Unit 2 lab

Question 1

In Entamoeba histolytica, what is the size of trophozoite?

Answer 1

10-60um (ave: 15-20um)

Question 2

In Entamoeba histolytica, what is the size of cyst?

Answer 2

10-20um(12-15um)

Question 3

It has a motility and pseudopodia that are progressive, rapid, unidirectional motility; with hyaline, fingerlike pseudopodia formed rapidly

Answer 3

Trophozoite

Question 4

How many Nucleus does the Entamoeba histolytica trophozoite had?

Answer 4

1

Question 5

What is the cytoplasm appearance of trophozoite has?

Answer 5

Finely granular, Ground glass appearance

Question 6

Shape of Cyst

Answer 6

Usually spherical

Question 7

Trophozoite of E. histolytica/E. dispar in a direct wet mount stained with

Answer 7

iodine

Question 8

Trophozoite of E. histolytica with ingested erythrocytes stained with

Answer 8

trichrome

Question 9

How many nucleus that cyst had?

Answer 9

1-4

Question 10

Mature cyst contains how many nuclei?

Answer 10

4

Question 11

Usually elongate with blunt, rounded, smooth ends (cigar-shaped); may be round or oval

Answer 11

Chromatoidal bodies

Question 12

glycogen vacuole may be present, usually diffuse, stains _____ ______ with _____.

Answer 12

reddish brown; iodine

Question 13

How many specimen do we need if the patient has intestinal amoebiasis?

Answer 13

6 specimens

Question 14

Trophozoites can be seen in what type of stool sample?

Answer 14

Watery

Question 15

Cyst can be seen in what type of stool sample?

Answer 15

Formed

Question 16

Bacteria in Amoebic dysentery? (Few/Numerous)

Answer 16

Few

Question 17

Pus cellsnin Amoebic Dysentery

Answer 17

Scanty, well-preserved

Question 18

Charcot-Layden Crystals is may be present in:

Answer 18

Amoebic Dysentery

Question 19

Trophozoites are absent in:

Answer 19

Bacillary Dysentery

Question 20

Macrophage in Bacillary Dysentery

Answer 20

May be numerous(may have RBC)

Question 21

color of Trophozoites’ cytoplasm in Trichrome

Answer 21

Blue-green, sometimes light pink or with a tinge of purple

Question 22

They are Blue-Gray color in Iron Hematoxylin

Answer 22

Cytoplasm of Trophozoites & Cysts

Question 23

It is slightly more purple in Trichrome

Answer 23

Cyst

Question 24

In Trichrome, what is the color of Nuclei/karyosome, RBC, chromatoidal bodies, Charcot-Leyden crystals?

Answer 24

Red, sometimes with a tinge of purple

Question 25

In Iron Hematoxylin, what is the color of Nuclei/karyosome, RBC, chromatoidal bodies, Charcot-Leyden crystals?

Answer 25

Darker than cytoplasm, blue/gray to black

Question 26

It has a green, and provide nice contrast with protozoa

Answer 26

Trichrome

Question 27

It has a lighter shade of blue-gray in background material

Answer 27

Iron Hematoxylin