UNIT 4 - Biodiversity and Natural resources Flashcards
What is the plant cell wall mainly made out of?
Cellulose
What is the main function of the plant cell wall?
Support plant cells.
What is the main function of the midlle lamella and where is it found?
It is the outermost layer of the cell and acts as an adhesive, sticking adjacent plant cells together.
What are plasmodesmata and what is their function?
Channels in cell walls that link adjacent cells together for transport of substances and communication between cells.
What are pits and what is their function?
Regions where the cell wall is very thin (in both adjacent cells). It allows transport of substances between cells.
What is an amyloplast and what is its function?
Small organelle enclosed by a membrane. It contains starch granules for starch storage.
What is the function of the vacuole and tonoplast?
The vacuole contains the cell sap (water, enzymes, minerals, water).
Keep the cells turgid - stopping plants from wilting.
Also involved in the breakdown and isolation of unwanted chemicals.
The tonoplast is the surrounding membrane and it controls what enters or leaves the vacuole.
In which two places inside the chloroplast does photosynthesis occur?
Grana and stroma
What is A?
Stroma
What is B?
Thylakoid
What is C?
Granum (plural grana)
(it’s a stack of thylakoid)
What is D?
lamella (plural lamellae)
What is A?
Middle lamella
What is B?
Plasmodesmata
What is C?
Pits
What is the function of xylem vessels?
Transport water and mineral ions and to provide support.
What is the structure of the xylem vessels?
Long, thin structures formed by cells with hollow lumen and no end walls.
This makes uninterrupted tubes thickened with lignin, which adds support.
Water and mineral ions move in and out through pits in the walls where there’s no lignin.
The tubes are found together in bundles.
What is the function of sclerenchyma fibres?
To provide support (not involved in transport).
What is the structure of sclerenchyma fibres?
Like xylem vessels, they are also made of dead, hollow, long cells.
But these cells do have end walls.
Cell walls are thickened in lignin but they don’t contain pits.
They have more cellulose.
What is the function of the phloem?
To transport organic solutes (such as sucrose) from where they are made to where they are needed. (translocation). Isn’t used for support (only transport).
What is the structure of phloem vessels?
Made out of tubes of cells.
Made out of 2 cells: sieve tube elements and companion cells.
Sieve tube elements have sieve plates at the end walls which contain holes to allow solutes to pass through. They have no nucleus, a very thin layer of cytoplasm and few organelles.
There’s 1 companion cell for every sieve tube element - they carry out livng function for both themselves and their sieve cells.
This is a transverse cross section of a stem.
What is A?
Phloem
This is a transverse cross section of a stem.
What is B?
Xylem
This is a transverse cross section of a stem.
What is C?
Schlerenchyma
This is a longitudinal cross section of a stem.
What is A?
Xylem
This is a longitudinal cross section of a stem.
What is B?
Phloem
This is a longitudinal cross section of a stem.
What is C?
Sclerenchyma
How would you dissect plants in order to identify xylem vessels, sieve cells (phloem) and sclerenchyma fibres?
- Cut a thin cross section of the stem (transverse or longitudinal).
- To prevent drying out, use tweezers to place the cut sections in water.
- Stain your cross section with toluidine blue O (TBO) and leave it for 1 minute.
- Rinse off with water and mount it onto a slide.
- Observe through the microscope. TBO stains lignin blue-green so the xylem and sclerenchyma would appear blue-green while the phloem and other tissues without lignin will appear pinkish purple.
What is the chemical structure of starch and how does it relate to its function?
It’s made from amylose and amylopectin - two alpha glucose chains. (cells get energy for glucose)
Amylose is a long, unbranched chain - this makes it compact, so good for storage.
Amylopectin is a branched chain - the side branches allow enzymes to release glucose quicker.
Starch is insoluble, so it won’t cause water to enter cells by osmosis, preventing them from swelling.
What is the chemical structure of cellulose and how does it relate to its structure?
Long, unbranched chains of beta-glucose, joined by 1,4 straight glycosidic bonds.
Hydrogen bonds join between 50 and 80 cellulose chains together, forming microfibrils.
They are strong so provide structural support.
What are the two main reasons why plant fibres are strong?
- The net-like arrangement of cellulose microfibrils and their strength.
- Secondary cell wall (between cell wall and membrane) contains more lignin and is thicker. Some structural plant cells grow it after finishing their growth - secondary thickening.
How can you measure the tensile strength of plant fibres?
- Attach the fibre to a clamp stand and hang a weight from the other end.
- Keep adding weights until the fibre breaks. Record the mass needed to break it.
- Repeat the experiemnts with different samples of the same fibres and same length. Variables (temp, humidity) should be kept constant.
- Calculate mean of results.
- Safety: wear goggles, be careful with the masses.
What is sustainability?
Using the resources to meet the needs of the present without sacrificing the needs of future generations.
What are renewable resources?
Resources that can be used indefinetly without running out.
How can using plant fibres contribute to sustainability?
- Substitutes plastic, which is made form oil, so not renewable.
- Biodegradable
- Plants are easier to grow and process than oil. They are cheaper and easier to do in developing countries. (less technology is needed).
How can using starch contributeto sustainability?
- Can make bioplastics.
- Can make biofuels i.e. bioethanol.
- This substitutes the use of fossil fuels such as oil and therefore increases sustainability.
For what do plants need water?
- Photosynthesis
- Transporting minerals
- Structural rigidity (turgor pressure)
- Regulate temperature (evaporating water helps plant cool down)
For what do plants need magnesium ions?
Production of chlorophyll - the pigment needed for photosynthesis.
For what do plants need nitrate ions?
Production of DNA, proteins and chlorophyll.
For what do plants need calcium ions?
Important in cell walls - they are required for plant growth.
(calcium pectate)
How could you investigate the effect of calcium ions in plants?
- Make 3 nutrient broths of varying concentrations of calcium ions.
- Use three test tubes for each broth.
- Take 9 seedlings of the same plant and of the same age. Measure the initial mass of the seeds. Use a cotton wool to put the seedlings in the broth so that te roots are suspended.
- Cover in aluminium foil so that no other organisms use light to develop in te nuttrient broth.
- Place all tubes near a light source for a specific amount of time (2 weeks).
- Remove the plant and blot it dry. Measure new mass. Calculate the change in mass. Note down visual differences too.
How can you investigate the antimicrobial properties of some plants using aseptic techniques?
- Prepare and agar plate of bacteria. Use a sterile pippette to transfer bacteria from the broth (water+bacteria+nutrients) to the agar plate.
- Prepare the extract of the plants. Dry, grind, and soak the plant in ethanol (which would extract the antimicrobial substances, which are soluble in ethanol). Filter.
- Dip equally sized discs of sterile absorbent paper in the plant extract. The control will only be soaked in ethanol.
- Place the paper discs on the agar plate away from each other. Place the lid and incubate at 25ºC for 24-48 hours. (temp. is high enough for bacteria to grow well but low to prevent growth of unwanted pathogens).
- Keep all other variables constant.
- There’ll be a clear zone where bacteria can’t grow.
- The larger the clear zone, the more antimicrobial the plant is: work out the area (Pi.r2) or diameter.
What are examples of conditions needed for bacterial growth?
- Nutrients
- Oxygen (if aerobic respiration)
- Temperature and pH (would alter enzyme activity and therefore metabolic processes).
Why are aseptic techniques used?
To prevent contamination of unwanted organisms and human pathogens, since it can affect the growth of the microorganism of interest.
Which aseptic techniques should be used when testing the antimicrobial properties of plants?
- Close windows+doors.
- Regularly disinfect work surfaces to minimise contamination.
- Use sterile equipment and discard it safely. Galssware can be sterilised in an autoclave (steams equipment at high pressure).
- Work near a bunsen flame when transferring bacteria. Hot air rises so microbes in the air won’t land in the plate. (ethanol is flammable so be careful).
- Flame the neck of the broth-containing container when opened and closed to cause air to move out, preventing unwanted organisms from falling in.
Who was William Withering and what did he discover?
- William Withering was a scientist in the 1700s that discovered of foxgloves could treat dropsy (swelling caused by heart failure). The extract contained the drug digitalis.
How did William withering test his digitalis soup?
- Chance observation: he observed that a patient recovered well from dropsy after being treated.
- He knew foxgloves were poisonous so he discovered by trial and error the right amount. (too little had no effect, too much poisoned).
How is a drug tested before clinical trials are carried out on humans?
- Computer modelling for potential effects.
- Tests carried out on human tissues in a lab.
- Tested on live animals.
Modern drug testing in humans consist of 3 phases.
What does phase 1 consist on?
Testing the drug on a small group of healthy individuals to find safe dosage, side effects and body reaction to the drug.
Modern drug testing in humans consist of 3 phases.
What does phase 2 consist on?
Once the drug passes phase 1, it will be tested on a larger group of people (this time PATIENTS) to see how well the drug works.
Modern drug testing in humans consist of 3 phases.
What does phase 3 consist on?
Once the drug has passed phases 1 and 2, the drug is compared to existing treatments. It is tested on hundreds or thousands of patients (to increase reliability). Some patients recieve the existing drug while others recieve the new drug. This is to see if the new drug is actually better than the existing ones.
What are two methods that increase validity in modern drug testing?
- The use of placebos (phase 2).
- Double blind study design (phase 2 and 3).