Vibrio Cholerae-Virulence Factors Involved in Colonization Flashcards
1
Q
Who is the culprit?
A
single polar flagellum
2
Q
Who are the culprit weaklings?
A
- NONmotile and HYPERmotile mutants are generally LESS virulent
- potential vaccine strains determined to be LESS reactogenic are LESS motile
3
Q
Who’s genes are doing what?
A
flagellar genes are expressed when TCP and cholera toxin genes are not
4
Q
Tell me about this suggested regulatory network
A
- cells use motility to reach potential colonization site
- once there, turn off motility genes
- and turn on genes required for colonization and toxigenicity
5
Q
What do cells require to adhere to host epithelial cells.
A
surface structures
6
Q
What is TCP
A
(T)oxin (C)o-regulated (P)ilus
7
Q
What is ACF
A
(A)ccessory (C)olonization (F)actors
8
Q
What happens when there are tcp or acf mutations?
A
mutations in tcp or acf genes REDUCE colonization
9
Q
Are they neighbors?
A
acf genes are clustered adjacent to tcp genes
10
Q
What is the TCP-ACF element
A
a large pathogenicity island where both gene sets are located
