Unit 4 Summary 7 Flashcards
DNA sequencing
DNA sequencing: The determination of the base (nucleotide) sequence of a gene. In one common method, the DNA is cleaved using a restriction enzyme, and the cleaved fragments are subjected to various reactions before being separated by gel electrophoresis. DNA sequencing can be used to determine relationships between individuals of a species, and for determining whole genomes of populations or species.
DNA hybridisation
DNA hybridisation: Formation of a hybrid molecule when single-stranded DNA of two different organisms is brought together and their complementary bases bond together.
Thermal stability
Thermal stability: (Of DNA) Temperature needed to dissociate half of the hybrid molecules formed after bonding the DNA of two different organisms (DNA hybridisation)
Amino acid sequencing
Amino acid sequencing: Method of determining the order of amino acids in a protein
Mitochondrial DNA
Mitochondrial DNA: Circular DNA molecule found in the mitochondria
Molecular clock
Molecular clock: Estimated rate of nucleotide substitution (number of bases per year) for a particular region of DNA; used to estimate number of evolutionary changes.
Phylogeny
Phylogeny: The evolutionary relationships of organisms, usually depicted by a branching tree
Phylogenetic tree
Phylogenetic tree: A phylogenetic tree or evolutionary tree is a branching diagram or “tree” showing the inferred evolutionary relationships among various biological species or other entities—their phylogeny—based upon similarities and differences in their physical or genetic characteristics.
Cladogram
Cladogram: a branching diagram showing the cladistic relationship between a number of species.
Cladistics: Method of building phylogenetic trees based on homologous features that indicate that organisms are related
Speciation
Speciation: The evolution of a new species; for example, as a result of the splitting of an ancestral species by a geographic barrier and subsequent natural selection and/or genetic drift in the separated populations
Allopatric speciation
Allopatric speciation: The physical isolation of populations that begins the process of speciation due to geographic isolation
Geographical isolation
Geographical isolation: Populations of species that are isolated by geographic barriers or distance so that they cannot interbreed; begins the process of allopatric speciation
Selective pressures:
Selective pressures: Limiting factors in the environment of an organism that affects its survival and reproduction
Subspecies
Subspecies: Populations within a species that show genetic differences across a geographic range; also called races
Reproductive isolation
Reproductive isolation: Organisms of different species are not able to interbreed due to behavioural, mechanical or geographic isolations.