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Semen analysis > viva > Flashcards

viva Flashcards

1
Q

What is aggregation?

A

Adherence of immotile spermatozoa to each other or motile spermatozoa to mucous or non sperm cells or debris

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2
Q

What is agglutination?

A

Motile spermatozoa sticking to each other

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3
Q

How do you grade agglutination?

A

Grade 1: Isolated
Grade 2: Moderate 10-50 sperms
Grade 3: Large > 50 sperms
Grade 4: Gross

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4
Q

leukocyte detection in semen is by:

A

Peroxidase activity

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5
Q

Time of evaluation of sperm motility: and why?

A

at 30 min: liquefaction time

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6
Q

What are categories of sperm movement?

A

1 - Progressive motility
2 - Non-progressive motility
3 - Immotile

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7
Q

What is purpose of eye piece reticle in semen analysis?

A

to count spermatozoa count

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8
Q

How do you assess sperm vitality?

A
  1. Dye exclusion

2. Hypo-osmotic swelling test

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9
Q

What is the principle of dye exclusion technique?

A

Damaged palms membranes of dead cells, allow the entry of membrane imermeant stains

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10
Q

What is the most commonly used dyes for vitality test?

A

Eosin-Nigrosin stain

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11
Q

How do you score vitality in Eosin-Nigrosin stain?

A

Live - white, Dead - red
faint pink - assessed alive
Leaky neck membrane

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12
Q

Incubation time for hypo-osmotic swelling test for IVF:

A

5 minutes

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13
Q

How do you score vitality in Hypo-Osmotic swelling test?

A

Swollen - alive

unswollen - dead

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14
Q

What normal sperm concentration?

A

number of spermatozoa in unit volume

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15
Q

What is total sperm number?

A

number x semen volume

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16
Q

What is the fixative for diluting semen?

A

Semen diluting fluid- sodium bicarbonate and 10mal on

17
Q

What is cryptozoospermia?

A

Absence of spermatozoa in either replicates

18
Q

What is ‘CASA’?

A

Computer Assisted Semen Analysis

Semen analysis (1 decks)

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