Unit 2 - Cells and Tissue Flashcards

1
Q

Describe the difference in internal membranes between pro/eukaryotic cells

A

prokaryotic - internal membranes are not common, except for photosynthetic bacteria that have internal membranes specialised for harnessing light energy
eukaryotic - internal array of membranes that define the organelles that divide the cell into smaller spaces specialised for different functions

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2
Q

difference between cytosol and cytoplasm

A

cytoplasm - the entire contents of a cell other than the nucleus
cytosol - jelly like internal environment that surrounds the organelles

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3
Q

Difference in plant and animal organelles

A

Animal - nucleus, endoplasmic reticulum, golgi apparatus, lysosomes
Plant - cell wall, vacuoles for water uptake, chloroplasts for energy conversion, plasmodesmata (channels allowing the passage of large molecules such as mRNA and proteins between neighbouring cells)

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4
Q

are organelles isolated entities

A

no. the are either physically connected by membrane bridges or transiently connected by vesicles (small membrane enclosed scas that transport substances within a cell or from the interior to the exterior of the cell

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5
Q

how do vesicles form

A

by budding off an organelle, taking with them a piece of the membrane and internal contents of the organelle that they derived from. they then fuse with another organelle of the cell membrane, reforming a continuous membrane and unloading their contents

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6
Q

What does the endomembrane system consist of

A

the nuclear envelope, ER, golgi apparatus, lysosomes, cell membrane, vesicles

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7
Q

what is specific to plants concerning the endomembrane system

A

The endomembrane system is continuous between cells through plasmodesmata

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8
Q

Define 1. exocytosis and 2. endocytosis

A
  1. when a vesicle fuses with the cell membrane, providing the vesicle with a way to empty its contents to the extracellular matrix or deliver proteins embedded in the vesicle membrane to the cell membrane
  2. budding off from the cell membrane and bringing enclosed material from the outside of the cell into the cell interior
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9
Q

nuclear membrane

A

double membrane that surrounds the nucleus, containing nuclear pores

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10
Q

nuclear pores

A

openings in the nuclear envelope that transport proteins (transcription factors) into the nucleus and mRNA out of the nucleus to the cytosol - the mRNA binds to ribosomes or stay free in the cytosol -

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11
Q

where does protein synthesis occur

A

in the cytosol, free ribosomes in cytosol associated with the ER

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12
Q

Endoplasmic Reticulum

A

Physically continuous with the nuclear envelope, interior (lumen) is continuous throughout. very convoluted membrane for larger SA
involved in protein and lipid synthesis.
produces and transports many of the proteins and lipids used inside and outside the cell (including transmembrane proteins)

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13
Q

Rough ER

A

has ribosomes
synthesizes transmembrane proteins, proteins that end up in the interior of the organelles and proteins destined for secretion
cells that secrete large quantities of proteins have extensive rough ER (eg cells of the gut that secrete digestive enzymes, and cells of the pancreas that secrete insulin)

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14
Q

Smooth ER

A

lacks ribosomes
site of fatty acid and phospholipid biosynthesis
more common in cells that produce lipids (eg cells that synthesize steroid hormone, as they produce cholesterol)

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15
Q

Golgi Apparatus

A

three primary roles:
1. further modifying proteins (with enzymes) and lipids produced by the ER
2. acts as a sorting station as these proteins and lipids move to their final destinations
3. the major site where carbohydrates are added to proteins and lipids
made up of flattened membrane sacs called cisternae
vesicles are the primary way that proteins move through the golgi apparatus with to their final destination

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16
Q

Where do Lysosomes originate from

A

They are specialised vesicles derived from the golgi apparatus

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17
Q

What is the function of lysosomes

A

They degrade damaged or unneeded macromolecules. therefore playing a key role in intracellular digestion and the recycling of organic compounds. they’re also involved in apoptosis (programmed cell death)

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18
Q

How do lysosomes break down molecules

A

They contain a variety of hydrolytic enzymes that are packaged into lysosomes by the golgi apparatus. The golgi apparatus also packages the macromolecules that are destined for degradation

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19
Q

what is the optimal internal environment of proton pumps

A

pH 5 (acidic)

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20
Q

How does the cell protect the organelles from being degraded by the lysosomes

A

By having an environment of pH 7, in which the lysosomal enzymes cannot function

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21
Q

What do mitochondria do

A

harness energy from chemical compounds such as sugars and convert it into ATP. A proton electrochemical gradient is used to synthesize ATP for use by the cell

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22
Q

Describe the structure of mitochondria

A

Two membranes, an outer membrane and a highly convoluted inner membrane whose folds project into the interior.
These folds allow for a greater surface area available for the biochemical machinery that pumps protons and then synthesizes ATP

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23
Q

What do chloroplasts do

A

capture the energy of sunlight to synthesize simple sugars (this process is called photosynthesis) results in the release of O2

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24
Q

describe the structure of chloroplasts

A

surrounded by a double membrane, and have a third, internal membrane, called the thylakoid membrane

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25
Q

What does the thylakoid membrane do

A

it separates the internal compartment called the thylakoid, the membrane contains specialised light collecting molecules called pigments (chlorophyll)

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26
Q

describe the function of chlorophyll

A

collects light energy, which enzymes in the chloroplast then use CO2 as a carbon source for carbohydrates

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27
Q

What are the functions of vacuoles in animal and plant cells

A

animal - help get rid of waste products
plants - help maintain water balance

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28
Q

What roll does vacuoles play in homeostasis

A

They regulate the turgidity by regulating the amount of water in a cell. they will absorb water and diffuse it out of the cell if it is in excess, if the cells lacks water the vacuole tends to revert it back into the cell

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29
Q

What four types of tissue do animals have

A
  1. epithelial
  2. connective
  3. nervous
  4. muscle

they combine to form organs

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30
Q

What determines the shapes of cells in animals

A

Maintained and determined by structural protein networks in the cytoplasm called the cytoskeleton

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31
Q

What are cell junctions

A

complexes of proteins in the cell membrane where a cell makes contact with another cell or the extracellular matrix

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32
Q

what is the extracellular matrix and what is its importance

A

A meshwork of proteins and polysaccharides outside of many cells and tissues. It are important in maintaining a strong, properly shaped tissue or organ

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33
Q

What is endosymbiotic theory

A

The theory states that the mitochondria and chloroplast in eukaryotic cells were once aerobic bacteria (prokaryote) that were ingested by a large anaerobic bacteria (prokaryote). This theory explains the origin of eukaryotic cells.

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34
Q

What is the main role of the cytoskeleton

A

Provides internal support for the cellsh

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35
Q

What two cytoskeletal elements do all eukaryotes have

A

microfilaments and microtubules (animals cells also have a third element, intermediate filaments)

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36
Q

What are these filaments made of

A

long chains, or polymers, made up of protein subunits

37
Q

what are the functions of the microtubules, and microfilaments

A

structural support, and enabling cells to change shape, move about, and transport substances

38
Q

which part of the cytoskeleton is the smallest

A

the microfilaments (7nm diameter)

39
Q

What are microfilaments made of and what do they do

A

Actin monomers, arranged to form a helix.
- extensively branched in the area just below the cell membrane, reinforcing this area (the cell cortex) and helping to organize proteins associated with it
- play important role in maintaining cells shape

40
Q

what is the main function of intermediate filaments and what allows them to do this

A

providing the cell with mechanical strength - they are polymers made of intermediate filament proteins that combine to form a strong cable like structure

41
Q

what are intermediate filaments made from

A

it varies
epithelial - keratins
fibroblasts - vimentins
neurons - neurofilaments

42
Q

what is the largest of the 3 cytoskeletal elements

A

microtubules

43
Q

what are microtubules made from

A

protein dimers (each made up of two different tubulin proteins) alpha and beta tubulin

44
Q

What specific structure are the microtubules connected to in animal cells

A

the centrosome

45
Q

what is the function of microtubules

A

help maintain cell shape and internal structure

46
Q

what is dynamic instability and why does it occur

A

the process of microtubules undergoing seemingly random cycles of repaid depolymerization and then by slower polymerization - this allows spindle microtubules to quickly find and attach to chromosomes during cell division

47
Q

difference in plus and minus end of microtubules

A

plus end undergoes rapid polymerization

minus end undergoes slow polymerization

48
Q

what else assists microtubules in the depolymerization/polymerization

A

motor proteins

49
Q

which motor proteins are associated with which element of the cytoskeleton

A
  1. Microfilaments = myosin
  2. Microtubules = kinesin & dynein
50
Q

what do the different motor proteins do

A
  1. myosin - transport cellular cargo (such as vesicles), also responsible for changes in shape of many types of cells (contracting of muscle cells - which depends on the interaction of myosin with microfilaments and powered by ATP)
  2. kinesin - transports cargo toward the plus end of the microtubules, located at the periphery of the cell
  3. dynein - transports cargo away from the cell membrane toward the minus end, located at the centrosome in the interior of the cell
51
Q

what drives the kinesin and dynein

A

driven by conformational changes in the motor proteins and powered by energy harvested from ATP

52
Q

give an example of kinesin and dynein working

A

colour change in zebrafish embryos. melanin granules are redistributed along microtubules in the melanophores of the skin, driven by kinesin and dynein

53
Q

what are cilia

A

rod-like structures that extend from the surface of cells and are well conserved across the eukaryot

54
Q

What are the functions of nonmotile cilia and give an example

A

sensory function - taking in the environmental signals and transducing them to the cell interior

eg. found in mammalian olfactory neurons in the nose and photoreceptors in the eyes

55
Q

what is the function of motile cilia and give an example

A

to propel the movement of cells or fluid surrounding the cell

eg. green algae and paramecium, where cilia propel the organisms through water. and in sperm cells (flagella)

56
Q

what role does cilia play in protecting us from pathogens

A

they are present in epithelial cells (line the supper respiratory tract) where they move the fluid above the surface of the cell layer, carrying away foreign particles and pathogens

57
Q

what motor protein is associated with the movement of cilia

A

microtubules associate with dynein, which causes the microtubules to bend, causing a wavelike motion of the cilia

58
Q

What are cell adhesion molecules

A

proteins on the surface of cells that recognize and attach cells to each other or to the extracellular matrix

59
Q

what is the most important example of a cell adhesion molecule

A

cadherins (calcium-dependent adherence proteins)

59
Q

what kind of proteins are cadherins

A

transmembrane proteins

60
Q

What do cadherins connect together, what does this result in and how do they do this

A
  1. they connect the cytoskeleton of one cell to the cytoskeleton of another
  2. increasing the strength of tissues and organs
  3. the extracellular domain of a cadherin binds to the extracellular domain of a cadherin of the same type on an adjacent cell. the intracellular domain of the cadherin is linked to the cytoskeleton (including microfilaments and intermediate filaments)
61
Q

what are integrins

A

they are transmembrane proteins, and cell adhesion molecules that allow cells to attach to proteins of the extracellular matrix.
they also act as receptors that communicate info about the extracellular matrix to the interior of the cell.

62
Q

What are the two types of anchoring cell junctions

A

adherens junctions and desmosomes

63
Q

What cell junction are microfilaments attached the cell membrane and epithelial cells with

A

adherens junction

64
Q

what is the difference between adherens and desmosomes

A

adherens - form a belt around the circumference of cells
desmosomes - buttonlike points of adhesion

65
Q

what is the specific role of tight junctions

A

they prevent materials from passing freely through the spaces between the cells - they establish a seal between cells that prevents molecules from moving through the channel

66
Q

What makes up tight junctions

A

integral membrane proteins (claudins and occludins)

67
Q

what two regions does a tight junction divide the cell membrane into

A

apical - which is in contact with the lumen
basolateral - the bottom and sides of the cell

68
Q

what causes the different regions of tight junctions to have different functions

A

the fact that they have different integral membrane proteins

69
Q

what other function can cell junctions have, give examples of these junctions

A

permitting materials to pass directly from the cytoplasm of one cell to the cytoplasm of another, allowing cells to communicate with each other.
gap junctions (animal cells) and plasmodesmata (plant cells)

70
Q

what makes up gap junctions

A

a complex of integral membrane proteins called connexins arranged in a ring

71
Q

what allows/causes the movement of molecules through these gap junctions

A

ions and signaling molecules

72
Q

What are plasmodesmata and what are their functions

A

passages through the cell walls of adjacent plant cells. they allow cells to exchange ions and small molecules

73
Q

what is the size of plasmodesmata for

A

large enough for cells to transfer RNA molecules and proteins

74
Q

What is the function of the extracellular matrix

A

provides a molecular framework that helps determine the structural architecture of plants and animals. provides structural support and info cues that determine the activity of the cells that are in contact with it

75
Q

what is the extracellular matrix composed of

A

proteins, polysaccharidesh

76
Q

what is the animal extracellular matrix composed of

A

large fibrous proteins, including collagen, elastin, laminin (strength)

77
Q

how does the matrix protect against compression and other physical stress

A

the matrix is negatively charged, attracting positively charged ions and water molecules that provide this protection

78
Q

what are the two functions of connective tissue

A
  1. physically connects various parts of the body - eg. tendons that connect your muscles to bones, or ligaments that connect your bones to other bones
  2. supports various parts of the body (underlies all epithelial tissue) - eg. dermis of the skin is connective tissue, that provides support and nutrients to the overlying epidermis
79
Q

what role does the extracellular matrix play in synthesizing of connective tissue

A

it determines the properties of differnt connective tissue due to being dominant in the tissue

80
Q

which cell in the dermis synthesizes most of the extracellular matrix

A

fibroblasts

81
Q

what is the most abundant protein in the extracellular matrix of animals

A

collagen (25% of all the proteins in our body)

82
Q

what type of collagen is the most abundant in our body

A

Type I collagen found in the dermis of the skin

83
Q

what does type I collagen do

A

provides strong, durable support for the epidermis

84
Q

what gives collagen the ability to provide strength

A

its rope like structure, it is composed of intertwined fibres that makes it much stronger than a single fibre of the same diameter

85
Q

What is the extracellular matrix in plants

A

it forms the cell wall

86
Q

what is the main component of of the plant cell wall

A

polysaccharide cellulose

87
Q

what is the function of cell walls in plants

A

maintains the shape and turgor pressure of plant cells and acts as a barrier that prevents foreign materials and pathogens from reaching the cell membrane

88
Q

what are the 3 layers making up the plant cell wall

A
  1. Outermost Middle Lamella - synthesized first (during late stage cell division) & composed of a glue-like complex carbohydrate, which is the main mechanism by which plant cells adhere to one another
  2. Primary Cell Wall - laid down while the cells are still growing, consisting of mainly cellulose (but also others, eg. pectin) & assembled by enzymes on the cell surface, remains flexible and thin
  3. Secondary Cell Wall - constructed when cell growth has stopped, located closest to the cell membrane, made largely of cellulose (but also contains lignin - the lignin hardens the cell wall, making it water resistant), & in woody plants the cell wall can be as much as 25% lignin, allowing them to grow to great heights