Vesicular Trafficking

Question 1

intracellular trafficking role

Answer 1

move proteins and lipids between different membrane bound compartments

Question 2

involves 3 proteins

Answer 2

GTPases, Adaptors, and Coat proteins

Question 3

Types of GTPases for vesiclular trafficking

Answer 3

ARF and Rab

Question 4

role of GTPases

Answer 4

hydrolyse GTP into GDP- essential role in vesicle formation and transport
via recruiting Coat Proteins to the donor membrane

Question 5

2 Types of Coat protein

Answer 5

COPI and COPII

Question 6

Role of COPI and COPII

Answer 6

COPI- involved in cargo recycling
COPII- Carries cargo from ER-> Golgi

Question 7

Role of Rab GTPases

Answer 7

Regulate vesicular trafficking by- vesicle budding, transport, tethering and fusion

Question 8

specificity of Rab

Answer 8

Each Rab protein is associated with a specific membrane- to ensure correct delivery

Question 9

Role of Adaptor proteins

Answer 9

bridge interaction between Cargo molecules and Coat proteins

Question 10

2 types of adaptor protein

Answer 10

Cargo adaptors- recognise specific cargo molecules

Coat adaptors- recruit coat proteins to donor membrane- dependent on function

Question 12

Vesicle budding (uses which proteins)

Answer 12

Coat proteins and GTPases induce cell curvature
coated vesicle then encapsulates cargo molecules

Question 13

reason for uncoating in proper vesicular trafficking

Answer 13

Coat proteins removed after budding
Allows fusion with target membrane