Validation

Question 1

What do we want from a method?

Answer 1

Consistency (reproducibility)
Reliability (Precision)
Accuracy (Trueness)

Question 2

Can a systematic error be corrected?

Answer 2

Yes

Question 3

Can a random error be corrected?

Answer 3

No

Question 4

What is uncertainty?

Answer 4

An interval within which one believes the correct value is

Question 5

What is an outlier?

Answer 5

A data point that is completely different from the others

Question 6

What is QA?

Answer 6

Quality assurance

Systematic measures (quality system) necessary to give enough confidence that a product (results) will fulfill given demands on quality

Question 7

What is validation?

Answer 7

Ensure that the method is suitable for its intended purpose

Question 8

Is a specific compound always selective?

Answer 8

Yes

Question 9

Give an example of selective and specific

Answer 9

Redness and red

Question 10

Do we always only use the linear part of the curve?

Answer 10

Yes

Question 11

How do you increase trueness?

Answer 11

Calibration

Question 12

How do you increase precision?

Answer 12

It is about the tolerance of the equipment you work with. You might need a more precise equipment.

Question 13

How do we test trueness?

Answer 13

With a t-test
Paired t-test
Linear regression

Question 14

If we plot residuals against concentration do we want to see random data points or some type of curve?

Answer 14

Random data points to ensure that the linear fit we have fitted is indeed the right fit. If a curve shows up it is not.

Question 15

What is LOD?

Answer 15

Limit of detection

Question 16

What is LOQ?

Answer 16

Limit of quantification

Question 17

Where on the concentration curve do we want to be?

Answer 17

In the middle of the linear interval

Question 18

What does limit of detection mean?

Answer 18

The measured value is larger than the uncertainty associated with it

Question 19

What is S/N?

Answer 19

Signal to noise ratio

Question 20

What does limit of quantification mean?

Answer 20

The lowest amount of analyte that we can quantitatively determine with precision and accuracy

Question 21

What is robustness?

Answer 21

Capacity of procedure to remain unaffected by small deliberate variations in method parameters

Question 22

Should the zero concentration blank be included in the calibration curve?

Answer 22

Yes

Question 23

What is the most common type of weighted regression?

Answer 23

Weighted by the standard deviation