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Microbiology Lab > Unit 10 Practice Quizzes > Flashcards

Unit 10 Practice Quizzes Flashcards

1
Q

A red ring on the top of SIM media is positive for the enzyme

A) tryptophanase
B) deaminaase
C) decarboxylase
D) cysteine desulfhydrase

A

A) tryptophanase

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2
Q

If the urease tube is all pink it is positive for urease. If it is pink on the slant only it is negative for urease.

A) True
B) False

A

B) False

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3
Q

which of these tubes shows a positive result for urease?

A

C

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4
Q

Which enzymes cut the bonds at C?

A) phenylalanine deaminase and urease
B) urease and tryptophanase
C) cysteine desulfhydrase and thiosulfate reductase
D) lysine decarboxylase and phenylalanine deaminase

A

C) cysteine desulfhydrase and thiosulfate reductase

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5
Q

Which of the following has the correct order for the steps of the Gram stain?

A) Crystal Violet, Grams Iodine, Decolorizer, Safranin
B) Grams Iodine, Crystal Violet, Decolorizer, Safranin
C) Safranin, Grams Iodine, Decolorizer, Crystal Violet
D) Decolorizer, Safranin, Crystal Violet, Grams Iodine

A

A) Crystal Violet, Grams Iodine, Decolorizer, Safranin

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6
Q

A positive phenylalanine decarboxylase result indicates pathogenicity.

A) True
B) False

A

B) False

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7
Q

What is the differential ingredient in the phenylalanine deaminase test?

A) phenylalanine
B) lysine
C) deaminase
D) urea

A

A) phenylalanine

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8
Q

Which enzyme cuts the two bonds at D?

A) phenylalanine deaminase
B) tryptophanase
C) lysine decarboxylase
D) cysteine desulfhydrase

A

B) tryptophanase

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9
Q

What is the pH indicator in lysine decarboxylase media?

A) methyl red
B) phenol red
C) neutral red
D) bromcresol purple

A

D) bromcresol purple

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10
Q

Which tube is negative for phenylalanine deaminase?

A

C

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11
Q

Which tube(s) are positive for motility?

A) B only
B) D only
C) C and D
D) C only

A

C) C and D

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12
Q

Gram(-) cells stain purple and Gram(+) cells stain pink.

A) True
B) False

A

B) False

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13
Q

What must be added to the phenylalanine deaminase tubes after incubation?

A) 5 drops of mineral oil
B) Kovac’s reagent
C) phenol red
D) 4 drops of ferric chloride

A

D) 4 drops of ferric chloride

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14
Q

Which tube(s) are negative for urease?

A) a and d
B) b and c
C) c and d
D) a, c, and d

A

A) a and d

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15
Q

Protein degradation will usually _____ the pH of the media, while carbohydrate catabolism will lower the pH.

A

Increase

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16
Q

Which of the following are signs of bacterial motility in a SIM tube? (check all that apply)

A) black media
B) cloudy media
C) clear media

A

A) black media
B) cloudy media

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17
Q

The lysine decarboxylase media is a

A) plate
B) broth
C) deep
D) slant

A

B) broth

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18
Q

Is the urease media selective, differential or both?

A) differential
B) selective
C) both selective and differential

A

A) differential

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19
Q

What is the differential ingredient in urease media?

A) Lysine
B) Urea
C) Tryptophan
D) Phenylalanine

20
Q

Which tube below is positive for cysteine desulfhydrase and/or thiosulfate reductase?

21
Q

Proteins are broken down into ______ _______.

A

amino acids

22
Q

What is added to the SIM tube after incubation?

A) 5 drops of mineral oil
B) 4 drops of ferric chloride reagent
C) 4 drops of Methyl Red
D) 4 drops of Kovac’s reagent

A

D) 4 drops of Kovac’s reagent

23
Q

You are looking at bacterial cells at 1000X TM, where the field diameter is 0.2 mm. You estimate that 25 bacterial cells can fit across the diameter. What is the estimated length of one cell in mm? (Ok to use a calculator for this problem).

A

(0.008 mm, 0.008, .008)

24
Q

Which tube(s) are positive for urease?

A) b and c
B) b only
C) b, c and a
D) a and d

A

A) b and c

25
Q

Is phenylalanine media selective, differential or both?

A) differential
B) both
C) selective

A

A) differential

26
Q

Is the Lysine decarboxylase media selective or differential or both?

A) both selective and differential
B) differential
C) selective

A

B) differential

27
Q

Which of these tube(s) is positive for lysine decarboxylase?

28
Q

Which enzyme breaks the amino acid at A?

A) lysine decarboxylase
B) phenylalanine deaminase
C) Urease
D) tryptophanase

A

B) phenylalanine deaminase

29
Q

Which are the three differential materials in SIM? (click all that apply)

A) lysine
B) tryptophan
C) cysteine
D) thiosulfate

A

B) tryptophan
C) cysteine
D) thiosulfate

30
Q

Which tube(s) show really obvious indications of pathogenicity?

A) A and C
B) B and D
C) A only
D) C only

A

A) A and C

31
Q

A positive lysine decarboxylase result indicates pathogenicity.

A) True
B) False

32
Q

Which tube indicates a light positive for urease?

33
Q

The urease media is a

A) deep
B) broth
C) plate
D) slant

34
Q

What must be added to the lysine decarboxylase tube before incubation?

A) 3 drops of phenol red
B) 4 drops of Kovac’s reagent
C) 5 drops of mineral oil
D) 4 drops of ferric chloride

A

C) 5 drops of mineral oil

35
Q

Gram(+) cells stain purple and Gram(-) cells stain pink.

A) True
B) False

36
Q

Which inoculating tool is used to inoculate the phenylalanine deaminase media?

A) loop
B) sneeze
C) needle
D) swab

37
Q

You are looking at bacterial cells at 1000X TM, where the field diameter is 0.2 mm. You estimate that 50 bacterial cells can fit across the diameter. What is the estimated length of one cell in mm? (Ok to use a calculator for this problem)

38
Q

Which of these tube(s) contain a possible pathogen?

A) A and D
B) B, C, and D
C) B and C
D) B

A

C) B and C

39
Q

What enzyme cleaves the amino acid at B?

A) tryptophanase
B) phenylalanine deaminase
C) cysteine desulfhydrase
D) lysine decarboxylase

A

D) lysine decarboxylase

40
Q

which of these tubes shows a negative result for lysine decarboxylase?

A) B and C
B) A
C) B
D) C

A

A) B and C

41
Q

The SIM media is a

A) broth
B) slant
C) deep
D) plate

42
Q

What is the differential ingredient in the lysine decarboxylase tube?

A) cysteine
B) tryptophan
C) lysine
D) decarboxylase

43
Q

The phenylalanine deaminase media is a

A) broth
B) plate
C) deep
D) slant

44
Q

What is the pH indicator in the urease media?

A) phenol red
B) neutral red
C) Kovac’s reagent
D) bormcresol purple

A

A) phenol red

45
Q

Which SIM tube is positive for tryptophanase?

Microbiology Lab (9 decks)

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