Vector Info to Know COLD

Question 1

Adenovirus 
Gene Location
Pros
Cons
Insert Capacity

Answer 1

Gene Location:
- Episomal

Pros:

• High Transduction efficiency
• Infects Replicating and Non-replicating cells

Cons:
- Elicits Immune Response

Insert Capacity:
- 8-36 kb

Question 2

Adeno-associated Virus 
Gene Location
Pros
Cons
Insert Capacity

Answer 2

Gene Location:
- Integrates genome into specific region on human chromosome 19

Pros:

• Low Immunogenicity
• Infects both Dividing and non-dividing cells

Cons/Insert Capsity:
- 5 kb is all it can insert

Question 3

Herpesvirus
Gene Location
Pros
Cons
Insert Capacity

Answer 3

Gene Location:
- Episomal

Pros:

• Broad host range
• Infects dividing and non-dividing cells

Cons:
- None listed

Question 4

Liposomes/Naked DNA
Gene Location
Pros
Cons
Insert Capacity

Answer 4

Pros:
- Low Immunogenicity

Cons:
- Poor Levels of Gene Transfer

Question 5

Retrovirus 
Gene Location
Pros
Cons
Insert Capacity

Answer 5

Gene Location:
- Inserts into host genome

Pros:

• Stably Transduces into cells
• Long Term Expression - BECAUSE IT STICKS AROUND THROUGH CELL DIVISION

Cons:

• Does not transduce into non-dividing cells (unless its the lentivirus)
• Inactivated by human complement

Insert Capacity:
8 kb