Unit 2 Test Flashcards
What are the parts of the nucleotide building block
The nitrogenous base, a sugar molecule (deoxyribose), and a phosphate.
Base pairing-pyrimidines and purines
Chargaff’s rule: A-T and C-G, Adenine and Guanine are purines, Thymine and Cytosine are pyrimidines.
Parts of the double helix
Deoxyribose and phosphate alternating (in the actual helix).
The Polymerase role
Builds the new DNA strand (by reading the parent strand and matching nucleotides).
Ligase role
Glues okazaki fragments on the lagging strand together.
Helicase role
Unwinds and unzips the double stranded DNA via breaking the hydrogen bonds between bases.
Unzipping is when
helicase breaks the hydrogen bonds
The Replication fork is
the shape the unzipped DNA makes
Copying is
polymerase recognizes the primer in which primase prepares and uses free nucleotides to build the new strand via parent code.
Base pairing
adenine (A), cytosine (C), guanine (G), thymine (T)… A = T and C = G
How many bonds do Cytosine and guanine have?
3 hydrogen bonds
How many bonds do Adenine and thymine have?
2 hydrogen bonds
What direction is the leading strand made in?
(3’ to 5’) as the moving fork
What direction is the lagging strand made in?
(5’ to 3’) in short Okazaki fragments.
Okazaki Fragments
Short synthesized DNA sequences that get put together to make the lagging strand of DNA.