Validation Flashcards
What do we want from a method?
Consistency (reproducibility)
Reliability (Precision)
Accuracy (Trueness)
Can a systematic error be corrected?
Yes
Can a random error be corrected?
No
What is uncertainty?
An interval within which one believes the correct value is
What is an outlier?
A data point that is completely different from the others
What is QA?
Quality assurance
Systematic measures (quality system) necessary to give enough confidence that a product (results) will fulfill given demands on quality
What is validation?
Ensure that the method is suitable for its intended purpose
Is a specific compound always selective?
Yes
Give an example of selective and specific
Redness and red
Do we always only use the linear part of the curve?
Yes
How do you increase trueness?
Calibration
How do you increase precision?
It is about the tolerance of the equipment you work with. You might need a more precise equipment.
How do we test trueness?
With a t-test
Paired t-test
Linear regression
If we plot residuals against concentration do we want to see random data points or some type of curve?
Random data points to ensure that the linear fit we have fitted is indeed the right fit. If a curve shows up it is not.
What is LOD?
Limit of detection
What is LOQ?
Limit of quantification
Where on the concentration curve do we want to be?
In the middle of the linear interval
What does limit of detection mean?
The measured value is larger than the uncertainty associated with it
What is S/N?
Signal to noise ratio
What does limit of quantification mean?
The lowest amount of analyte that we can quantitatively determine with precision and accuracy
What is robustness?
Capacity of procedure to remain unaffected by small deliberate variations in method parameters
Should the zero concentration blank be included in the calibration curve?
Yes
What is the most common type of weighted regression?
Weighted by the standard deviation
