Unit 2 lab Flashcards
In Entamoeba histolytica, what is the size of trophozoite?
10-60um (ave: 15-20um)
In Entamoeba histolytica, what is the size of cyst?
10-20um(12-15um)
It has a motility and pseudopodia that are progressive, rapid, unidirectional motility; with hyaline, fingerlike pseudopodia formed rapidly
Trophozoite
How many Nucleus does the Entamoeba histolytica trophozoite had?
1
What is the cytoplasm appearance of trophozoite has?
Finely granular, Ground glass appearance
Shape of Cyst
Usually spherical
Trophozoite of E. histolytica/E. dispar in a direct wet mount stained with
iodine
Trophozoite of E. histolytica with ingested erythrocytes stained with
trichrome
How many nucleus that cyst had?
1-4
Mature cyst contains how many nuclei?
4
Usually elongate with blunt, rounded, smooth ends (cigar-shaped); may be round or oval
Chromatoidal bodies
glycogen vacuole may be present, usually diffuse, stains _____ ______ with _____.
reddish brown; iodine
How many specimen do we need if the patient has intestinal amoebiasis?
6 specimens
Trophozoites can be seen in what type of stool sample?
Watery
Cyst can be seen in what type of stool sample?
Formed
Bacteria in Amoebic dysentery? (Few/Numerous)
Few
Pus cellsnin Amoebic Dysentery
Scanty, well-preserved
Charcot-Layden Crystals is may be present in:
Amoebic Dysentery
Trophozoites are absent in:
Bacillary Dysentery
Macrophage in Bacillary Dysentery
May be numerous(may have RBC)
color of Trophozoites’ cytoplasm in Trichrome
Blue-green, sometimes light pink or with a tinge of purple
They are Blue-Gray color in Iron Hematoxylin
Cytoplasm of Trophozoites & Cysts
It is slightly more purple in Trichrome
Cyst
In Trichrome, what is the color of Nuclei/karyosome, RBC, chromatoidal bodies, Charcot-Leyden crystals?
Red, sometimes with a tinge of purple
In Iron Hematoxylin, what is the color of Nuclei/karyosome, RBC, chromatoidal bodies, Charcot-Leyden crystals?
Darker than cytoplasm, blue/gray to black
It has a green, and provide nice contrast with protozoa
Trichrome
It has a lighter shade of blue-gray in background material
Iron Hematoxylin
