UNIT 7 - Microscopic Examination Flashcards
Insoluble substances (formed elements)
○ Redbloodcells (RBCs)
○ Whiteblood cells (WBCs)
○ Epithelial cells
○ Casts
○ Bacteria
○ Yeast
○ Parasites
○ Mucus
○ Spermatozoa
○ Crystals
○ Artifacts
Least standardized, most time-consuming test
Microscopic Examination
CLINICAL AND LABORATORY STANDARDS INSTITUTE (CLSI)
● Requested by the physician
● Laboratory-specified population
● Any abnormal physical or chemical result
● Laboratory criteria are programmed into automated instrumentation
● Preparation of sediment
● Volume of sediment examined
● Methods of visualization
● Reporting of results
Sediment Standardization
Commercial Systems for Sediment Standardization
KOVA
Calibrated centrifuge tubes, special slides to control volume, decanting pipettes, grids for better quantitation
KOVA
Macroscopic Screening Correlations for
COLOR
Blood
Macroscopic Screening Correlations for
CLARITY
Hematuria versus hemoglobinuria / myoglobinuria
Confirm pathologic or nonpathologic cause of turbidity
Macroscopic Screening Correlations for
BLOOD
RBC / RBC Casts
Macroscopic Screening Correlations for
PROTEIN
Casts / Cells
Macroscopic Screening Correlations for
NITRITE
Bacteria / WBCs
Macroscopic Screening Correlations for
LEUKOCYTE ESTERASE
WBCs / WBC Casts / Bacteria
Macroscopic Screening Correlations for
GLUCOSE
Yeast
Routine Specimen Preparation for Microscopic Examination
Refrigeration
■ can cause formation of amorphous
urates/phosphates
■ must be returned to RT first, or
heating, or adding Acetic Acid (can
cause RBC lysis)
Refrigeration
