Using a microscope .2 Flashcards
What’s A?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit
Eyepiece lens
What’s B?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit
Fine focusing wheel
What’s C?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit
Coarse focusing wheel
What’s D?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit
Objective lens
What’s E?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit
Slide
What’s F?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit
Stage
What’s G?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit
Mirror/ illuminator
What are the steps of using a microscope?
- Always carry it by the handle and the base.
- Begin by turning to the lowest magnification objective lens.
- Place your specimen slide on the stage and clip in place.
- Turn on the light.
5.Turn the coarse focus wheel until you see an image. - If you need a clearer image carefully turn the fine focus wheel
- If you loose focus go back and start again with the large focus wheel.
How do you work out the total magnification?
Magnification of eye piece lens * Magnification of objective lens
How do you work out the magnification of eye piece lens?
Total magnification/ Magnification of objective lense
How do you work out the magnification of the objective lense?
Total magnification/ Magnification of eye piece lense
(Meaning) Staining cells?
Staining cells is when you dye a cell a different colour before putting it under the microscope
Why is it important to stain cells?
Most cells and tissues are see through so you need to stain parts of them with special dyes to make them easier to see under the microscope
How do you look at plant cells under a microscope?
- Cut a small piece of onion
- Use forceps to peel off the very thin inner layer
- Put the piece of onion skin flat on the slide
- Put a few drops of iodine solution onto the onion
- Gently lower the cover slip onto the slide using a mounting needle
(2 reasons) Why do we use coverslips?
-To prevent the specimen from drying
-To flatten the specimen
What solution is used to stain the cell?
Iodine solution
Where is the microscope slide placed?
On the stage
How do you alter the magnification on a microscope?
By changing the objective lens
When preparing a microscope slide, why do you place the cover slip at a 45 degree angle?
To prevent air bubbles
(true/ false) You alter the coarse lens before you alter the fine lens?
True
Which objective lens magnification should you start off with?
The lowest magnification first
