UV-VIS SPECTROPHOTOMETRY Flashcards
• Technique used to measure the absorption of ultraviolet and visible light by molecules in a sample
• it provides information about the concentration and structure of molecules based on their absorbance characteristics in the uv invisible regions of the electromagnetic spectrum
UV-Vis spectrophotometry
Basic principle:
____ and ____ comprise only a small part of the electromagnetic spectrum which includes such other forms of radiation as a radio, infrared, cosmic, and x-rays
Ultraviolet and visible radiation
The energy associated with electromagnetic radiation is defined by the equation of:
e = hv, where
e - energy in joules
h- plank constant (6.62 x 10 ^ -34) joul-hertz or joule-second
v- is frequency in seconds
Ultraviolet and visible radiation compromise only a small part of the electromagnetic spectrum which includes such other forms of radiation as radio, infrared, cosmic, and x-rays
THE ELECTROMAGNETIC SPECTRUM
Electromagnetic radiation can be considered as a combination of alternating electric and magnetic fields that travel through space with a wave motion because radiation acts as a wave it can be classified in terms of either ____ or ___
Wavelength or frequency
Wavelength and frequency are related by the equation:
v = c/λ, where
v - frequency in seconds
c - the speed of light (3 * 10 ^ 8 ms-1)
λ- wavelength in meters
Electromagnetic radiation can be considered a combination of alternating electric and magnetic fields that travel through space in a wave motion.
Wavelength and frequency
In general when measuring uv visible spectra we want only ___ to occur. Because light is a form of energy absorption of light by matter causes the energy content of the molecules to ___
Absorbance
Increase
When light passes through or is reflected from a sample the amount of light absorbed is the difference between the ___ __ and ___ ___
incident radiation (Io) and the transmitted radiation (I)
Transmittance equation
T=I/Io or T=I/Io x 100
Absorbance equation
A=-logT
Many spectrophotometers depending on the wavelength setting alternate between a halogen lamp for visible range and the deuterium lamp for the ultraviolet region
Light source
Light source:
Many spectrophotometers depending on the wavelength setting alternate between a ____ lamp for visible range and the ___ lamp for the ultraviolet region
halogen
deuterium
A device that disperses monochromatic from polychromatic light
Monochromator
Monochromator is a device that disperses ___ from ____ light
monochromatic
polychromatic