Unit one Flashcards
What is Linear dilution?
dilutions by equal intervals
What is Log dilution?
dilutions differ by a constant proportion
What does a standard curve do?
determines the unknown
Why is a buffer used?
To control the pH
What is a colorimeter used for?
To quantify concentrations and the turbidity
What does Centrifudge do?
separates substances based in density
what is chromatography used for?
Separating substances
What is affinity chromatography?
method used to separate proteins
How does affinity chromatography work?
Gel colum is created with specific receptors bound to it. As the proteins pass through the column the target proteins become attached
what is gel electrophoresis
process used to separate proteins and nuclei gels
what is the proteome?
entire set of proteins expressed by a genome
why is the proteome larger?
more than one protein can be produced from a single gene
what is included in the mature mRNA transcript?
different combinations of exons
what happens to transcribed DNA
once transcribed the DNA goes through alternative splicing to create a mature transcript. This is then formed into a poly peptide. post translational modifications then take place to form proteins.
what happens when the protein make bonds when changing shape
proteolytic cleavage