactogenic
* Strongyloid nematode, L3 * L3's in milk * Hypobiotic larvae migrate to mammary gland * Adult matures in small intestine * No migration
85
Periparturient Rise (*Haemonchus contortus*)
* Massive outpouring of worm eggs (nematode trichostrongyle *H. contortus*) by ewes/does while lactating * Prolactin may suppress immunity to worms * Sources * Hypobiotic larvae resuming development * Ingestion of larvae that have overwintered on pasture * Increased egg output from existing worm population * Worm load maintained rather than expelled → massive pasture contamination of L3
86
*Toxoplasma gondii* Dx
* Fecal float * ELISA, IFA * 1 pos. serum sample indicates past infetion * 16x rise in titer in serum taken 2-4wks after 1st sample indicates acute, acquired infection * Biopsy * PCR
87
Acute American Trypanosomiasis
* *Trypanosoma cruzi *in dogs * Trypomastigotes circulating * Myocarditis * Pale MM * lethargy * Hepatomegaly * Splenomegaly
89
Hookworm Infection - Prenatal/Transplacental
* Strongyloid nematode, L3 * Rare * L3 in blood stream → placenta → feus * Hypobiotic larvae * Occurs during pregnancy
91
Evolution of Parasitism
1. Parasite co-evolved w/ host - began as symbiotic relationship? 2. Parasite has recently colonized the host * more likely to be pathogenic * result of keeping animals in environment to which they are not well adapted
92
*Cryptosporidium *spp. Oocyst Erradication
* Heat to 64-72.4°C * Freeze to -20°C * UV irradiation * Chlorine * Hypochlorite * Hydrogen Peroxide * Iodine (not very well) * Does NOT work: * ethanol * isopropanol * Lysol * Pine-sol * formaldehyde * betadine
92
Mites
* Phylum arthropoda * Class arachnida * Order acarina * Larva - 6 legs * Adults - 8 legs * Pedicels - legs/stalks (used to ID) * Body divisions * Head - gnathsoma * Body - idiosoma
94
Phylum Nematoda
* "Roundworms" * Dioecious * Females \> males * Complete digestive system * Cervical alae * Non-segmented * Copulatory bursa (males) - hold onto females * Spicules - equivalent to penis * Mouth possibilities * Sclertotized buccal capsule * Lips * Teeth * Dentigerous ridges
94
*Cytauxzoon felis *Life Cycle
Tick ingests piroplamsm in RBCs of infected host → Piroplasms travel to salivary glands of tick → asexual reproduction → tick feeds on DH → prioplasms develop into schizonts (in macrophages) → merozoites released upon macrophage rupture → enter RBCs → develop into piroplams Transstadial transmission: tick can pick up protozoa in its larval or nymphoid stage and continue carrying the protozoa through its molts
95
Family Ixodidae
Hard Ticks * Head visible * Scutum * Covers all dorsal part of male * Covern cranio-dorsal part of females * Ornate or inornate * Hypotome - mouthpart * Anal groove * Festoons - wavy ridges on caudal edge
96
Superfamily Dracunculoidea
* Phylum nematoda * Female in SubQ * Indirect life cycle * Ex. Guinea worm
96
*Ostertagia ostertagi* "Brown Stomach Worm/Bankrupt Worm"
* Phylum nemotoda * Superfamily trichostrongyloidea (H**_O_**TC) * DH: ruminants * Target organ: adults in lumen, larvae in gastric glands * Modest anemia * Protein losing gastropathy * Larvae destroy gastric glands during development * Protein leaked into abomasum, pepsinogen leaked into serum * HCl-producing cells damaged * Abomasal pH goes neutral * Pepsinogen not converte to pepsin (decreased protein digestion) * Loss of acid bacteriostasis (bacterial overgrowth)
97
*Ixodes scapularis* "Black Legged Tick"
* AKA Deer tick * 3 host tick * birds * small mammals * cattle, sheep, horses * Disease vector * Lyme * Super tiny
99
Obligatory Parasite
Must live as parasite to survive.
100
*Spirometra mansonoides*
* Phylum platyhelminthes * Class cestoda (tapeworms) * Order pseudophyllidea * 1st IH: copepod * 2nd IH: frog, rat, snake * Ingests 1st IH * encyst in muscle * DH: dogs, cats * Target organ: small intestine * Paratenic host: humans * Ingest copepod/muscle of 2nd IH: encysts in muscle tissue, periorbital area * Coiled, gravid uterus * Eggs: operculated, contain coracidium (emerges upon contact w/ water) * Prepatent period: 7-14 days * Most cases asymptomatic * V/D, wt loss may occur
101
Cutaneous Larval Migrans (CLM)
* Zoonosis of *Ancylostoma* spp. (strongyloid nematode) * "Sand worms", "plumber's itch", "creeping eruption" * L3 penetrates skin and migrates looking for target * Leaves winding, red pruitic lesions * Rarely enter small intestine to mature
102
*Trichinella spiralis* "Trichina Worm"
* Phylum nematoda * DH & IH: pigs, rats, bears, wild boar, humans, horses, foxes, other mammals * Target organ: adults in small intestine; larvae in striated muscle * Larviparous * Dx: muscle biopsy, skin sensitivity (humans), ELISA, slaughterhouse inspection
104
*Tritrichomonas foetus* Dx
* Recover organisms * Females: vaginal, uterine flush * Males: prepucial wash * PCR * Direct smear * Culture
105
Facultative Myiasis
* Done by some species in order diptera (true-flies) * Normally: eggs deposited in garbage, feces, rotten carrion * **Occasionally: eggs deposited in contaminated wounds** * Early lesions * Dermatitis * Presence of maggots * Pungent odor * Inflammation (have been used medically to clean out necrotic tissue in wounds)
107
Paratenic/Transport Host
Immature stage consumed. Parasite does not undergo further development. Remains encysted until host is eaten by definitive host.
108
Superfamily Acanthocephalans
* Phylum nematoda * Eversible proboscis * Indirect life cycle * IH: arthropod * Adults in small intestine of vertebrates * Fluid filled body cavity
109
Superfamily Filarioidea
* Phylum nematoda * Long, slender worms * Male has corkscrew tail * Indirect life cycle * IH: arthropod * Larviparous
110
Hyatid Cyst
* Class cestoda (tapeworms) * Order cyclophyllidea * Larva/metacestode * Brood capsules w/ 100s-1000s protoscolices * Unilocular or Multilocular * Multilocular can "bubble-out" and spread like cancer * Zoonotic - humans can be IH
111
Order Siphonaptera
Fleas * Phylum arthropoda * Class insecta * Complex metamorphosis * Only adult is on host * Lays egg on host → egg falls off → larvae & pupae develop off the host * 95% infest mammals * Mate once * Larvae eat frass * Found in crevices, rugs * 2 molts, 3 instars * Vectors (plague)
113
*Tritrichomonas foetus* "Bovine Genetal Trichomonosis"
* Phylum protista * Venereal disease of cattle * 3 anterior flagella * 1 posterior flagella * Undulating membrane * Axostyle (axial rod) * Pseudocyst * Binary fission (asexual) * Causes early embryonic death, abortion
115
Characteristics of Kingdom Protista | (Protozoans)
* Free living or parasitic * Eukaryotic * Unicellular * nucleus * mitochondria * ER * Golgi app. * Flagella * Cilia * Pseudopods * Undulating ridges, subpellicular microtubules * Asexual & sexual reproduction * Auto or heterotropic
117
Sporocyst
* Trematode (digenea) * Develops in snail (IH) * Produces rediae or cercariae
117
Heartworm Case Differential Diagnoses
* Intestinal parasites - ancylostoma (hookworm) * Collapsing trachea * Allergic bronchitis * Dog * kennel cough * Cat * feline asthma
118
*Trypanosoma cruzi* "Chagas Disease/American Trypanosomiasis"
* Kingdom protista * DH: dogs (esp. sporting & working), cats, opossums, armadillos, racoons, rats, humans * Vector/IH: Reduviid bug * Target organs * Trypomastigote - circulating blood * Amastigote - smooth/cardiac muscle, neural cells
119
Mosquito - Male vs. Female
* Female * Feeds on blood * Pilose antennae * Long (anopholene) or short (culicine )palps * Male * Pollinator * Plumose antennae (looks like feather) * Clubbed (anopholene) or long (culicine) palps
120
Cysticercoid
* Class cestoda (tapeworms) * Order cyclophyllidea * Larva/metacestode * Single scolex * Not invaginated * Invertebrate IH
122
How do parasites escape immunity?
* Molecular mimicry * Antigenic variation * Secretion of substances that disable local immune response
123
Class 2 Heartworm Disease
Mild to moderate * Mild rad signs * RV enlargement * PA enlargement * Mix alveolar/interstitial pattern * Anemia (\>20% pcv) * Mild proteinuria * +/- live, kidney changes * May be clinically free of clinical signs or show mild signs
124
Hypobiosis
Arrested development/dormancy of nematode larvae (usually L4) * More resistant to anthelmentics * Extends prepatent period
125
Class Insecta
* Phylum arthropoda * Segmented body * Head * Thorax * Abdomen
127
*Toxoplasma gondii*
* Kingdom protista * Phylum apicomplexa * Order eucoccidiida * IH: most mammals * Encyst in tissues (liver, lungs, spleen, lymph nodes, brain, heart & skeletal muscle, eye) * DH: felines * Target organ: intestine * Transmission * Acquired: oocysts, raw/undercooked meat * Congenital: transplacental
128
Class Cestoda
* Phylum platyhelminthes * Tapeworms * All parasitic * Hermaphroditic * Body of scolex & proglottids * No digestive, circulatory, respiratory, skeletal system
129
Canine Ascarid Infection - Paratenic Host Transmission
* Nematode * Dog ingests paratenic host w/ encysted L2 → L2 goes directly to stomach → matures in small intestine * No migration
130
*Fasciola Hepatica* "Liver Fluke"
* Phylum platyhelminthes * Class trematoda * Subclass digenea * IH: aquatic snail * DH: cattle, sheep, goats * Target organ: bile ducts * Most pathogenic fluke * Acute/Subacute/Chronic Fascioliasis
130
*Giardia *Dx
* Fecal float * Direct smear of diarrhetic feces * ELISA
132
*Sarcocystis *spp. Dx
* Fecal float * Biopsy/necropsy * IH: schizont rosettes, sarcocysts * Western blot * Ab in serum or CSF (*S. neurona*) * PCR * History of presence of dogs/cats on premises
133
Larviparous
* Characteristic of nematodes * Eggs retained in uterus * "Gives birth" to live larvae or microfilariae * Ex. *Dirofilaria immitis*
134
Parasitosis
Parasite present in host and causing clinical signs of disease.
135
136
Order Cyclophyllidea
* Phylum platyhelminthes * Class cestoda * "True tapeworms" * Scolex (head) * Armed or unarmed * 1-2 sets of reproductive organs * Lateral genital pore * NO uterine pore * Shed gravid proglottid to release egg * Larvae - metacestode * cysticercoid * cysticercus * coenurus * Hyatid cyst
137
Order Pseudophyllidea
* Phylum platyhelminthes * Class cestoda * "Pseudotapeworms" * Scolex: bothria * 1 set reproductive organs * Genital pore * **Uterine pore: shoot out eggs from proglottid** * Operculated eggs: coracidum * Larvae: metacestode * Procercoid - in 1st IH (copepod) * Plerocercoid/Sparganum - in 2nd IH (vertebrate, frog)
138
*Amblyomma americanum* "Lone Star Tick"
* Three host tick * Rodents, rabbits * Deer, cattle, horses, sheep * Tick paralysis * Disease vector * RMSF * Anaplasma
139
Prepatent Period
time from infection until diagnostic stages
139
*Cryptosporidium* spp. Life Cycle
140
Superfamily Oxyuroidea
* Phylum nematoda * "Pinworms" * Double-bulbed esophagus * Direct life cycle, no migration * Eggs often left on perineal area * Scotch tape test
142
*Cystoisospora *& *Eimeria* spp. Life Cycle
Sporulated oocyst ingested by DH → Sporozoites excyst → Trophozoite undergoes schizogony (asexual repro) in intestinal epithelium → Type I schizont infects another epithelial cell → Type II schizont → gametogony (merozoites → gametocytes) → microgametocyte (male) and macrogametocyte (female) join → zygote → detatch from epithelium → oocyst → release in feces → sporogony
143
Cercariae/Cercaria
* Trematode (digena) * Leaves snail (IH) * Has head & tail (resembles sperm) * Will do 1 of 3 things * Infect/penetrate 2nd IH * Encyst on vegetation * Infect/penetrate DH
144
*Platynosomum concinnum (fastosum)* "Lizard Poisoning Fluke"
* Phylum platyhelminthes * Class trematoda * 1st IH: Terrestrial snail * 2nd IH: Anole lizards * Paratenic host: pill bugs * DH: cats * Normally no severe disease * Lizard poisoning: wt. loss, emaciation, D/V, jaundice, death * chronic: englarge bile ducts & gall bladder, liver failure * Target organs: liver, bile duct, pancreatic duct
146
Superfamily Trichostrongyloidea
* Phylum nematoda * Hair-like worms * Tiny mouths * Copulatory bursa (males) * Direct lifestyle * Non-larvated eggs * "HOTC" nematodes - eggs all look the same * DH: ruminants * Target organ: abomasum, small intestine
147
Obligatory Myiasis
* Done by species in order diptera (true flies) * Larvae **must use host** to complete life cycle * Adult deposits egg in * Living tissue * **Uncontaminated wounds** * Soft tissue * Will NOT find in feces, garbage * Ex. *Cochliomyia hominivorax* (Definitely do NOT want to use medically. They eat the good stuff.)
148
Canine Ascarid Infection - Direct Transmission
* Nematode * Dogs * Ingest egg w/ L2 → larva hatches in duodenum → penetrates intestine → mesenteric lymph nodes → liver → heart → pulmonary arteries → lungs → molts to L3 → alveoli → bronchioles → trachea → coughed up → swallowed → stomach → molts to L4, L5 in small intestine → adult * Prepatent period 3-4 wks * Dog \>3 mos. * Ingest egg w/ L2 → larva hatches in duodenum → penetrates intestine → enters circulation → L2 encysts (hypobiotic) in various tissues, no maturation
149
Diagnostics for Possible Heartworm
* Fecal float * Heartworm Ag test * Mff test * +/- chest rads * +/- CBC, Chemistry
150
Type I Ostertagiasis
* Caused by trichostrongyloid nematode *Ostertagia ostertagi* * Large number of larvae acquired from pasture * Moroccan leather appearance of abomaum mucosa * Substantial damage of gastric glands during migration (larvae all leave at once) * High egg counts * Rapid onset of clinical signs * Most susceptible in herd are affected * Good prognosis w/ tx * move to clean pasture
151
Heartworm Screening
* PE every 6-12 mos. * Annual hearthworm test (dogs) * test cats only if clinical signs are present, before placing on prevention * Fecal exam * Puppies \> 1 yr: every 3-6 mos. * Adults: annually * In endemic regions: annual testing for vector-transmitted pathogens
152
Endoparasite
Parasite within host. Infection.
154
*Oxyuris equi* "Large Equine Pinworm"
* Phylum nematoda * Superfamily oxyuridae * DH: horse * Target organ: large intestine * Eggs: asymmetrical, operculated, unembryonated when laid * Female deposits egg in perianal region within sticky matrix, where the egg embryonates * Eggs fall into bedding food and the horse ingests them * Pruritis ani/"Seat itch" * risk of secondary bacterial infection * Fecal float not dependable * Scotch-tape test
155
Type II Ostertagiasis
* Caused by trichostrongyloid nematode *Ostertagia ostertagi* * Uncommon * Ingestion of L3 → hypobiosis in gastric glands * No clinical disease initially * Conditions improve → mass eruption of larvae (can be \>100,000) * Usually yearlings, heifers, older animals * Diagnostic clinical signs: rapid onset of severe PGE in only a few animals * Variable egg counts * High serum pepsinogen (manily looked at in research) * Mucosal reddening, edema
157
Sylvatic Cycles
Involves parasites of wild animals infecting humans
158
*Chrysops* spp.
Deer fly * Phylum arthropoda * Class insecta * Order diptera * Family tabinidae * Females feed on blood * Males feed on honeydew, nectar * IH/vector * Aquatic larvae * Herbivorous larvae * Insecticides NOT useful
159
Superfamily Ascaroidea
* Phylum nematoda * Very large * 3 anterior lips * Direct life cycle * Non-larvated eggs in feces * thick-shelled, morula
161
Ag test: variable Microfilariae test: Negative or Positive (*Dirofilaria immitis* aka heartworm Dx)
Fluctuating Ag level due to # of female worms, age of worms, quality of sample
163
*Cytauxzoon felis* Dx
* Blood smear * PCR * Biopsy/necropsy of visceral organs * histiocytes - nucleus displace, cytoplasm filled w/ granules
165
*Anoplocephala perfoliata* "Lappeted Tapeworm"
* Phylum platyhelminthes * Class cestoda * Order cyclophyllidea * IH: mite * DH: horse * Target organs: intestines, congregates near ileocecal valve * Adults: short & wide w/ lappets ("bowties") behind each sucker * Metacestode/larvae: cysticercoid * Egg: hexacanth embryo, pyriform apparatus * Predisposes to intussusception, impaction colic * Chronic symptoms: ill thrift, recurrent diarrhea, wt. loss * Indirect & serologic ELISA available
167
*Dirofilaria immitis* "Heartworm"
* Phylum nematoda * DH: dogs, cats, ferrets, sea lions, seals * Target organ: R ventricle, pulmonary arteries * IH/Vector: mosquito * Male is smaller w/ spiraled posterior end * Larviparous * Nocturnal and imcomplete periodicity
168
*Otodectes cynotis* "Ear Mite"
* Phylum arthropoda * Class arachnida * Order acarina * DH: dogs, cats, foxes, raccoons, ferrets (NOT zoonotic) * Target organ: external ear canal * thick, tarry exudate * Transmission: direct contact * Short, nonjointed pedicels * Females: suckers on leg pairs 1, 2, 4 * Males: suckers on all leg pairs * Permanent parasite * Dx: ear swab & smear
169
*Haemonchus contortus* "Barberpole Worm"
* Phylum nematoda * Superfamily trichostrongyloidea (**_H_**OTC) * DH: ruminants, esp. goats, sheep * Sheep/goats acquire little/no immunity * Prolactin suppresses immunity to worms even more * Periparturient rise * Target organ: abomasum * Lancet tooth at anterior end (for blood sucking) * Major cause of PGE * Anemia * Very fecund (high fertility) * Resistant to most anthelmentics
170
*Tabanus *spp.
Horse Fly * Phylum arthropoda * Class insecta * Order diptera * Family tabanidae * Females feed on blood * Males feed on honeydew, nectar * IH/vector * Aquatic larvae * Carnivorous larvae * Insecticides NOT usefull
171
Family Culicidae
Mosquitoes * Phylum arthropoda * Class insecta * Order diptera * Subfamilies * Anopholene * *Anopholes* spp. * Culicine * *Aedes* spp. * *Culex* spp. * Larvae are always aquatic * Pupae do not feed
172
Coenurus
* Class cestoda (tapeworms) * Order cyclophyllidea * Larva/metacestode * Many invaginated scolices (scolex) * Vertebrate IH
173
Simple (Incomplete) Metamophosis
Phylum arthropoda Class arachnida
174
*Sarcocystis cruizi* Clinical Signs
Dalmeny disease in Cattle * intermittent fever * Loss of condition * diarrhea * vaginitis * abortion (last trimester) * mild nervousness * dec. milk yield * anemia * death
175
Miracidium
* Trematodes (digenea) * Ciliated, penetrates snail
176
*Demodex canis* "Follicle Mite"
* Phylum arthropoda * Class arachnida * Order acarina * DH: dogs (NOT zoonotic) * Target organ: hair follicles & adjacent sebaceous glands * Permanent parasite * Localized or general demodectid acariasis * Alopecia * Pyoderma * 2° bacterial infections * Transmission: direct contact * Dx: * Deep skin scrape * Look at pustule/abcess contents * May see in fecal float (dog bits at itch, injests mite)
177
Pseudoparasite
An object mistaken for a parasite in diagnostics (ex. pollen in fecal float)
178
Equine Protozoal Myeloencephalitis (EPM)
* Horse is aberrant/dead-end host of *Sarcocystis neurona* * ​Normal DH: opossum * Normal IH: otter, raccoon, seal, skunk, armadilla * CNS disease * Schizonts destroy neuronal cells while developing * Merozoite release lyses cells * No sarcocysts develop * Lesions in spinal cord, brain, meninges * Clinical signs: head tilt, drooping eye/eyelid, facial paralysis, masseter atrophy, circling, stumbling, falling
179
*Cryptosporidium *spp.
* Kingdom protista * Phylum apicomplexa * Order eucoccidiida * Immunity to 1 species does NOT give immunity to others * DH: fish, birds, reptiles, mammals (including humans) * Target organ: intestine (esp. microvillus border) * Small oocysts * Risk of waterborne outbreaks * LOTS of diarrhea!! * yellow, profuse watery * usually self-limiting
180
Intermediate Host
Harbors larval/asexual/immature stages of parasite
181
Subclass Digenea
* Phylum platyhelminthes * Class trematoda * Leaf-like body shape * Schistosomes * Indirect lifestyle * IH usually a snail * Tandem or parallel male sex organs * Usually suck blood from host
182
Metacercariae/Metacercaria
* Trematode (digenea) * Encysts in environment or host * Infective stage (DH)
183
Heteroxenous
\>1 host (intermediate & definitive hosts.
184
*Eimeria *spp.
* Kingdom protista * Phylum apicomplexa * Order Eucoccidiida * Stenoxenous * Monoxenous (direct life cycle) * DH: birds, cattle, small ruminants, horses, rabbits, rodents, pigs * Sexual & asexual reproduction * Oocyst - 4 sporocysts w/ 2 sporozoites each
185
Direct Life Cycle
No intermediate hosts