Unit 11

Question 1

what is the principle behind field flow fractionation?

Answer 1

separation field vs forces of diffusion

distribution of the particles in the different layers of the parabolic flow is greater than separation

Question 2

the crossflow separation field is perpendicular to what?

Answer 2

the channel

Question 3

what are examples of two configurations of field flow fractionation?

Answer 3

asymmetric flow field flow fractionation (AF4)

hollow fiber flow field flow fractionation (HF5)

Question 4

what is a fractogram?

Answer 4

diagram representing the dector signal vs time during the FF separation

Question 5

what are advantages of field flow fractionation over other separation techniques?

Answer 5

• no stationary phase (no sample breakthrough effects of sample loss)
• upper limit of FF extends to 10^16 Da or wide dynamic particle size range is achievable
• works on aqueous and organic solvent carriers (versatile in terms of density, solubility ,etc…)
• mild operation conditions (good for fragile analytes)
• particles can be measured in dispersion without shape change

Question 6

define omics

Answer 6

the analysis of interactions of biological information objects in various “omes”

Question 7

what are main focuses of omics?

Answer 7

1. mapping information objects (eg. genes, proteins, ligands)
2. finding interaction relationships among objects
3. engineering networks and objects to understand and manipulate regulatory mechanisms
4. integrating omes and omic subfields

Question 8

define foodomics

Answer 8

study of food domains in application of omic technologies to improve consumers well-being, health and confidence

Question 9

define metabolomics

Answer 9

study of global changes in the entire metabolite set of certain cells, tissues, organs and organisms

applied in fields such as medical, environment, food science

Question 10

describe the workflow of a typical metabolomics

Answer 10

1. sample prep to extract the metabolome
2. screening of the extract with advanced analytical tools
3. data trt
4. interpretation of results

Question 11

what are examples of applications of foodomics?

Answer 11

1. identification of prohibited substances
2. characterization of GM foods
3. discovery of new dietary markers
4. food authenticity