Vector Facts Flashcards
Which vectors cause an immune response?
Adenoviruses
Herpesviruses
Which vectors transduce a wide range of cells?
Retroviruses Lentiviruses AAV Adenoviruses Herpesviruses
Which vectors infect both non-dividing and dividing cells?
Lentiviruses
AAV
Adenoviruses
Herpesviruses
Which vectors infect only dividing cells?
Retroviruses
Which vectors infect only tumour cells?
Newcastle virus disease
Which vectors integrate into host cell genome?
Retroviruses
Lentiviruses
AAV
Which vectors exist as a non-integrated episome?
Non-viral
Adenoviruses
Herpesviruses
Which vectors have no/low size constraint?
Non-viral
3rd generation Adenoviruses
Herpesviruses
Which vectors have concerns of insertional mutagenesis?
Retrovises
Lentiviruses
AAV
Which vectors can be produced in high titre?
Non-viral Retroviruses Lentiviruses Adenoviruses Non-disabled Herpesviruses
Which conditions are associated with non-viral vectors?
Cystic Fibrosis
Which conditions are associated with retroviruses?
Severe combined immunodeficiency syndrome (SCID)
Brain tumours
Which conditions are associated with lentiviruses?
Neurological disorders Immune deficiencies Lysosomal storage diseases Haemoglobinopathies Cancer
Which conditions are associated with AAV?
Haemophilia
Retinal degeneration
Familial lipoprotein lipase deficiency/severe or multiple pancreatitis attacks
Which conditions are associated with adenoviruses?
Potential for cancer
Ornithine Transcarbamylase Deficiency
Which conditions are associated with Herpesviruses?
Parkinson’s disease
Cancer (ICP34.5)
How are non-viral vectors developed for optimal activity?
Include endosomal disrupting peptide, nucleus localising signal and adenovirus mu protein
How are retroviruses developed for optimal activity?
In cell lines with gag, pol and env in trans.
Human/primate cells are required for packaging cell lines to allow for serum resistance.
Express decay acceleration factors.
Modification of tropism by pseudotyping, chemical modification.
How are lentiviruses developed for optimal activity?
Other animal lentiviruses are being developed which when pseudo typed with VSV-G can infect a range of human cells.
How are AAV developed for optimal activity?
Specific tissue targeting via selective tropism form the 6 human strains.
Chimeric AAV to minimise the effect of neutralising antibodies have
Heterodimersation: to allow for a bigger DNA delivery.
How are adenoviruses developed for optimal activity?
Bispecific conjugates to introduce novel tropism because CAR receptors is widely distributed.
Pseudo typing, different serotypes have different tropisms suggesting an alternative receptor to CAR.
How are herpesviruses developed for optimal activity?
Gamma herpesviruses, won’t be used for gene therapy, express ORF-37 which allows for episomal maintenance in diving cells.
