Unit two - Metabolism and survival Flashcards
Describe the difference between a reversible and an irreversible step
With a reversible step the product can react to form the original substrate. in am irreversible step the reactants form the product which cannot be reversed.
explain what is meant by an alternative route in a metabolic pathway
when a substrate is acted upon by a different enzyme and follows a different pathway to the end product
name an enzyme which is embedded in the inner mitochondrial membrane
ATP synthase
describe the role of protein pumps embedded in the inner mitochondrial membrane
the use of energy to pump hydrogen ions across the inner mitochondrial membrane
Describe the role of genes in the control of metabolic pathways
each step in a metabolic pathway is controlled by a specific enzyme, each enzyme is coded for by a gene, the order of bases in the gene determine the order of the amino acids, which determines the structure of the protein.
describe the role of the active site of an enzyme during a reaction
The active site has a slightly high affinity for the substrate molecule and a low affinity for the products. this lowers the activation energy of the chemical reaction
Describe the mechanism of induced fit
the enzyme is flexible and so the active site can change shape, the substrate induces the active site to change shape, and the active site can orientate the position of the substrate molecules so they fit better.
describe the effect of an increase in substrate concentration on the direction and rate of an enzyme reaction
increase in substrate concentration drives the chemical reaction in the direction of the end product and increases the rate of reaction
Explain how enzymes speed up the rate of reaction in metabolic pathways
The active site can alter the position of the substrate molecules so that they fit more closely, and the activation energy is lowered when an enzyme is involved
describe the effect of substrate concentration and end- product concentration on a reversible metabolic reaction
increase substrate concentration drives the chemical reaction in the direction of the end product, increase in the end product concentration drive the chemical reaction in the direction of the substrate.
describe the effect of increasing concentration on the rate of reaction in the presence of a competitive inhibitor
reaction rate increases with the substrate concentration, but as substrate concentration increases further there is no further effect on the rate of reaction
describe the effect of increasing substrate concentration on the rate of reaction in the presence of non-competitive inhibitors
inhibitors reduce the rates of reaction nearly to 0 increasing substrate concentration makes no difference.
explain the advantages of end-product inhibition to a cell
stops the production of too much end product
explain the difference between a competitive and a non-competitive inhibitor
competitive inhibitors binds reversibly at the active site, and non-competitive inhibitors binds irreversibly at another binding site.
explain the effect of a competitive inhibitor on the rate of reaction
the rate of the enzyme reaction decreases, and competitive inhibitors have a similar shape to the substrate molecule and attaches at the active site of the enzyme
explain the effect of a non-competitive inhibitor on the rate of reaction
the rate of reaction is reduced to nearly zero and inhibitors cause a change in the shape of the active site so that the substrate cannot bind
Explain why the phosphorylation of intermediates in glycolysis is referred tp as an energy investment stage
Two ATP molecules are used to phosphorylate the intermediates in the energy investment stage, and four ATP molecules are gained in the pay-off stage
Describe the role of the dehydrogenase enzymes in glycolysis and the citric acid cycle
Dehydrogenase enzymes remove hydrogen ions and electrons
Explain the role of ATP during glycolysis
In glycolysis, ATP is used in the phosphorylation of glucose and intermediates
Explain why the ATP produced in glycolysis is referred to as an energy pay-off
Four ATP molecules are produced in the pay-off stage, this gives a net gain of 2 ATP