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Clinical Skills > Venipuncture Collection Tubes > Flashcards

Venipuncture Collection Tubes Flashcards

1
Q

general order of draw

A
  1. blood culture tubes (yellow-black)
  2. non-additive tube (red)
  3. coagulation tubes (light blue)
  4. additive tubes (in this order)
    • SST (red-grey or gold)
    • sodium heparin (dark green or brown)
    • PST (light green)
    • EDTA (lavender)
    • ACDA or ACDB (pale yellow)
    • oxalate/fluoride (light grey)
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2
Q

additive in red top

A

none

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3
Q

MOA of red top

A

blood clots and the serum is separated by centrifugation

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4
Q

uses of red top

A

chemistries, immunology, serology (blood bank/cross match)

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5
Q

additive in gold top

A

none

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6
Q

MOA of gold top

A

serum separator tube (SST) contains a gel at the bottom to separate blood from serum on centrifugation

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7
Q

uses of gold top

A

chemistries, immunology and serology

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8
Q

additive in light green top

A

plasma separating tube (PST) with lithium heparin

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9
Q

MOA of light green top

A

anticoagulates with lithium heparin; plasma is separated with PST gel at the bottom of the tube

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10
Q

use of light green top

A

chemistries

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11
Q

additive of red-gray top

A

SST with clot activator

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12
Q

MOA of red-gray top

A

forms clot quickly and separates serum with SST gel at the bottom of the tube

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13
Q

use of red-gray top

A

chemistries

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14
Q

additive of lavender top

A

EDTA liquid

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15
Q

MOA of lavender top

A

forms calcium salts to remove calcium

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16
Q

uses of lavender top

A

hematology (CBC) and blood bank (crossmatch)

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17
Q

special note for lavender top

A

requires full draw and inversion 8x to prevent clotting and platelet clumping

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18
Q

additive of light blue top

A

sodium citrate

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19
Q

MOA of light blue top

A

forms calcium salts to remove calcium

20
Q

use of light blue top

A

coagulation tests (PT and PTT)

21
Q

special note for light blue top

A

requires full draw

22
Q

additive of dark green top

A

sodium heparin or lithium heparin

23
Q

MOA of dark green top

A

inactivated thrombin and thromboplastin

24
Q

uses for dark green top

A
  1. for lithium level: uses sodium heparin

2. for ammonia level: uses sodium or lithium heparin

25
Q

additive of dark blue top

A

sodium EDTA

26
Q

MOA of dark blue top

A

tubs is designed to contain no contaminating metals

27
Q

uses of dark blue top

A

trace element testing (zinc, copper, lead and mercury) and toxicology

28
Q

additive in large gray top

A

sodium fluoride and potassium oxalate

29
Q

MOA of large gray top

A

actiglycolytic agent preserves glucose up to 5 days

30
Q

use of large gray top

A
  1. for lithium level: uses sodium heparin

2. glucose level requires full draw (hemolysis id short draw)

31
Q

additive of yellow top

A

ACD (acid-citrate-dextrose)

32
Q

MOA of yellow top

A

complement activation

33
Q

use of yellow top

A

HLA tissue typing, paternity testing, DNA studies

34
Q

additive in yellow-black top

A

broth mixture

35
Q

MOA of yellow-black top

A

preserves viability of microbes

36
Q

use of yellow-black top

A

microbiology - aerobes, anaerobes and fungi

37
Q

additive of black top

A

sodium citrate (buffered)

38
Q

MOA of black top

A

forms calcium salts to remove calcium

39
Q

uses of black top

A

Westergren Sedimentation rate

40
Q

special note for black top

A

requires full draw

41
Q

additive in orange top

A

thrombin

42
Q

MOA of orange top

A

quickly clots blood

43
Q

use of orange top

A

STAT serum chemistries

44
Q

additive in brown top

A

sodium heparin

45
Q

MOA of brown top

A

inactivates thrombon and thromboplastin

46
Q

use of brown top

A

serum lead determination

Clinical Skills (2 decks)

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