Unit 2 Checkpoint Questions Flashcards
What factor does RNA polymerase require to bind to the promoter in prokaryotic transcription?
Sigma factor
Which type of transcription termination is coupled to polyadenylation?
Eukaryotic transcription
What elements are found in prokaryotic gene promoters?
Pribnow box and -35 segment
How many different types of RNA polymerases perform eukaryotic transcription?
Three different types
What protein may be utilized for termination in prokaryotic transcription?
Rho protein
What element is found in eukaryotic gene promoters?
TATA box
How many types of RNA polymerase generally perform prokaryotic transcription?
Single type
Which transcription factor is RNA polymerase II recruited by?
TFIIB
What is a characteristic of promoters in both prokaryotic and eukaryotic transcription?
Rich in A and T nucleotides
What modification may occur to the RNA product in eukaryotic transcription?
RNA product may be modified
What powers synthesis in both prokaryotic and eukaryotic transcription?
Cleavage of phosphates from NTPs
What transcribes protein-coding genes in eukaryotes?
RNA polymerase II
What is the first general transcription factor to bind to DNA during eukaryotic transcription initiation?
TFIID
What binds to the rut site of a transcript upstream of a stem loop?
Rho protein
What is necessary for processing and makes internal cuts on an RNA transcript?
Endonuclease
What performs splicing of eukaryotic protein-coding mRNA?
Splicosome
What creates an open complex on eukaryotic DNA and phosphorylates RNA polymerase II to activate it?
TFIIH
Which of the following would be considered a cis-regulatory element? Select all that apply.
Proximal promoter element, Distal promoter element, Promoter
