Urinary Tract and Mouth Flashcards
Quantitative analysis
determine the number of bacteria per
mL of urine
Qualitative analysis
examine the features of bacteria that
grow in urine
What is a Calibrated Plate?
With Calibrated loop one streak down the middle and then a streak across the entire plate.
Cariogenic bacteria can cause…
bacteria that can lead to tooth decay
and dental carries (cavities)
- Produce a capsule a stick to teeth
- Ferment sugars to produce acids a dissolve tooth
Species Streptococcus salivarius Sucrose Components?
Glucose= ACID Fructose= CAPSULE
Species Streptococcus mutans Sucrose Components?
Glucose=Capsule
Fructose=ACID
Sucrose plate is…
urinary
differential for capsule
production
colonies appear gooey/
slimy, moist, glistening, dome shaped
Snyder agar is…
urinary
differential for fermentation
Green = (-)
Yellow = (+)
Why are women more likely to get a UTI than men?
- Urethra is shorter in women
2. Anal opening is closer to the urethra in women
10,000-100,000 bac/mL
is considered?
false positive and the patient is
negative for a UTI
What could lead to a false positive?
- Patient didn’t wipe properly before giving sample
- Patient didn’t get a mid-stream catch
- Sample was not tested in a timely manner by the lab
Calibrated loop holds how much fluid?
1uL
What is 1uL in mL
0.001mL
What type of dilution can be made using a calibrated loop?
1x10^3 dilution
How many colonies are required on a calibrated loop to
represent 100,000 bac/mL in the original sample?
100 colonies = UTI
EMB plate is…
- Selective: Gram negative
- Differential: Lactose fermentation
Blood agar plate plate is…
- Selective: nothing
- Differential: hemolysis
Gamma =
no hemolysis
Alpha =
partial hemolysis
Beta =
complete hemolysis
Reducing media…
- Contains thioglycollate which removes oxygen
- E. coli = facultative anaerobe (grows throughout)
Sucrose Plate is…
mouth
- Differential for capsule production
- Look for gooey or sticky looking colonies
Snyder Agar is…
mouth
- Differential for fermentation
- Look for yellow color change à timing of color change
matters! - Determine your risk for dental carries
Risk of dental caries
Timing of Yellow appearance in Snyder Agar
Within 24 hours?
High risk
