Video 1 - Ma'am Kath tutorial

Question 1

What are the different bacterial growth phase?

Answer 1

Lag Phase
Log Phase
Stationary phase
Death Phase

Question 2

no increase in number of living bacterial cells; metabolic activity happens

Answer 2

lag phase

Question 3

exponential increase in number of living bacterial cells

Answer 3

log phase

Question 4

plateau in number of living bacterial cells; rate of cell division and death are equal

Answer 4

stationary phase

Question 5

exponential decrease in number of living bacterial cells

Answer 5

death or decline phase

Question 6

toxin accumulation/depletion of nutrients

Answer 6

log phase

Question 7

cleaning or washing of glasswares

Answer 7

sterilization and decontamination

Question 8

setup of pressure cooker for Sterilization

Answer 8

15 psi for 15 mins

Question 9

setup of pressure cooker for decontamination

Answer 9

15 psi for 30 mins

Question 10

reasons for errors

Answer 10

air control: chattering, air pollution
hot agar killed mesophiles

Question 11

What is the plating used for food samples? Betamax and Isaw

Answer 11

Spread plating

Question 12

What is the plating used for milk samples?

Answer 12

yoghurt

Question 13

agar used for food samples (betamax and isaw)

Answer 13

PCA

Question 14

agar used for milk samples
(yoghurt)

Answer 14

MRSA

Question 15

What is the diluent used for food samples? (betamax and isaw)

Answer 15

peptone water

Question 16

diluent used for milk samples

Answer 16

MRS

Question 17

What are the different groups of microbes based on temperature?

Answer 17

psychrophiles
mesophiles
thermophiles
hyperthermophiles

Question 18

grow above 50 deg celsius

Answer 18

thermophiles

Question 19

peak temp of mesophiles

Answer 19

37.5 deg celsius

Question 20

5-10 deg celsius or below

Answer 20

psychrophiles

Question 21

extremely hot temperatures

Answer 21

hyperthermophiles

Question 22

groups of bacteria usually grown in lab

Answer 22

mesophiles