Using a microscope .2

Question 1

What’s A?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit

Answer 1

Eyepiece lens

Question 2

What’s B?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit

Answer 2

Fine focusing wheel

Question 3

What’s C?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit

Answer 3

Coarse focusing wheel

Question 4

What’s D?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit

Answer 4

Objective lens

Question 5

What’s E?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit

Answer 5

Slide

Question 6

What’s F?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit

Answer 6

Stage

Question 7

What’s G?
https://docs.google.com/document/d/1IAIJazXgkb1iAapCoHMhOj-GsGM8JUbwyY3ywHZQ3EY/edit

Answer 7

Mirror/ illuminator

Question 8

What are the steps of using a microscope?

Answer 8

1. Always carry it by the handle and the base.
2. Begin by turning to the lowest magnification objective lens.
3. Place your specimen slide on the stage and clip in place.
4. Turn on the light.
   5.Turn the coarse focus wheel until you see an image.
5. If you need a clearer image carefully turn the fine focus wheel
6. If you loose focus go back and start again with the large focus wheel.

Question 9

How do you work out the total magnification?

Answer 9

Magnification of eye piece lens * Magnification of objective lens

Question 10

How do you work out the magnification of eye piece lens?

Answer 10

Total magnification/ Magnification of objective lense

Question 11

How do you work out the magnification of the objective lense?

Answer 11

Total magnification/ Magnification of eye piece lense

Question 12

(Meaning) Staining cells?

Answer 12

Staining cells is when you dye a cell a different colour before putting it under the microscope

Question 13

Why is it important to stain cells?

Answer 13

Most cells and tissues are see through so you need to stain parts of them with special dyes to make them easier to see under the microscope

Question 14

How do you look at plant cells under a microscope?

Answer 14

1. Cut a small piece of onion
2. Use forceps to peel off the very thin inner layer
3. Put the piece of onion skin flat on the slide
4. Put a few drops of iodine solution onto the onion
5. Gently lower the cover slip onto the slide using a mounting needle

Question 15

(2 reasons) Why do we use coverslips?

Answer 15

-To prevent the specimen from drying
-To flatten the specimen

Question 16

What solution is used to stain the cell?

Answer 16

Iodine solution

Question 17

Where is the microscope slide placed?

Answer 17

On the stage

Question 18

How do you alter the magnification on a microscope?

Answer 18

By changing the objective lens

Question 19

When preparing a microscope slide, why do you place the cover slip at a 45 degree angle?

Answer 19

To prevent air bubbles

Question 20

(true/ false) You alter the coarse lens before you alter the fine lens?

Answer 20

True

Question 21

Which objective lens magnification should you start off with?

Answer 21

The lowest magnification first