Virology Flashcards
What reasons would you have to order lab tests?
O diagnosis
O screening
O Monitoring
O research
What are some pre-analytical errors that can occur?
O mislabelled sample
O incorrect test tube used
O pt ID incorrect
O form not completed properly
O test request lost
O incorrect test ordered
O sample collection error
O transport error
O incorrect sample handling
What tests are used in the virology lab?
Oserology- antibody testing
O molecular - (pcr, Elisa)
What viruses are common in the prenatal period? (LIST 6)
O CMV
O parvovirus b19
O rubella
O hsv
O HIV
O EBV
O hepatitis b+c
o varicella zoster
O zika virus
What viruses are common in the perinatal/postnatal period? (List 6)
O HIV
Ohsv
O CMV
Oenterovirus
O Varicella zoster
O hepatitis b+c
o adenovirus
What viruses are common in the postnatal period? (List 5)
O enterovirus
O rotavirus
O CMV
O varicella zoster
O hepatitis
O influenza
O adenovirus
How do you determine what sample to take to isolate the virus?
Falco-oral transmission = stool
Resp droplets = vesicle fluid
Vertical = blood
Specimen + test for HSV
O vesicle fluid
O CSF
= HSV PCR
Enterovirus specimen and test?
O throat swab / stool
= Enterovirus PCR
Varicella zoster specimen + test?
O vesicle fluid /blood / clinically
=Varicella zoster PCR
= blood = Igm
What type of rash is in picture 2?
Maculapapular
What type of rash is in picture 3?
Vesicular
What type of rash is in picture 4?
Pustules
What type of rash is in picture 5?
Bulla
What type of rash is in picture 6?
Nodules
Measles presentation, specimen, + test
O presentation
> koplik spots
> maculopapular rash
> 3 C’s = conjunctivitis, cough, coryza
O specimen = blood, throat swab within 5 days of rash
O serology = IgM/G
Swab = PCR
Complications of measles
O Otitis media
O diarrhoea
O pneumonia
O encephalitis
Measles treatment +prevention
O vaccine 6 + 12 months
O treatment = supportive (vit A)
Rubella sx, specimen + test
O sx
> maculopopular rash
> many asymptomatic
O specimen
>blood = IgG/M (older)
> throat swab = PCR (congenital)
Complications of congenital rubella
O miscarriage
O congenital rubella syndrome ( healing loss, heart defects, glaucoma cataracts )
O abnormal fetal growth
HHV 6/7 sx, specimen, test
① fever without rash
② rash without fever
Specimen = clinical/blood (IgG/M or PCR)
Parvovirus sx, specimen, test
Sx
O slapped cheeks malar rash
O trunk maculopapular rash
Specimen = blood (IgG/M or PCR)
Mumps sx, specimen, test
Swelling of the parotid gland (parotomegaly)
Specimen = blood/ buccal swab (IgG/M or PCR)
CNS viruses, specimen + test
Enterovirus, Mumps, HSV 1, rabies -“ panel” done which screens for all ( CNS viral)
All CSF = PCR
Except rabies ( saliva = PCR )
