Unit 6-Cytoskeleton Flashcards
What is the non-polar filament
Intermediate filaments
What does polarity in filaments allow for
Motor protein movement
Which way does Kinesin travel
to + end
Which way does Dynein travel
to - end
Which end of MT grows faster in vitro
+ end
Which end of MT does not grow in vivo and why
- end, attached to centrosome/MTOC
How do beta tubular control dynamic instability
They can hydrolyze GTP -> GDP causing MT disassembly
What is GTP cap
Area that is not experiencing GTP hydrolization
What motor protein is responsible for Golgi placement
Dynein
What motor protein is responsible for vesicle transport
Kinesin
What motor protein is responsible for ER placement
Kinesin
What motor protein is responsible for lysosomal transport
Dynein
What is nucleation
Initial assembly
Taxol stabilizes MT filaments what does this mean?
MT filaments will not depolymerize
Colchicine binds with tubular dimers what does this do?
Prevents MT polymerization
Nocodazole binds with tubular dimers what does this do?
Prevents MT polymerization
Phalloidin stabilizes actin filaments what does this mean?
Prevents depolymerization
Cytochalasin caps PLUS END of actin filaments what does this mean?
Actin filaments will depolymerize at minus end?
Latrunculin binds to g-actin what does this do?
Prevents polymerization
What does ARP 2/3 do?
provides a nucleation site for actin which leads to branching/ movement
What way do most myosin go?
+ end
What actin arrangement do cells have at leading edge of cell
Lamellipodium
What is the consequence of no de-polymerization for actin
No movement because no trailing edge
What is Critical Concentration?
Threshold at which there are enough reactants for the reaction to go forward
What do intermediate filaments do?
Provide mechanical strength to cells
What do microtubules do?
Determine cell shape and organelle placement
