UV-VIS SPECTROPHOTOMETRY Flashcards

1
Q

• Technique used to measure the absorption of ultraviolet and visible light by molecules in a sample
• it provides information about the concentration and structure of molecules based on their absorbance characteristics in the uv invisible regions of the electromagnetic spectrum

A

UV-Vis spectrophotometry

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2
Q

Basic principle:
____ and ____ comprise only a small part of the electromagnetic spectrum which includes such other forms of radiation as a radio, infrared, cosmic, and x-rays

A

Ultraviolet and visible radiation

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3
Q

The energy associated with electromagnetic radiation is defined by the equation of:

A

e = hv, where
e - energy in joules
h- plank constant (6.62 x 10 ^ -34) joul-hertz or joule-second
v- is frequency in seconds

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4
Q

Ultraviolet and visible radiation compromise only a small part of the electromagnetic spectrum which includes such other forms of radiation as radio, infrared, cosmic, and x-rays

A

THE ELECTROMAGNETIC SPECTRUM

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5
Q

Electromagnetic radiation can be considered as a combination of alternating electric and magnetic fields that travel through space with a wave motion because radiation acts as a wave it can be classified in terms of either ____ or ___

A

Wavelength or frequency

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6
Q

Wavelength and frequency are related by the equation:

A

v = c/λ, where
v - frequency in seconds
c - the speed of light (3 * 10 ^ 8 ms-1)
λ- wavelength in meters

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7
Q

Electromagnetic radiation can be considered a combination of alternating electric and magnetic fields that travel through space in a wave motion.

A

Wavelength and frequency

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8
Q

In general when measuring uv visible spectra we want only ___ to occur. Because light is a form of energy absorption of light by matter causes the energy content of the molecules to ___

A

Absorbance
Increase

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9
Q

When light passes through or is reflected from a sample the amount of light absorbed is the difference between the ___ __ and ___ ___

A

incident radiation (Io) and the transmitted radiation (I)

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10
Q

Transmittance equation

A

T=I/Io or T=I/Io x 100

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11
Q

Absorbance equation

A

A=-logT

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12
Q

Many spectrophotometers depending on the wavelength setting alternate between a halogen lamp for visible range and the deuterium lamp for the ultraviolet region

A

Light source

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13
Q

Light source:
Many spectrophotometers depending on the wavelength setting alternate between a ____ lamp for visible range and the ___ lamp for the ultraviolet region

A

halogen
deuterium

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14
Q

A device that disperses monochromatic from polychromatic light

A

Monochromator

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15
Q

Monochromator is a device that disperses ___ from ____ light

A

monochromatic
polychromatic

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16
Q

is the process of breaking down light into its constituent wavelengths in a manner similar to rainbow

A

Dispersion

17
Q

Tool used to separate the spectral lines

A

Diffraction grating

18
Q

Quartz __ are frequently used for ultraviolet measurement since many plastics and glass absorb uv light and cause interference

A

Cuvette (sample holder)

19
Q

The incoming light is transformed by ___ into a measurable electrical current

A

Detector

20
Q

General components

A

Light source
Monochromator
Cuvette
Detector

21
Q

Journal article

A

Determination of sun protection factor by uv-vis spectrophotometry

22
Q

Objective of the study

A

To determine the real spf value of the chemical or physical sunscreen through uv visible spectroscopy

23
Q

• Quartz is highly ____ to UV light, meaning it does not absorb UV wavelengths.
• This property allows the UV light to pass through the quartz cuvette without significant attenuation or interference

A

Transparent

24
Q

polychromatic light contains ___ wavelengths (colors) of light.

A

Multiple

25
Q

Inside the monochromator, there is a dispersive element, such as a:

A

prism
diffraction grating

26
Q

separates the different wavelengths of light based on their different angles of refraction

A

Prism

27
Q

separates the different wavelengths of light based on their different angles of diffraction

A

diffraction grating

28
Q

After the light is dispersed, it passes through an ____ ___. By moving the slit, the monochromator can isolate a specific wavelength of light, effectively selecting a narrow band of wavelengths (monochromatic light) from the dispersed spectrum.

A

Adjustable slit

29
Q

• the initial amount of light that strikes the sample.
• It represents the intensity of the light source before any interaction with the sample occurs.

A

Incident Radiation (I₀)

30
Q

• the amount of light that passes through the sample and emerges on the other side.
• It represents the intensity of light that is not absorbed by the sample.

A

Transmitted Radiation (I)