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MT6320 BACTERIOLOGY LECTURE > UNIT 10: NON-ENTERICS - VIBRIO AND GR (-) BACILLI > Flashcards

UNIT 10: NON-ENTERICS - VIBRIO AND GR (-) BACILLI Flashcards

1
Q

All Vibrio spp. are halophilic except (2)

A

V. cholerae
V. mimicus

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2
Q

T/F

All Vibrio spp. can grow in 1% NaCl

A

True

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3
Q

T/F

All Vibrio spp. can grow in up to 7% NaCl

A

False

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4
Q

Gelatinase (+)

a. Vibrio
b. Aeromonas
c. Campylobacter
d. Helicobacter

A

a. Vibrio

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5
Q

All Vibrio spp. are nitrate reducers except (1)

A

V. metschnikovii

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6
Q

Indole (+)

a. Vibrio
b. Aeromonas
c. Campylobacter
d. Helicobacter

A

b. Aeromonas

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7
Q

Aeromonas

Lactose fermenter

A

A. caviae

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8
Q

Aeromonas

All have polar flagella except

A

A. salmonicida

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9
Q

Aeromonas

The only psychrophilic Aeromonas sp.

A

A. salmonicida

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10
Q

Aeromonas shows what type of hemolysis?

A

Beta-hemolytic

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11
Q

Common medical isolates of Vibrio spp. (2)

A

V. alginolyticus
V. vulnificus

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12
Q

Used in observation of Kanagawa Phenomenon

A

V. parahaemolyticus

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13
Q

T/F

All Vibrio spp. are glucose fermenters

A

True

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14
Q

Classic biotype vs El Tor biotype

Polymyxin B resistant

A

El Tor

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15
Q

Seventh pandemic of V. cholerae

A

El Tor biotype

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16
Q

Vibrio: Sucrose fermenter (3)

A

V. cholerae
V. alginolyticus
V. fluvialis

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17
Q

Vibrio: Mannitol fermenter

A

V. parahaemolyticus

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18
Q

Vibrio

All Vibrio spp. are positive for string test except (1)

A

V. parahaemolyticus

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19
Q

Vibrio: Lactose fermenter

A

V. vulnificus

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20
Q

Vibrio: ONPG (+)

A

V. vulnificus

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21
Q

Vibrio: LDC (+)

A

All except V. fluvialis

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22
Q

Vibrio: ODC (+)

A

All except V. fluvialis

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23
Q

Vibrio: ADH (+)

A

V. fluvialis

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24
Q

Vibrio: Voges-Proskauer (+)

A

V. alginolyticus

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25
Q

Vibrio: Voges-Proskauer (-)

A

V. fluvialis

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26
Q

Vibrio: Citrate (+)

A

V. fluvialis

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27
Q

Specimen for Vibrio spp. (2)

A

Stool and rectal swab
Strips of blotting paper soaked in liquid stool

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28
Q

Transport medium for Vibrio spp.

A

Cary-Blair

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29
Q

Culture media for Vibrio spp. (5)

A

Alkaline peptone water
BAP
Nutrient Agar
TCBS
MacConkey

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30
Q

Culture media for Vibrio spp.

Contains:
1% NaCl (pH 8.5)

A

Alkaline peptone water

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31
Q

Culture media for Vibrio spp.

Alkaline peptone water has 1% NaCl with a pH of

A

8.5

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32
Q

Culture media for Vibrio spp.

BAP and Nutrient Agar have what % of NaCl?

A

0.5% NaCl

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33
Q

Culture media for Vibrio spp

Appearance of Vibrio in BAP and CAP

A

Medium to large colonies; smooth, opaque with greenish blue hue

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34
Q

Culture media for Vibrio spp.

Appearance of Virbio in MacConkey Agar

A

Colorless colonies

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35
Q

Non-halophilic Vibrio spp. (2)

A

V. cholerae
V. mimicus

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36
Q

Pandemic strain of V. cholerae

Ogawa, Inaba, Hikojima

A

O1

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37
Q

Endemic strain of V. cholerae

India, Bangladesh

A

O139

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38
Q

Toxin virulence factor of V. cholerae (2)

A

Choleragen
Zona occludens toxin

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39
Q

Subunits of choleragen (2)

A

A subunits
B subunits

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40
Q

V. cholerae motility - produces what?

A

Mucinase

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41
Q

T/F

V. cholerae is an agent of bioterrorism

A

True

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42
Q

Mainstay treatment of V. cholerae

A

Oral and IV hydration

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43
Q

Motility of V. cholerae

A

Darting or Shooting star motility

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44
Q

T/F

V. cholerae can grow in the absence of NaCl

45
Q

Vibrio

Reagent used in string test

A

0.5% Sodium Deoxycholate

46
Q

(+) result of string test

A

Loss of turbidity and viscous, string-like appearance

Due to bacterial cell lysis

47
Q

Kanagawa phenomenon (+) result

A

Double zone hemolysis
“Patched Beta Hemolysis”

Resembles a cow’s pattern

48
Q

T/F

V. parahaemolyticus is medically relevant

49
Q

T/F

All Vibrio spp. grow in TCBS

50
Q

Purpose of bile in TCBS

A

Inhibits growth of Gr (+) organisms

51
Q

Unable to ferment inositol

52
Q

Hallmark difference of Vibrio and Aeromonas

A

Aeromonas cannot grow in 6% NaCl

53
Q

Frequently isolated Aeromonas spp. (2)

A

A. caviae
A. hydrophila

54
Q

Causes red leg disease among frogs

55
Q

Virulence factors of A. hydrophila

A

Heat-labile enterotoxin
Heat-stable cytotoxic enterotoxin

56
Q

Used to differentiate Aeromonas and Yersinia

A

Cefsulodin-Irgasan-Novobiocin (CIN)

57
Q

Can grow in CIN

58
Q

Characteristic microscopic appearance of Campylobacter

A

Seagull wing-shaped

59
Q

T/F

Campylobacter can utilize carbohydrates

A

False

Asaccharolytic

60
Q

Microaerophilic and Capnophilic (3)

A

Campylobacter
Helicobacter
Arcobacter butzleri

61
Q

Facultative Anaerobes

a. Vibrio
b. Aeromonas
c. Campylobacter
d. Helicobacter

A

b. Aeromonas

62
Q

Facultative Anaerobes or Aerobes

a. Vibrio
b. Aeromonas
c. Campylobacter
d. Helicobacter

63
Q

Majority of Aeromonas spp. are?

A

Mesophilic

64
Q

Causes Guillain Barre Syndrome

65
Q

Most common cause of gastroenteritis worldwide

66
Q

Has a MoT of ingestion of contaminated poultry products, drinking water, unpasteurized milk

67
Q

Campylobacter: Common cause of diarrhea among children and adults

A

C. jejuni subsp doylei

68
Q

Campylobacter: Isolated in blood cultures

A

C. fetus subsp fetus

69
Q

In Gram stain of Campylobacter, Safranin O is replaced by?

A

Carbol fuchsin

70
Q

Culture media of Campylobacter (4)

A

Campy-BAP
Butzler medium
Skirrow’s medium
Charcoal cefoperazone desoxycholate agar (CCDA)

71
Q

Culture media of Campylobacter

Campy-BAP contains (3)

A

Brucella agar
10% sheep RBC
Antibiotics

72
Q

Culture media of Campylobacter

Butzler medium contains

A

Thioglycollate fluid
10% RBC
Antibiotics

73
Q

Culture media of Campylobacter

Skirrow’s medium contains (3)

A

Oxoid Bld agar base
Lysed defibrinated horse RBC
Antibiotics

74
Q

Bow-shaped Gr (-) bacilli

A

Arcobacter butzleri

75
Q

Oxidase (+) only

A

Arcobacter butzleri

76
Q

Natural habitat of H. pylori

A

Human stomach

77
Q

Possess multiple polar flagella (4-6)

A

Helicobacter pylori

78
Q

Strong urease activity (+)

A

Helicobacter pylori

79
Q

Major cause of type B gastritis

80
Q

Important risk factor for gastric carcinoma

81
Q

Specimen for Helicobacter spp. (2)

A

Gastric biopsy materials
Urine

82
Q

Direct examination of Helicobacter is done in what specimen?

A

Gastric biopsy materials

83
Q

Transport medium of biopsy materials for Helicobacter

A

Stuart’s Medium

84
Q

Culture media for Helicobacter (4)

A

CAP
Skirrow’s medium
Cysteine brucella broth with 20% glycerol
5% sheep’s blood brucella agar

85
Q

Helicobacter

20% glycerol in Cysteine brucella broth is frozen at what temp?

A

-70 degrees C

86
Q

Stains to visualize Helicobacter (3)

A

Gram’s
Warthin Starry
Silver stains

87
Q

Uses urea breath test

88
Q

Gold standard for Helicobacter

A

Histologic Staining and Culture

89
Q

Hippurate hydrolysis (+)

90
Q

Nitrate reducers (5)

A

C. jejuni
C. coli
C. fetus
Arcobacter butzleri
Vibrio

91
Q

Growth at 15C

A

Arcobacter butzleri

92
Q

Growth at 25C (2)

A

Arcobacter butzleri
Campylobacter fetus

93
Q

Growth at 42C

94
Q

Nalidixic acid susceptible

95
Q

Nalidixic acid resistant

96
Q

Cephalothin susceptible (2)

A

H. pylori
C. fetus

97
Q

Cephalothin resistant

98
Q

Growth at 37C

A

C. jejuni
C. coli
C. fetus

99
Q

T/F

C. fetus can grow at 42C

100
Q

Family Enterobacteriaceae are oxidase (-) except

A

Plesiomonas

101
Q

Inositol (+)

A

Plesiomonas

102
Q

Bile solubility (+)

A

S. pneumoniae

103
Q

Colony with black centers in CIN agar

104
Q

Bacterial clumping with formation of a black center due to H2S production and brown diffusible pigment

A

Enterobacter cloacae

105
Q

Brown diffusible pigment of E. cloacae is produced by

A

Phenylalanine deaminase

106
Q

Next most common nosocomial infection to Pseudomonas

A

Acinetobacter

107
Q

Notorious for nosocomial infections, drug resistance, and biofilm formation

A

Pseudomonas

108
Q

Causes glanders and meliodosis

A

Burkholderia

MT6320 BACTERIOLOGY LECTURE (1 decks)

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