Urine Investigation - Glucose conc. - 11 Flashcards
1
Q
Production of a dilution series of a glucose solution and use of colorimetric techniques to produce a calibration curve with which to identify the concentration of glucose in an unknown ‘urine’ Sample
A
2
Q
What is usually the first indication of Diabetes?
A
Sugar in the urine
3
Q
Describe the method used to investigate into urine samples.
A
- Label 6 test tubes from 0-10mmol dm-3
- Dilute the glucose standard (10mol dm-3) with water labelled in the test tubes
- Add 2cm3 of Benedict’s solution to each of the test tubes to each of the test tubes and give it a mix.
- Add 2cm3 of unknown concentration of urine sample and 2cm3 of Benedict’s solution. Do this for both urine samples
- Place the test tubes all in a water bath (including the tubes with the urine samples) and leave for 4 minutes
- Remove test tubes from the water bath and allow to cool
- Using the 0.0mmol dm-3 use as a blank for the colorimeter to zero absorbance
- Using the colorimeter, measure the absorbance of the rest of the test tubes
- Record your results in a table and plot a graph of the absorbance of the known concentrations of glucose.
- Using the graph and the absorbance values obtained for the urine sample, read of from the graph the concentration of glucose in the urine samples.
- Record results in a table
4
Q
What are some of the controls needed to be kept constant in this investigation?
A
- Tempertaure of the water bath - using a thermometer
-Amount of each solution - using syringes - Same colorimeter and is et on the red filter
5
Q
What are some risks associated with this investigation?
A
- Beakers + test tubes - Broken glass
- Hot water bath - Burns
- Solutions - could spill - slip hazard
- Benedict’s solution - irritant - goggles
6
Q
What are the normal ranges of glucose in the urine?
A
0-0.8 mmol dm-3 - A higher concentration could indicate Diabetes
7
Q
MORE Q’S IF POSSIBLE NEED TO BE ADDED
A
