Vibrio and related organisms Flashcards

1
Q

What are the general characteristics of Vibrionaceae?

A

Vibrionaceae are gram-negative bacilli, facultative anaerobes, and fermentative bacilli. They are primarily found in water sources and cause gastrointestinal diseases. They have polar flagella and are oxidase positive.

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2
Q

What are the most significant human Vibrios?

A

The most significant human Vibrios are V. cholerae (causes cholera), V. parahaemolyticus, and V. vulnificus.

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3
Q

What is the antigenic structure of V. cholerae?

A

V. cholerae has O lipopolysaccharide antigens that give serological specificity. It also has a single heat-labile flagellar H antigen, which is nonspecific.

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4
Q

What are the serotypes of the O1 serogroup of V. cholerae?

A

The O1 serogroup of V. cholerae is subdivided into three serotypes: Ogawa, Inaba, and Hikojima.

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5
Q

What is the role of the cholera toxin in the pathogenesis of cholera?

A

The cholera toxin is an exotoxin that induces a rise in cAMP inside intestinal epithelial cells, leading to hypersecretion of water and electrolytes (Na+, K+, Cl-, and HCO3-) into the intestinal lumen, causing massive watery diarrhea.

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6
Q

What are the symptoms of cholera?

A

Symptoms of cholera include profuse, watery diarrhea, vomiting, stomach cramps, leg cramps, mild fever, sunken eyes and cheeks, dry mucous membranes, and decreased urinary output.

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7
Q

What is the treatment for cholera?

A

The treatment for cholera includes fluid and electrolyte replacement, acid-base balance adjustment, and antibiotics such as tetracycline.

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8
Q

What is the selective medium for Vibrios, and how does V. cholerae appear on it?

A

The selective medium for Vibrios is Thiosulfate-citrate-bile salts-sucrose agar (TCBS). V. cholerae produces large (2–4 mm in diameter) yellow-colored colonies on TCBS due to sucrose fermentation.

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9
Q

What are the virulence factors of V. cholerae?

A

The virulence factors of V. cholerae include endotoxin (negligible significance), enterotoxin (cholera toxin), adherence factors, mucinase, and vascular permeability factor.

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10
Q

What are the differences between the Classic and El Tor biotypes of V. cholerae?

A

The El Tor biotype produces a hemolysin, is Voges-Proskauer test positive, and is resistant to polymyxin B, while the Classic biotype does not have these characteristics.

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11
Q

What is the role of the string test in identifying V. cholerae?

A

The string test is used to separate Vibrio species from other bacterial species, particularly Aeromonas. All V. cholerae strains are positive in the string test, while Aeromonas strains are negative.

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12
Q

What are the major diseases caused by Aeromonas?

A

The major diseases caused by Aeromonas are gastroenteritis and wound infections. It can also cause opportunistic systemic disease in immunocompromised individuals.

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13
Q

What are the biochemical characteristics of V. cholerae?

A

V. cholerae ferments glucose, maltose, mannitol, and sucrose but not lactose and arabinose. It reduces nitrate, is oxidase positive, indole positive, and halotolerant.

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14
Q

What is the role of mucinase in the pathogenesis of V. cholerae?

A

Mucinase causes extreme cellular desquamation, aiding in the colonization and spread of V. cholerae in the intestinal epithelium.

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