Unit 4 Handout Notes

Question 1

History

Jean Denis - 1667

• 1a First What?
• 1b What to What?

Dr. James Blundell - 1818

• 2a First what to what?
• 2b How many survived?

Karl Landsteiner - 1900

-3a Discovered what?

Answer 1

Jean Denis - 1667

• 1a First Blood Transfusion
• 1b Lamb to Human

Dr. James Blundell - 1818

• 2a First human to human transfusion
• 2b 6/10 survived Karl Landsteiner - 1900
• 3a Discovered ABO blood groups

Question 2

1. Who performed first blood tranfusion?
2. What did it involve?

Answer 2

1. Jean Denis - 1667
2. Lamb to Human

Question 3

1. Who performed the first human to human transfusion?
2. How many survived?

Answer 3

1. Dr. James Blundell - 1818
2. 6/10 Survived

Question 4

Who discovered ABO blood groups and when?

Answer 4

Karl Landsteiner - 1900

Question 5

Antigen Expression:

1. ABO - chromosome #?
2. Hh - chromosome #?
3. Sese - chromosome #?

Answer 5

1. ABO - chromosome 9
2. Hh - chromosome 19
3. Sese - chromosome 19

Question 6

A, B and H Antigens on RBC are:

Type ? and ?

Answer 6

A, B and H Antigens on RBC are:

Type 2 and 4 oligosaccharide chains

Question 7

A, B and H soluble antigens/substances in body secretions are:

Type ? and ?

Answer 7

A, B and H soluble antigens/substances in body secretions are:

Type 1 and 3 oligosaccharide chains

Question 8

Gene: H

Enzyme:?

Sugar:?

Product: H antigen

Answer 8

Gene: H

Enzyme: L-fucosyltransferase

Sugar: L-fucose

Product: H antigen

Question 9

Gene: Se

Enzyme:?

Sugar:?

Product: Se substance

Answer 9

Gene: Se

Enzyme: L-fucosyltransferase

Sugar: L-fucose

Product: Se antigen and/or substance

Question 10

Left Side is type?

Right Side is type?

Answer 10

Left Side is type 1

Right Side is type 2

Question 11

Gene: A

Enzyme:?

Sugar?

Product: A antigen and/or A substance

Answer 11

Gene: A

Enzyme: N-acetylgalactoseaminyltransferase

Sugar N-acetylgalactoseamine

Product: A antigen and/or A substance

Question 12

Gene: B

Enzyme:?

Sugar?

Product: B antigen and/or B substance

Answer 12

Gene: B

Enzyme:D-galactosyltransferase

Sugar: D-galactose

Product: B antigen and/or B substance

Question 13

What Antigen is this?

Answer 13

This is an H antigen

Question 14

What antigen is this?

Answer 14

This is an A antigen.

Question 15

What antigen is this?

Answer 15

This is a B antigen

Question 16

How to perform Confirmation of Bombay?

2. What is it?
3. What happens?

Answer 16

Use Ulex europaeus

2. It’s an anti-H lectin
3. No reaction with Bombay cells
   3a. Auto control-own cells and own serum
   - no reaction

Question 17

Antigen Frequencies

1. 0
2. A
3. B
4. AB

Answer 17

Antigen Frequencies

1. 0 (cauc. 45%, af. americans 49%)
2. A (cauc. 40%, af. americans 27%)
3. B (cauc. 11%, af. americans 20%)
4. AB (cauc. 4%, af. americans 4%)