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ATU 1 > Vector Facts > Flashcards

Vector Facts Flashcards

1
Q

Which vectors cause an immune response?

A

Adenoviruses

Herpesviruses

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2
Q

Which vectors transduce a wide range of cells?

A 
Retroviruses
Lentiviruses
AAV
Adenoviruses
Herpesviruses
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3
Q

Which vectors infect both non-dividing and dividing cells?

A

Lentiviruses
AAV
Adenoviruses
Herpesviruses

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4
Q

Which vectors infect only dividing cells?

A

Retroviruses

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5
Q

Which vectors infect only tumour cells?

A

Newcastle virus disease

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6
Q

Which vectors integrate into host cell genome?

A

Retroviruses
Lentiviruses
AAV

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7
Q

Which vectors exist as a non-integrated episome?

A

Non-viral
Adenoviruses
Herpesviruses

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8
Q

Which vectors have no/low size constraint?

A

Non-viral
3rd generation Adenoviruses
Herpesviruses

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9
Q

Which vectors have concerns of insertional mutagenesis?

A

Retrovises
Lentiviruses
AAV

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10
Q

Which vectors can be produced in high titre?

A 
Non-viral 
Retroviruses
Lentiviruses 
Adenoviruses 
Non-disabled Herpesviruses
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11
Q

Which conditions are associated with non-viral vectors?

A

Cystic Fibrosis

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12
Q

Which conditions are associated with retroviruses?

A

Severe combined immunodeficiency syndrome (SCID)

Brain tumours

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13
Q

Which conditions are associated with lentiviruses?

A 
Neurological disorders 
Immune deficiencies
Lysosomal storage diseases 
Haemoglobinopathies 
Cancer
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14
Q

Which conditions are associated with AAV?

A

Haemophilia
Retinal degeneration
Familial lipoprotein lipase deficiency/severe or multiple pancreatitis attacks

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15
Q

Which conditions are associated with adenoviruses?

A

Potential for cancer

Ornithine Transcarbamylase Deficiency

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16
Q

Which conditions are associated with Herpesviruses?

A

Parkinson’s disease

Cancer (ICP34.5)

17
Q

How are non-viral vectors developed for optimal activity?

A

Include endosomal disrupting peptide, nucleus localising signal and adenovirus mu protein

18
Q

How are retroviruses developed for optimal activity?

A

In cell lines with gag, pol and env in trans.
Human/primate cells are required for packaging cell lines to allow for serum resistance.
Express decay acceleration factors.
Modification of tropism by pseudotyping, chemical modification.

19
Q

How are lentiviruses developed for optimal activity?

A

Other animal lentiviruses are being developed which when pseudo typed with VSV-G can infect a range of human cells.

20
Q

How are AAV developed for optimal activity?

A

Specific tissue targeting via selective tropism form the 6 human strains.

Chimeric AAV to minimise the effect of neutralising antibodies have

Heterodimersation: to allow for a bigger DNA delivery.

21
Q

How are adenoviruses developed for optimal activity?

A

Bispecific conjugates to introduce novel tropism because CAR receptors is widely distributed.

Pseudo typing, different serotypes have different tropisms suggesting an alternative receptor to CAR.

22
Q

How are herpesviruses developed for optimal activity?

A

Gamma herpesviruses, won’t be used for gene therapy, express ORF-37 which allows for episomal maintenance in diving cells.

ATU 1 (4 decks)

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